[1]袁云香.H2O2对孔雀草悬浮细胞中黄酮含量及抗氧化酶活性的影响[J].北方园艺,2019,43(19):55-59.[doi:10.11937/bfyy.20190840]
　YUAN Yunxiang.Effects of Hydrogen Peroxide on Flavonoid Content and Antioxidant Enzymes Actitvty of Suspension Cells of Tagetes patula L.[J].Northern Horticulture,2019,43(19):55-59.[doi:10.11937/bfyy.20190840]
点击复制

H2O2对孔雀草悬浮细胞中黄酮含量及抗氧化酶活性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年19 页码: 55-59 栏目: 出版日期: 2019-10-15
Title:
Effects of Hydrogen Peroxide on Flavonoid Content and Antioxidant Enzymes Actitvty of Suspension Cells of Tagetes patula L.
作者:
袁云香1; 2
（1.渭南师范学院 农商学院，陕西 渭南 714099；2.陕西省煤基低碳醇转化工程研究中心，陕西 渭南 714099）
Author(s):
YUAN Yunxiang1; 2
(1.College of Agriculture and Commerce,Weinan Normal University，Weinan，Shaanxi 714099；2.Shaanxi Province Engineering Research Center of Coal Conversion Alcohol，Weinan，Shaanxi 714099)
关键词:
孔雀草; H2O2; 黄酮; 悬浮细胞; 抗氧化酶
Keywords:
Tagetes patula L.; hydrogen peroxide; flavonoid; suspension cell; antioxidant enzymes
DOI:
10.11937/bfyy.20190840
文献标志码:
A
摘要:
以孔雀草悬浮细胞为试材，在液体培养基中附加不同浓度的H2O2进行培养，研究H2O2对孔雀草悬浮细胞黄酮代谢、抗氧化酶活性的影响。结果表明：在H2O2浓度为0~10 mmol?L-1时，孔雀草悬浮细胞总黄酮含量均呈现上升趋势，其中10 mmol?L-1的 H2O2处理细胞总黄酮含量最高，而高浓度H2O2处理时总黄酮含量均呈下降趋势。孔雀草悬浮细胞抗氧化酶活性变化也是随着H2O2浓度呈现先上升后下降的趋势，0~10 mmol?L-1范围内丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性、过氧化氢酶（CAT）活性、过氧化物酶（POD）活性和脯氨酸含量显著增高，15~25 mmol?L-1范围内的MDA含量、SOD活性、CAT活性、POD活性和脯氨酸含量呈下降趋势。
Abstract:
The suspension cell of Tagetes patula L.were taken as test materials and cultured in liquid medium which was added with different concentration H2O2 to study the effects of exogenous hydrogen peroxide on flavonoid content and antioxidant enzymes actitvty of suspension cells of of Tagetes patula L..The results showed that the content of total flavonoids in suspension cells of Tagetes patula L.showed an upward trend within the range of 0-10 mmol?L-1 H2O2，it had the highest with 10 mmol?L-1 H2O2，and with high concentration of H2O2 showed a declining trend.The change of antioxidant enzyme activity also showed a trend of increasing and then decreasing with the concentration of H2O2.The MDA content，SOD activity，CAT activity，POD activity and proline content were significantly increased in the range of 0-10 mmol?L-1 H2O2 and decreased in the range of 15-25 mmol?L-1 H2O2.

参考文献/References:

[1]POLITI A S，FIGUEIRA M，ARAUJO A M，et al.Acaricidal activity of ethanolic extract from aerial parts of Tagetes patula L.(Asteraceae) against larvae and engorged adult females of Rhipicephalus sanguineus (Latreille，1806)[J].Parasites and Vectors，2012(5):295.[2]袁云香.响应面优化孔雀草悬浮细胞总黄酮的提取工艺[J].食品工业科技，2015，36(10)：220-224，228.[3]张志良.植物生理学实验指导[M].4版.北京:高等教育出版社,1990.[4]高俊凤.植物生理学实验技术[M].西安:世界图书出版公司，2000.[5]杨芳，张宗申，杨斌.过氧化氢对丹参悬浮细胞生长及次生代谢物积累的影响[J].西南农业学报，2011，24(3)：901-904.[6]陈颖，杨华，乐利，等.过氧化氢对银杏悬浮细胞黄酮含量及抗氧化酶活性的影响[J].中南林业科技大学学报，2014，34(12)：40-45，55.[7]邓云云，张俊娥.H2O2对杜仲愈伤组织中黄酮含量及过氧化物酶活性的影响[J].分子植物育种，2018，16(3)：1007-1010.[8]李汝佳，李雪梅.水杨酸、脱落酸和过氧化氢对镉胁迫小麦幼苗光合及抗氧化酶活性的影响[J].生态学杂志，2007，26(12)：2096-2099.

