ZHANG Yukun,YANG Jing,LIN Guocang,et al.Effects of EMS Mutagenesis on Seed Vigor and Agronomic Characteristics of Cowpea[J].Northern Horticulture,2018,42(08):64-68.[doi:10.11937/bfyy.20173180]
甲基磺酸乙酯处理对豇豆种子活力及农艺性状的影响
- Title:
- Effects of EMS Mutagenesis on Seed Vigor and Agronomic Characteristics of Cowpea
- Keywords:
- cowpea; seed vigor; agronomic characteristics; EMS; mutagenesis; breeding
- 文献标志码:
- A
- 摘要:
- 以豇豆品种“青豇909”为试材,利用甲基磺酸乙酯(EMS)进行种子诱变处理并进行田间栽培试验,研究了EMS不同处理浓度对豇豆种子活力、生育期、农艺性状和经济性状等方面的影响,以期为豇豆EMS突变体库的建立和种质创新提供参考依据。结果表明:EMS不同处理对种子活力影响明显,随着处理浓度的增大,种子活力呈递减趋势;各处理出苗期差异明显,但从全生育期来看,与对照相比差异不明显;各处理间农艺性状和经济性状差异明显,第一开花节位总体比对照降低,0.2%的处理最低,单株分枝数0.1%的处理最多;单株结荚数0.5%的处理最多;0.1%的处理荚长最长,单株干荚总质量最大,平均干荚质量0.2%的处理最高。综合考虑,EMS处理浓度为0.1%时,种子活力、出苗率、荚长、荚粗、单株干荚总质量等方面优于其它处理,可作为筛选优质、高产豇豆新品种的EMS处理浓度。
- Abstract:
- Cowpea varieties ‘Qingjiang 909’ was used as test material,the seeds were induced by EMS and field cultivation experiments were carried out,effects of the EMS treatment on seed vigor,growth period,agronomic characteristics and economics traits were studied,in order to provide reference for the establishment of the mutant library and germplasm innovation.The results showed that effect of the EMS treatment concentration on the seed vigor was obviously,with the increase of treatment concentration,the seed vigor showed decreasing trend;the difference of the treatment on seedling stage was obviously,but the difference of the treatment compared with the contrast was unobviously from the whole growth period;the difference of the treatment on agronomic characteristics and economics traits was obviously,the first flowering node was lower than the contrast,the lowest was 0.2% treatment,the number of branches per plant of the 0.1% treatment was the most,the pod numbers per plant of 0.5% treatment were the most;the longest pod length was 0.1% treatment,the total weight of dry weight per plant of 0.1% treatment was the heaviest,the average dry pod weight of 0.2% treatment was the highest.Taking into consideration various factors,under the condition of the EMS 0.1% treatment concentration,which could be used to filtrate high quality and yield of cowpea new varieties,thus seed vigor,seedling rate,pod length,pod thickness,and total weight of dry pods per plant were superior to other treatments.
参考文献/References:
[1]肖杰,艾星,邓稳桥,等.豇豆主要经济性状的遗传效应及相关分析[J].湖南农业大学学报,2004(2):128-130. [2]朱保葛,路子显,耿玉轩,等.烷化剂EMS诱发花生性状变异的效果及高产突变系的选育[J].中国农业科学,1997,30(6):87-89. [3]马惠平,赵永亮,杨光宇.诱变技术在作物育种中的应用[J].遗传,1998,20(6):41-43. [4]张秀省,张荣涛,曹岚,等.EMS诱变的长春花细胞系突变研究[J].中草药,2004,35(1):1293-1296. [5]安学丽,蔡一林,王久光,等.化学诱变及其在农作物育种上应用[J].核农学报,2003,17(3):239-242. [6]王绍伦,高华媛,刘海龙,等.不同基因型花生EMS诱变条件分析及突变系筛选[J].安徽农业科学,2014,42(33):11778-11781. [7]JOONG H L,SEUNG Y L.Selection of stablemutants from cultured rice anthers treated withethyl methane sulfonic acid[J].Plant Cellissueand Culture,2002,71(2):165-171. [8]吴关庭.我国小麦诱变育种的研究进展[J].国外农学(麦类作物),1995,10(1):31-34. [9]赵永亮,宋同明.玉米化学诱变研究进展[J].华北农学报,1998,12(3):139-145. [10]祝丽英,池书敏,刘志增.甲基磺酸乙酯(EMS)在创造玉米新种质中的应用[J].玉米科学,2000,8(1):19-20. [11]安学丽,王久光,王国强.甲基磺酸乙酯(EMS)对玉米自交系诱变效应的研究[J].玉米科学,2003,11(3):74-84. [12]王培英.人工诱变改良大豆品质的研究[J].核农学报,2000,14(1):21-23. [13]张忠武,杨友伟,孙信成.EMS处理对豇豆种子萌发的生理生化效应及EMS处理半致死剂量研究[J].江汉大学学报,2016,44(4):305-310.
