ZHANG Lipeng,HAN Xiangyan,CHENG Lulu,et al.Isolation and Expression Analysis of Cyp450 Genes in Tibet Rhodiola renulata[J].Northern Horticulture,2019,43(18):116-127.[doi:10.11937/bfyy.20184635]
西藏大花红景天cyp450基因的分离与表达分析
- Title:
- Isolation and Expression Analysis of Cyp450 Genes in Tibet Rhodiola renulata
- Keywords:
- Rhodiola crenulata; cyp450; bioinformation; expression analysis
- 文献标志码:
- A
- 摘要:
- 以西藏大花红景天为研究材料，采用生物信息学和实时荧光定量PCR技术研究了细胞色素p450（cyp450）家族中16个基因成员的序列特征、蛋白质特性和表达模式，以期获得对Rccyp450基因家族的初步认识，填补该基因家族的研究空白。结果表明：16个Rccyp450基因开放阅读框大小均为1 374 bp以上，所编码的氨基酸序列长度为480~530个。序列比对结果显示Rccyp450共属于10个亚家族其中4个基因为A-type，12个基因为non-A type。蛋白激酶磷酸化位点预测结果显示Rccyp450基因均含有33个以上可磷酸化位点。实时定量PCR结果显示上述基因在根、茎、叶3个器官中的表达模式不同。且能够受到低温/紫外和多种植物激素（脱落酸、生长素、茉莉酸甲酯）的诱导表达。
- Abstract:
- Using Tibet Rhodiola crenulata as research materials,the cytochrome p450 (cyp450) gene family members were analyzed by bioinformatics and real-time PCR,which would be helpful to obtain preliminary understanding and fill in the research gap for this gene family.The results showed that the open reading frames of 16 Rccyp450 genes were all above 1 374 bp and the length of amino acid sequence were 480-530.Sequence alignment results showed that Rccyp450 genes belonged to 10 subfamilies,of which 4 genes were A type and 12 genes were non-A type.In addition,Rccyp450 genes contained more than 33 phosphate sites which indicated that they might be modified by phosphatases.The expression pattern for tissue and stresses presented that Rccyp450 genes expressed distinctly differently in three tissues and could be induced by low temperature/UA and three hormones included abscisic acid,auxin and jasmonic acid.
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备注/Memo
第一作者简介：张力鹏（1989-），男，河北沧州人，博士研究生，研究方向为分子细胞遗传学。E-mail:nknanhai@163.com.责任作者：宋文芹（1953-），女，天津人，博士，教授，现主要从事中国主要中药材种质资源与品种改良等研究工作。E-mail:songwq@nankai.edu.cn.收稿日期：2019-03-09