相似文献/References:

[1]谢修鸿,王晓红,付泓,等.孔雀草水提液对多年生黑麦草种子萌发的影响[J].北方园艺,2013,37(13):81.
　XIE Xiu-hong,WANG Xiao-hong,FU Hong,et al.Effect of Tagetes patula L.Aqueous Extract Solution on Seed Germination of Perennial Ryegrass[J].Northern Horticulture,2013,37(19):81.
[2]张立磊,王少平.盐胁迫对孔雀草种子发芽的影响[J].北方园艺,2012,36(23):86.
　ZHANG Li-lei,WANG Shao-ping.Effect of Salt Stress on the Seed Germination of Tagetes patula[J].Northern Horticulture,2012,36(19):86.
[3]谢修鸿,刘玉伟,王晓红,等.孔雀草水提液对自身生长及土壤微生物数量和酶活性的影响[J].北方园艺,2015,39(06):157.[doi:10.11937/bfyy.201506044]
　XIE Xiu-hong,LIU Yu-wei,WANG Xiao-hong,et al.Effect of Water Extraction Liquid of Tagetes patula L.on Itself Growth and Microbial Quantity and Enzymatic Activity[J].Northern Horticulture,2015,39(19):157.[doi:10.11937/bfyy.201506044]
[4]高 荣 侠.人工授粉对不同品种孔雀草结实率的影响[J].北方园艺,2011,35(05):0.[doi:10.11937/bfyy.201105040]
　GAO Rong -xia.Influence of Man-pollinating on Setting Percentage of Different Varieties Tagetes patula[J].Northern Horticulture,2011,35(19):0.[doi:10.11937/bfyy.201105040]
[5]梁 顺 祥,郭 洋 楠,唐 道 城,等.孔雀草 POD 同工酶及主要观赏性状研究[J].北方园艺,2009,33(09):0.[doi:10.11937/bfyy.200909025]
　LIANG Shun-xiang,GUO Yang -nan,TANG Dao -cheng,et al.Study on Peroxidase Isozyme and Main Ornamental Characteristics of Tagetes patula Breeds[J].Northern Horticulture,2009,33(19):0.[doi:10.11937/bfyy.200909025]
[6]高群英,于占晶,孙文清,等.干旱胁迫对三种菊科植物种子萌发的影响[J].北方园艺,2020,44(16):56.[doi:10.11937/bfyy.20193460]
　GAO Qunying,YU Zhanjing,SUN Wenqing,et al.Effects of Drought Stress on Seed Germination of Three Compositae Species[J].Northern Horticulture,2020,44(19):56.[doi:10.11937/bfyy.20193460]
[7]刘瑞霞,张先平,佘波,等.外源H2O2对复盐胁迫下木槿的生理响应机制[J].北方园艺,2021,(04):72.[doi:10.11937/bfyy.20200525]
　LIU Ruixia,ZHANG Xianping,SHE Bo,et al.Physiological Response Mechanism of Exogenous H2O2 to Hibiscus syriacus Linn.Under Double Salt Stress[J].Northern Horticulture,2021,(19):72.[doi:10.11937/bfyy.20200525]
[8]韩淑梅,陈琳,刘玉婷,等.外源水杨酸对镉胁迫下孔雀草生理特性的影响[J].北方园艺,2021,(09):68.[doi:10.11937/bfyy.20203196]
　HAN Shumei,CHEN Lin,LIU Yuting,et al.Effects of Exogenous Salicylic Acid on the Physiological Characteristics of Tagetes patula L.Under Cadmium Stress[J].Northern Horticulture,2021,(19):68.[doi:10.11937/bfyy.20203196]
[9]王玲丽,石永亮,李小珊,等.混合盐碱胁迫对孔雀草生长的影响[J].北方园艺,2021,(12):63.[doi:10.11937/bfyy.20203908]
　WANG Lingli,SHI Yongliang,LI Xiaoshan,et al.Effects of Mixed Saline-alkali Stress on Growth of Tagetes patula[J].Northern Horticulture,2021,(19):63.[doi:10.11937/bfyy.20203908]

备注/Memo

作者简介：袁云香（1980-），女，硕士，副教授，研究方向为植物逆境生理。E-mail:yuanyunxiang2006@126.com.基金项目：国家自然科学基金资助项目（31000410）；渭南师范学院特色学科建设资助项目（14TSXK04）；渭南师范学院教改资助项目（JG201930）；陕西省教育厅科学研究计划资助项目（18JS036)。收稿日期：2019-04-29

更新日期/Last Update: 2019-10-09