WU Hong,LU Hui,GAO Keli,et al.Effects of Growth Regulators on Rooting Characteristics and Protective Enzyme System of Cyclocarya paliurus[J].Northern Horticulture,2019,43(20):106-111.[doi:10.11937/bfyy.20191100]
生长调节剂对青钱柳扦插生根及生根过程中保护酶系统的影响
- Title:
- Effects of Growth Regulators on Rooting Characteristics and Protective Enzyme System of Cyclocarya paliurus
- 文献标志码:
- A
- 摘要:
- 以3年生青钱柳(Cyclocarya paliurus)种子繁殖母株上的一年生木质化枝条为扦插材料,以1/3珍珠岩+1/3蛭石+1/3河沙为扦插基质,以不经任何处理的插穗为对照,研究ABT1、ABT2和特效生根粉不同浓度(100、200、300、500 mg?L-1)对青钱柳扦插的影响,并分析扦插生根过程中氧化酶活性的变化,以期筛选出青钱柳硬枝扦插的适宜条件和方法。结果表明:生长调节剂处理可不同程度提高青钱柳扦插的生根率,其中ABT2效果较好,200 mg?L-1时生根率可达78%;青钱柳扦插整个生根过程中ABT2处理组多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)活性呈现“上升-下降-上升-下降”的趋势;过氧化物酶(POD)活性呈“上升-下降-上升”的变化趋势。但与对照相比变化趋势明显,平均周期提前5 d左右。
- Abstract:
- In order to screen out the suitable conditions and methods for hard cutting of Cyclocarya paliurus.Hardwood shoots from 3-year-old Cyclocarya paliurus tree were taken as cutting materials,1/3 perlite+1/3 vermiculite+1/3 sand were taken as cutting matrix and the untreated cuttings as control.The effects of different concentrations of ABT1,ABT2 and specific rooting powder were studied,and the changes of IAAO,PPO and POD activities were analyzed.The results showed that the growth regulator treatment could increase the rate of rooting of Cyclocarya paliurus cuttings,in which,the survival rate treated with 200 mg?L-1 ABT2 was the highest,which reached to 78%;treated with 200 mg?L-1 ABT2,the activity of IAAO and PPO showed a trend of ‘rise-descend-rise-descend’,and the activity of POD showed a trend of ‘rise-descend-rise’.And the change trend was obvious compared with the control.The average rooting cycle was about 5 days ahead of time.
参考文献/References:
[1]方升佐,洑香香.青钱柳资源培育与开发利用的研究进展[J].南京林业大学学报(自然科学版),2007(1):95-100.[2]温晓梨,蔡芳燕.青钱柳药理活性研究进展[J].亚太传统医药,2017,13(20):88-90.[3]刘盈盈,张玉武,张珍明,等.青钱柳资源培育及其开发利用研究进展[J].北方园艺,2015(20):193-196.[4]尚旭岚,徐锡增,方升佐.青钱柳种子次生休眠的发生及贮藏物质的变化[J].南京林业大学学报(自然科学版),2006(2):99-102.[5]杨万霞,洑香香,方升佐.青钱柳种子的种皮构造及其对透水性的影响[J].南京林业大学学报(自然科学版),2005(5):25-28.[6]尚旭岚,方升佐,徐锡增.综合处理措施对解除青钱柳种子休眠的影响[J].中南林业科技大学学报,2014,34(1):42-48.[7]李先民,方升佐,刘晶晶.青钱柳扦插育苗试验[J].林业科技开发,2014,28(5):124-127.[8]姚锐,王义强,谭益民,等.激素处理对北美香柏扦插繁殖及相关氧化酶活性的影响[J].江苏农业科学,2017,45(11):97-102.[9]李先民.青钱柳扦插繁殖技术及其生根机理的研究[D].南京:南京林业大学,2014.[10]朱永超,李彬,廖伟彪.3种生长素对蓝叶忍冬枝条扦插生根的影响[J].草业科学,2016,33(1):61-66.[11]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,1999.[12]郝建军,康宗利,于洋.植物生理学实验技术[M].北京:化学工业出版社,2007.[13]张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2003.[14]王文英,翁和录,刑世岩.植物生长调节剂在扦插繁殖中的应用[J].林业实用技术,2002(7):9-10.[15]张丹丹,范俊俊,赵明明,等.外施植物生长调节剂对细柄阿丁枫扦插生根的影响[J].南京林业大学学报(自然科学版),2018,42(1):41-47.[16]张学顺,张英姿,周宁宁,等.不同浓度ABT生根粉对连翘扦插育苗的影响研究[J].信阳农业高等专科学校学报,2011,21(3):103-104,108.[17]余明光.不同浓度ABT生根粉对红花檵木嫩枝扦插生根影响[J].现代园艺,2012(3):3-4,14.[18]刘文盈.不同质量浓度生根粉对花蓼扦插生根的影响[J].河南农业科学,2013,42(12):112-114.[19]张静兰,徐桂芳,唐定台,等.吲哚乙酸、吲哚乙酸氧化酶和几种愈伤组织生长的关系[J].植物生理学报,1979(3):193-198.[20]姚瑞玲,项东云,陈健波,等.2种桉树插穗内酚酸类物及相关酶活性比较[J].桉树科技,2010,27(2):29-34.[21]王小玲,赵忠,权金娥,等.外源激素对四倍体刺槐硬枝扦插生根及其关联酶活性的影响[J].西北植物学报,2011,31(1):116-122.[22]王政,王照路,申萍,等.牡丹试管苗与扦插苗生根过程中相关酶活性的变化[J].西北农林科技大学学报(自然科学版),2014,42(10):193-198.[23]颜婷美,张安琪,王峰,等.绣球丁香扦插生根过程中内源激素变化[J].中国农学通报,2014,30(13):186-189.[24]宋金耀,宋刚,李辉,等.几种园艺植物扦插生根过程中生化指标的变化[J].江苏农业科学,2010(3):211-214.[25]王宏信,李向林,王楠,等.ABT1处理对降香黄檀硬枝扦插生根及其关联酶活性的影响[J].江苏农业科学,2017,45(8):138-141.[26]付喜玲,郭先锋,康晓飞,等.IBA对芍药扦插生根的影响及生根过程中相关酶活性的变化[J].园艺学报,2009,36(6):849-854.[27]徐丽萍,上官新晨,喻方圆.秤锤树嫩枝扦插过程中几种酶活性变化研究[J].江西农业大学学报,2009,31(2):274-277.
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备注/Memo
第一作者简介:吴红(1979-),女,甘肃张掖人,硕士,讲师,研究方向为林木遗传育种与繁育。E-mail:wh02gs@126.com.责任作者:李勇军(1975-),男,江苏宜兴人,博士,教授,研究方向为中药学。E-mail:yf6011@hotmail.com.基金项目:中央财政林业科技推广示范资助项目(苏[2017]TG 05号);江苏省林业科技创新与推广资助项目(LYKJ[2018]33);江苏省大学生创新创业训练计划资助项目(201812806028H);泰州市科技支撑计划(农业)资助项目TN201810(201812806028H)。收稿日期:2019-04-30