相似文献/References:
[1]张伟艳,顾 绘,韩阳瑞,等.九种百子莲属种子活力及萌发特性的研究[J].北方园艺,2014,38(12):49.
ZHANG Wei-yan,GU Hui,HAN Yang-rui,et al.Study on Nine Kinds of Agapanthus L.Her.Seed Vigor and Germination Characteristics[J].Northern Horticulture,2014,38(08):49.
[2]饶贵珍,鲁红学,王香萍,等.豇豆无公害安全高效生产技术及效益分析[J].北方园艺,2014,38(14):230.
[3]贾利元,郭秀英,崔保伟.黄淮海地区豇豆品种筛选试验[J].北方园艺,2013,37(17):44.
JIA Li-yuan,GUO Xiu-ying,CUI Bao-wei.Screening Test on Cowpea Breeds in Huanghuaihai Region[J].Northern Horticulture,2013,37(08):44.
[4]常文静,张学超,杨明花.伊犁河谷豇豆品种比较试验[J].北方园艺,2013,37(17):46.
CHANG Wen-jing,ZHANG Xue-chao,YANG Ming-hua.Comparative Experiment of Cowpea Varieties in Yili Valley[J].Northern Horticulture,2013,37(08):46.
[5]崔路路,于海霞,李景富,等.磁场处理对结球甘蓝种子活力及萌发进程的影响[J].北方园艺,2014,38(02):20.
CUI Lu- lu,YU Hai- xia,LI Jing- fu,et al.Effect of Magnetic Treatment on Seed Vigor and Seed Germination of Cabbage[J].Northern Horticulture,2014,38(08):20.
[6]康利平,张禄.水分胁迫对豇豆幼苗光合特性的影响[J].北方园艺,2012,36(08):17.
KANG Li-ping,ZHANG Lu.Effect of Water Stress on Photosynthetic Characteristics of Cowpea Seedlings[J].Northern Horticulture,2012,36(08):17.
[7]孙艳红,贾艳霞,刘海萍,等.常见抗生素对蔬菜种子萌发和活力的影响[J].北方园艺,2012,36(20):29.
SUN Yan-hong,JIA Yan-xia,LIU Hai-ping,et al.Effect of Common Antibiotics on the Sprouting and the Vigor of Three Vegetable Seeds[J].Northern Horticulture,2012,36(08):29.
[8]尹光晶,宋廷宇,吴春燕,等.豇豆ISSR-PCR反应体系的建立[J].北方园艺,2012,36(12):141.
YINGuang-jing,SONGTing-yu,WUChun-yan,et al.EstablishmentofInterSimpleSequenceRepeatPCRReactionSysteminAsparagusBean[J].Northern Horticulture,2012,36(08):141.
[9]崔路路,于海霞,李景富,等.聚乙二醇对老化甘蓝种子活力的影响[J].北方园艺,2013,37(24):31.
CUI Lu-lu,YU Hai-xia,LI Jing-fu,et al.Effect of PEG on Seed Vigor of Aging Cabbage[J].Northern Horticulture,2013,37(08):31.
[10]周娟,邹礼平.壳聚糖对盐胁迫下豇豆幼苗生长发育的影响[J].北方园艺,2014,38(23):30.
ZHOU Juan,ZOU Li-ping.Effect of Chitosan on Seedling Growth of Cowpea Under Salt Stress[J].Northern Horticulture,2014,38(08):30.
备注/Memo
第一作者简介:张瑜琨(1986-),女,本科,农艺师,现主要从事蔬菜育种与栽培等研究工作。E-mail:949947643@qq.com.责任作者:唐勇(1972-),男,本科,高级农艺师,现主要从事作物育种与栽培等研究工作。E-mail:79176863@qq.com.基金项目:新疆农业科学院青年基金资助项目(xjnkq-2016010);乌鲁木齐市科技计划资助项目(Y141210006)。收稿日期:2017-10-30