YUE Lei,SUN Yueli,YIN Baoying,et al.Effects of Exogenous Polyamines on Growth of Self-pollinated Pollen Tube and Self-crossing Fruit of Yali Pear[J].Northern Horticulture,2018,42(17):32-38.[doi:10.11937/bfyy.20180361]
外源多胺对鸭梨自花授粉花粉管生长及自交坐果的影响
- Title:
- Effects of Exogenous Polyamines on Growth of Self-pollinated Pollen Tube and Self-crossing Fruit of Yali Pear
- 文献标志码:
- A
- 摘要:
- 以鸭梨为试材，进行不同浓度外源多胺处理，通过离体花粉培养，荧光显微动态观察和花序、花朵坐果率调查，研究了外源多胺处理对鸭梨自花授粉花粉管生长及自交坐果的影响，以期为外源多胺调控鸭梨自交不亲和性提供重要依据。结果表明：适宜浓度的外源腐胺(Put)、亚精胺(Spd)、精胺(Spm)处理后的鸭梨花柱提取液，能够提高花粉萌发率和促进花粉管生长，最适宜的Put浓度和Spd浓度为0.250 mmol·L-1，Spm浓度为0.050 mmol·L-1。鸭梨自花授粉72 h后，花粉管先端膨大，在花柱中部停止生长。3种多胺处理均能使其穿过花柱中部到达基部，其中以0.250 mmol·L-1 Spd处理花粉管到达基部最多。不同浓度外源多胺均能提高鸭梨自花授粉花序坐果率和花朵坐果率，其中以0.250 mmol·L-1 Put表现最佳。
- Abstract:
- Yali pear was used as experimental material,and different concentrations of exogenous polyamines were treated.The effects of exogenous polyamines on growth of self-pollinated pollen tube and self-crossing fruit were studied by pollen culture in vitro,fluorescence microscopic observation and fruit setting investigation.The results showed that pollen germination rate in vitro and the pollen tube length were significantly increased after suitable concentration of polyamines treatment.The most suitable concentration of Put and Spd was 0.250 mmol·L-1,and the concentration of Spm was 0.050 mmol·L-1.Pollen tube stopped growth and enlarged at middle of the style after 72 hours of self-pollination.But it could go through the middle of the style to the bottom after three kinds of polyamines treatments.The Spd concentration with 0.250 mmol·L-1 was the best.The percentage of self-pollinated fruit set was significantly increased by spraying different kind of exogenous polyamines.The best effects of exogenous polyamine was 0.250 mmol·L-1 Put treatment.
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备注/Memo
第一作者简介：岳雷（1982-），男，河北沧州人，硕士，助理研究员，现主要从事果树结实生理与分子生物学等研究工作。E-mail:yuelei279@sohu.com.责任作者：徐继忠（1964-），男，河北唐山人，博士，教授，博士生导师，现主要从事果树生物技术及果树栽培生理与生态等研究工作。E-mail:xjzhxw@126.com.基金项目：河北省自然科学基金资助项目（C200600429）。收稿日期：2018-03-19