[1]徐元江,刘江,苏艳杰,等.匙叶翼首草发根诱导与培养基筛选[J].北方园艺,2019,43(18):110-115.[doi:10.11937/bfyy.20183019]
　XU Yuanjiang,LIU Jiang,SU Yanjie,et al.Hairy Root Induction of Pterocephalus hookeri and Screening of Medium[J].Northern Horticulture,2019,43(18):110-115.[doi:10.11937/bfyy.20183019]

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匙叶翼首草发根诱导与培养基筛选

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷期数: 2019年18 页码: 110-115 栏目: 出版日期: 2019-09-30

Title:: Hairy Root Induction of Pterocephalus hookeri and Screening of Medium

作者:: 徐元江1; 2; 刘江1; 3; 苏艳杰1; 3; 权红2; 郑维列2; 兰小中1; 3; （1．西藏农牧学院西藏农牧学院-西南大学药用植物联合研发中心，西藏林芝 860000；2．西藏农牧学院高原生态研究所，西藏林芝 860000；3．西藏农牧学院食品科学学院，西藏林芝 860000）

Author(s):: XU Yuanjiang1; 2; LIU Jiang1; 3; SU Yanjie1; 3; QUAN Hong2; ZHENG Weilie2; LAN Xiaozhong1; 3; (1.TAAHC-SWU Medicinal Plants Joint R&D Centre,Tibet Agriculture and Animal Husbandry University，Nyingchi,Tibet 860000；2.Res,Institute of Tibet Plateau Ecology,Tibet Agriculture and Animal Husbandry University，Nyingchi,Tibet 860000；3.Food Science College,Tibet Agriculture and Animal Husbandry University，Nyingchi,Tibet 860000)

关键词:: 匙叶翼首草; 发根; 培养基; 齐墩果酸; 熊果酸; HPLC

Keywords:: Pterocephalus hookeri; hairy root; medium; oleanolic acid; ursolic acid; HPLC

DOI:: 10.11937/bfyy.20183019

文献标志码:: A

摘要:: 为筛选最佳匙叶翼首草（Pterocephalus hookeri (C.B.Clarke) Hock.）发根的生长培养基，以其无菌苗为试验材料，通过农杆菌C58C1(pRiA4)浸染叶片产生发根，研究不同侵染条件对发根数量的影响；采用液体培养的方式，通过测定鲜质量、干质量、齐墩果酸与熊果酸含量，验证5种培养基（分别为MS、1/4MS、B5、1/2B5、WPM）对翼首草发根生物量与含量的影响。结果表明：翼首草以浸染8 min，共培1 d的诱导率最高（12%）；发根在1/4MS培养基中生物量最大，鲜质量为9.210 g，干质量为0.529 g，其次为B5培养基鲜质量达5.270 g、干质量达0.385 g；翼首草发根的齐墩果酸与熊果酸含量在B5培养基最高，分别达2.314、3.213 mg?g-1。1/4MS最低，说明B5为翼首草发根生长与生产的最佳培养基。

Abstract:: This study was designed to screen for the optimal medium for root growth of (Pterocephalus hookeri (C.B.Clarke) Hock.).The sterile seedling of Pterocephalus hookeri was used as the research object.The roots were obtained by infecting the leaves with Agrobacterium tumefaciens C58C1 (pRiA4) to study the effects of different infestation conditions on the number of hairy roots.The effects of five mediums (MS,1/4MS,B5,1/2B5 and WPM) on the root biomass and content of oleanolic acid and vrsolic acid for Pterocphalus hookeri were veriflied by the determination of fresh weight、dry weight、oleanolic acid、ursolic acid content in liquid culture.The results showed that the induction rate of Pterocephalus hookeri was the highest (12%) for infect 8 minutes，co-culture 1 day,and the root biomass was the highest in 1/4MS liquid medium.The fresh weight was 9.210 g and the dry weight was 0.529 g.The fresh weight of B5 medium was 5.270 g and the dry weight was 0.385 g.The roots of oleanolic acid and ursolic acid content were the highest in B5 medium，which were 2.314 mg?g-1 and 3.213 mg?g-1，respectively.1/4MS was the lowest，indicating that B5 was the best medium for root growth and production of Pterocephalus hookeri.

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备注/Memo

第一作者简介：徐元江(1990-)，男，博士研究生，研究方向为藏药材分子生态学。E-mail：yuanjiangxu@126.com.责任作者：兰小中(1974-)，男，博士，教授，硕士生导师，现主要从事药用植物资源与开发利用等研究工作。E-mail：lanxiaozhong@163.com.基金项目：西藏特色农牧资源研发协同创新中心资助项目；第四次全国中药资源普查试点-西藏自治区中（藏）药资源普查资助项目（20180611-542226）。收稿日期：2019-02-13

更新日期/Last Update: 2019-10-08