LIAN Huashan,SUN Guochao.Effects of Abscisic Acid Soaking Seeds on Growth and Physiological Characteristics of Radish Seedlings Under Aluminum Stress[J].Northern Horticulture,2018,42(05):14-19.[doi:10.11937/bfyy.20173310]
脱落酸浸种对铝胁迫下萝卜幼苗生长及生理特性的影响
- Title:
- Effects of Abscisic Acid Soaking Seeds on Growth and Physiological Characteristics of Radish Seedlings Under Aluminum Stress
- Keywords:
- abscisic acid; aluminum stress; growth; physiological characteristics
- 文献标志码:
- A
- 摘要:
- 以萝卜种子为试材,采用不同浓度脱落酸(ABA)溶液浸泡萝卜种子,研究了ABA浸种对铝胁迫下萝卜幼苗生长、光合作用及生理特性影响,以期为ABA缓解蔬菜铝胁迫机理研究提供参考依据。结果表明:低于20 μmol?L-1 ABA浸种处理能缓解铝胁迫对萝卜幼苗生长的抑制,提高铝胁迫下萝卜幼苗叶片光合色素含量,提高净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr),增强光合作用,同时提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性,降低丙二醛(MDA)的积累,保持细胞活性氧代谢平衡,增加可溶性蛋白质含量,缓解铝离子对植物细胞膜的伤害。因此,ABA浸种能提高萝卜幼苗的耐铝能力,且以5 μmol?L-1的ABA浸种时效果最佳。
- Abstract:
- Radish seeds were used as materials,the effects of abscisic acid (ABA) soaking seeds on growth,photosynthesis and physiological characteristics of radish seedlings under aluminum (Al) stress were studied to provide a reference for ABA to alleviate the mechanism of aluminum stress in vegetables.The results showed that when the dose of ABA was lower than 20 μmol?L-1,ABA treatment relieved Al stress to the growth of radish,and increased the photosynthetic pigment content,net photosynthetic rate(Pn),stomatal conductance(Gs) transpiration rate(Tr),SOD activity,POD activity,CAT activity of radish,which obviously strengthened the photosynthesis and antioxidase enzyme activity of radish.Meanwhile,the MDA content decreased and soluble protein content increased when the dose of ABA was lower than 20 μmol?L-1,which efficiently maintained the balance level of intracellular activated oxygen metabolism.Therefore,ABA soaking seeds could relieved Al stress to radish seedlings,and the best dose of ABA was 5 μmol?L-1.
参考文献/References:
[1]却志群.铝胁迫对萝卜种子萌发和根系生长的影响[J].种子,2012,31(7):103-105. [2]刘强,贺根和,李晓红,等.酸、铝浸种对萝卜种子萌发和芽苗生长的影响[J].种子,2011,30(12):12-14. [3]高志奎,李明.蔬菜栽培学各论[M].北京:中国农业科学技术出版社,2006. [4]CHEN Q,WU K H,ZHANG Y N,et al.Physiological and molecular responses of broad bean ( Vicia faba L.) to aluminum stress[J].Acta Physiologiae Plantarum,2012(34):2251-2263. [5]郑向丽,叶花兰,徐国忠.不同基因型决明属的耐铝特点[J].应用生态学报,2010,21(8):1998-2003. [6]MA J F,RYAN P R.Understanding how plants cope with acid soils[J].Functional Plant Biology,2010,37:3-6. [7]陶龙兴,王熹,黄效林,等.植物生长调节剂在农业中的应用及发展趋势[J].浙江农业学报,2001,13(5):322-326. [8]成仿云,杜秀娟.低温与赤霉素处理对‘凤丹’牡丹种子萌发和幼苗生长的影响[J].园艺学报,2008,35(4):553-558. [9]姜英,彭彦,李志辉,等.多效唑、烯效唑和矮壮素对金钱树的矮化效应[J].园艺学报,2010,37(5):823-828. [10]刘素纯,萧浪涛,廖柏寒,等.水杨酸对铅胁迫下黄瓜幼苗叶片膜脂过氧化的影响[J].生态环境学报,2006,15(1):45-49. [11]杨洪强,接玉玲.以脱落酸为信使的植物逆境信号传递[J].山东农业大学学报(自然科学版),2001,32(4):549-554. [12]李汝佳,李雪梅.水杨酸、脱落酸和过氧化氢对镉胁迫小麦幼苗光合及抗氧化酶活性的影响[J].生态学杂志,2007,26(12):2096-2099. [13]颜宏,赵伟,张志静,等.盐胁迫下ABA预浸种对向日葵幼苗的缓解效应[J].中国农学通报,2013,29(34):139-143. [14]陈思妍,邹华文.脱落酸浸种提高萌发期水稻种子对涝渍胁迫的抗性研究[J].安徽农业科学,2013(2):593-594. [15]柏军华,王克如,初振东,等.叶面积测定方法的比较研究[J].石河子大学学报(自然科学版),2005,23(2):216-218. [16]郝再彬,苍晶,徐仲.植物生理学实验[M].哈尔滨:哈尔滨工业大学出版社,2004. [17]张以顺.植物生理学实验教程[M].北京:高等教育出版社,2009. [18]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006. [19]刘强,龙婉婉,胡萃,等.铝胁迫对油菜种子萌发及和幼苗生长的影响[J].江苏农业科学,2009(3):60-62. [20]周蓉,陈海峰,王贤智,等.不同发育阶段大豆株高和茎粗QTL的动态分析[J].植物遗传资源学报,2010,11(3):349-359. [21]乔琳,盛东风,邓艳.重金属铜、锌、铁、铅污染对白菜幼苗鲜重及叶绿素含量的影响[J].广东农业科学,2010,37(2):34-36. [22]肖祥希,刘星辉,杨宗武,等.铝胁迫对龙眼幼苗光合作用的影响[J].热带作物学报,2005,26(1):63-69. [23]周玲,魏小春,郑群,等.脱落酸与赤霉素对瓜尔豆叶片光合作用及内源激素的影响[J].作物杂志,2010(1):15-20. [24]李汝佳,李雪梅.水杨酸、脱落酸和过氧化氢对镉胁迫小麦幼苗光合及抗氧化酶活性的影响[J].生态学杂志,2007,26(12):2096-2099. [25]潘香梅,郁继华,张国斌,等.脱落酸对碱胁迫下黄瓜幼苗叶片膜脂过氧化及保护酶活性的影响[J].甘肃农业大学学报,2010,45(6):75-78. [26]覃世杰,谭长强,候文娟,等.植物生长调节剂GGR6对中国无忧花生理生化的影响[J].北方园艺,2014(22):72-75. [27]史玉炜,王燕凌,李文兵,等.水分胁迫对刚毛柽柳可溶性蛋白、可溶性糖和脯氨酸含量变化的影响[J].新疆农业大学学报,2007,30(2):5-8. [28]李晓科,张义贤.外源脱落酸对大麦幼苗镉胁迫伤害的影响[J].山西农业科学,2012,40(1):18-20.
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备注/Memo
第一作者简介:练华山(1979-),男,本科,副教授,现主要从事蔬菜和花木的教学及科研等工作。E-mail:49939450@qq.com.