MENG Lulu,YAN Wenhan,SUN Cuicui,et al.Influences of Temperature and pH on Growth and Toxins Production of Valsa mali[J].Northern Horticulture,2019,43(19):6-12.[doi:10.11937/bfyy.20190385]
温度和pH对苹果树腐烂病菌生长及产毒素水平的影响
- Title:
- Influences of Temperature and pH on Growth and Toxins Production of Valsa mali
- Keywords:
- Valsa mali; temperature; pH; mycelial growth; toxin levels
- 文献标志码:
- A
- 摘要:
- 以强致病力菌株LXS080601为试材，采用生长速率法和高效液相色谱法，对腐烂病菌LXS080601在不同温度（5~35 ℃）及pH（pH 3~9）条件下菌丝生长、产孢能力及产毒素水平进行了测定，以期探明温度和pH对苹果树腐烂病菌生长及产毒素水平的影响。结果表明：苹果树腐烂病菌LXS080601在5~35 ℃和pH 3~9范围内均可生长，最适生长温度为25~30 ℃，最适pH为4~6；pH对苹果树腐烂病菌产孢也有明显的影响，最适pH为5~6，分生孢子器数量达每皿315个。该菌株在苹果树皮培养基中最适宜产毒素温度为20~25 ℃，毒素总量最高可达128.06 μg?mL-1，10~35 ℃条件下均可产生5种毒素，而5 ℃时仅产生3种毒素，毒素总量仅为8.46 μg?mL-1；最适产毒素pH为3~6，pH为9时仅检测到4种毒素，且毒素总量最低。
- Abstract:
- To explore the influences of temperature (5-35 ℃) and pH (3-9) on mycelial growth，sporulation ability and toxins production of Valsa mali，the strain LXS080601 with high virulence was studied by using growth rate method and high performance liquid chromatography (HPLC).The results showed that the strain LXS080601 was able to grow at a temperature range from 5 ℃ to 35 ℃ and at a pH range from 3 to 9.The optimal temperature and pH for LXS080601 ranged from 25 ℃ to 30 ℃ and from 4 to 6,optimal temperature and pH for LXS080601 ranged from 25 ℃ to 30 ℃ and from 4 to 6，respectively.pH also had significant effects on sporulation of V.mali.The optimal pH for LXS080601 sporulation ranged from 5 to 6，and the number of conidia per plate reached to 315.In apple bark extract medium，the optimal temperature for toxins production was from 20 ℃ to 25 ℃，and the total toxins amount was up to 128.06 μg?mL-1.Five kinds of toxins were produced by strain LXS080601 from 10 ℃ to 35 ℃，however，only three kinds of toxins were detected at 5 ℃ and the total toxins amount was only 8.46 μg?mL-1.In addition，the optimal pH for toxins production was from 3 to 6，while at pH 9 only four kinds of toxins were detected and the total toxins amount was the lowest.
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备注/Memo
第一作者简介：孟璐璐(1993-)，女，山东济宁人，硕士研究生，研究方向为果树病害。E-mail:menglulu19931128@163.com.责任作者：王彩霞(1977-)，女，山东德州人，博士，教授，硕士生导师，现主要从事果树病害等研究工作。E-mail:cxwang@qau.edu.cn.基金项目：山东省自然科学基金资助项目(ZR2018MC020);国家自然科学基金资助项目（31272001,31371883）；青岛农业大学研究生创新立项资助项目(QYC201810);青岛农业大学大学生创新训练资助项目。收稿日期：2019-04-30