XIONG Bingquan,TIAN Jin,LIAO Xiangjian,et al.Establishment of Tissue Culture and Rapid Propagation System of [J].Northern Horticulture,2018,42(07):72-77.[doi:10.11937/bfyy.20173200]
多肉植物玉扇组培快繁体系的建立
- Title:
- Establishment of Tissue Culture and Rapid Propagation System of
- 文献标志码:
- A
- 摘要:
- 以多肉植物玉扇为试材，研究了外植体选择、培养基激素配比对玉扇组织培快繁的影响，以期优化玉扇的组织培养条件，建立适宜于工厂化育苗的玉扇组培快繁技术体系。结果表明：以玉扇的根颈横切片作为外植体效果最佳，玉扇最佳诱导愈伤组织培养基为MS+1.0 mg?L-1 6-BA+0.1 mg?L-1 NAA；最佳分生茎叶培养基为MS+2.0 mg?L-1 6-BA+0.1 mg?L-1 NAA；最佳生根培养基为MS+0.1 mg?L-1 NAA。
- Abstract:
- Haworthia truncata was taken as test material,effects of selection of explants and proportion of hormone in culture medium of tissue culture of Haworthia truncata were studied.In order to optimize the tissue culture conditions of Haworthia truncata,this test established a tissue culture and rapid propagation system that was suitable for industrial seedling rearing.The transverse section of root neck of Haworthia truncata was the best as the explant in callus induction stage.The best media for each stages were as follows：the best medium for callus induction was MS+1.0 mg?L-1 6-BA+0.1 mg?L-1 NAA,the best medium for inducing stem and leaf was MS+2.0 mg?L-1 6-BA+0.1 mg?L-1 NAA,the best medium for inducting root was MS+0.1 mg?L-1 NAA.
参考文献/References:
[1]铭钰.玉扇：叶面如玉形似扇[J].花木盆景，2011（8）：16-18. [2]翟恒华，李耿，李权生.十二卷属植物的生长环境与光照条件[J].黑龙江农业科学，2012（10）：80-81. [3]夏时云，韩伟，丁亮.一种用于增加十二卷属多肉植物组织繁殖速度的专用培养基及组培方法：CN201510502501.4[P].2015-08-14. [4]李劼，张静，卢涛.玉扇的快速繁殖[J].中国科技信息,2008(12):78. [5]孙涛,李德森.截形十二卷的组织培养与快速繁殖[J].植物生理学通讯,2002,38(6):586. [6]常梅，郑一强.植物组织培养技术[M].沈阳：辽宁民族出版社,2013. [7]仉琨.珍稀多肉植物种质资源组培保存及快速繁殖技术[J].中国园艺文摘,2016,32(7):164-165. [8]郭生虎，朱永兴，关雅静.百合科十二卷属玉露的组培快繁关键技术研究[J].中国农学通报，2016,32（34）：85-89.
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备注/Memo
第一作者简介：熊丙全（1976-），男，四川新都人，硕士，副教授，高级农艺师，现主要从事园艺植物及药用植物栽培技术等研究工作。E-mail：45973177@qq.com.责任作者：邓科君（1977-），女，重庆人，博士，副教授，现主要从事植物生物技术应用等研究工作。E-mail：dengkj@uestc.edu.cn.基金项目：四川省高等职业教育创新发展行动计划园林园艺生产性实训基地建设资助项目（XM2-35）。收稿日期：2017-10-12