ZHANG Wenxiang,CHEN Tianlan,ZHANG Rongxiang,et al.Genome-wide Identity and Bioinformatic Analysis of ARF Transcription Factors in Rhododendron delavayi[J].Northern Horticulture,2019,43(24):74-80.[doi:10.11937/bfyy.20191097]
马缨杜鹃ARF转录因子家族的全基因组鉴定与生物信息学分析
- Title:
- Genome-wide Identity and Bioinformatic Analysis of ARF Transcription Factors in Rhododendron delavayi
- Keywords:
- Rhododendron delavayi; ARF; bioinformatics analysis
- 文献标志码:
- A
- 摘要:
- 以马缨杜鹃（Rhododendron delavayi）基因组数据为基础，以拟南芥ARF（AtARF）序列为参考，利用Blast比对程序，在马缨杜鹃基因组数据库中鉴定了15个ARF基因，命名为RdARF。使用生物信息学的方法对基因的结构、理化性质、系统进化关系以及表达模式进行了分析，以期为马缨杜鹃ARF基因的克隆及深入研究提供参考。结果表明:15个RdARF蛋白均含有保守的B3结构域和Auxin-resp结构域，部分蛋白含有Aux/IAA结构域，共有10个保守基序，均含有丝氨酸(Ser)、苏氨酸(Thr)和酪氨酸(Tyr)磷酸化位点，均含有内含子和外显子结构，与23个AtARF蛋白一起可以分为Ia、Ib、IIa、IIb 和III 5个亚家族，15个RdARF基因在干旱胁迫前后具有4种不同的表达模式。
- Abstract:
- Based on the genomic data of Rhododendron delavayi，15 ARF genes were identified by blast comparison program using Arabidopsis ARFs (AtARF) as references，named RdARF.In order to in-depth study or further clone these genes，bio-informatics analysis was carried out to analyze their structures，physio-chemical properties and phylogenetic relationships.The results showed that all the 15 ARF genes of Rhododendron delavayi contained introns and exons，conservative B3 and Auxin-resp domains，serine (Ser.)，threonine (Thr.) and tyrosine (Tyr.) phosphorylation sites，and there were 10 kinds of conserved motifs totally in 15 RdARF proteins，five subgroups (Ia，Ib，IIa，IIb and III) between 15 RdARF and 23 AtARF and 4 different expression patterns response to drought stress.
参考文献/References:
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备注/Memo
第一作者简介：张文香（1984-），女，河北衡水人，博士，副教授，现主要从事植物生理与分子生物学等研究工作。E-mail：zwx26_2006@126.com.责任作者：王自布（1984-），男，河南驻马店人，博士，副教授，现主要从事花卉育种等研究工作。E-mail:2609151292@qq.com.基金项目：贵州省自然科学技术基金资助项目(黔科合J字[2014]2144号)；贵州师范学院博士科研启动基金资助项目(13BS020)。收稿日期：2019-05-06