[1]张浩,郝立华,刘贵巧,等.正交实验优化酸枣黄酮类物质提取工艺及其体外抗氧化作用[J].北方园艺,2018,42(03):130-138.[doi:10.11937/bfyy.20172782]
　ZHANG Hao,HAO Lihua,LIU Guiqiao,et al.Optimization of Extraction Process and in vitro Antioxidant Activities of Flavonoid From Zizyphus jujuba[J].Northern Horticulture,2018,42(03):130-138.[doi:10.11937/bfyy.20172782]

点击复制

正交实验优化酸枣黄酮类物质提取工艺及其体外抗氧化作用

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷期数: 2018年03 页码: 130-138 栏目: 出版日期: 2018-02-15

Title:: Optimization of Extraction Process and in vitro Antioxidant Activities of Flavonoid From Zizyphus jujuba

作者:: 张浩1; 2; 郝立华3; 刘贵巧4; 叶嘉1; 2; 郑云普3; 付雨薇1; 1.邯郸学院生命科学与工程学院，河北邯郸 056005；2.河北省高校冀南太行山区资源植物应用技术研发中心，河北邯郸 056005；3.河北工程大学水利水电学院，河北邯郸 056038；4.河北工程大学生命科学与食品工程学院，河北邯郸 056038

Author(s):: ZHANG Hao1; 2; HAO Lihua3; LIU Guiqiao4; YE Jia1; 2; ZHENG Yunpu3; FU Yuwei1; 1.School of Life Science and Engineering,Handan University,Handan,Hebei 056005；2.Research Center for Plant Resources Applied Technology in Jinan Mountain at University of Hebei Province，Handan,Hebei 056005；3.School of Water Conservancy and Hydropower,Hebei University of Engineering,Handan,Hebei 056038；4.School of Life Sciences and Food Engineering,Hebei University of Engineering,Handan,Hebei 056038

关键词:: 正交实验设计; 类黄酮; 抗氧化活性; 酸枣; DPPH?

Keywords:: orthogonal array design; flavonoids; antioxidant activity; Zizyphus jujuba; DPPH?

DOI:: 10.11937/bfyy.20172782

文献标志码:: A

摘要:: 以采集于冀南太行山区的野生酸枣茎、根和叶为试材，采用单因素试验和L₉（3⁴) 正交实验的方法，研究了酸枣不同组织类黄酮提取的最佳工艺及其抗氧化功效，以期优化酸枣不同组织类黄酮提取的工艺参数。结果表明：酸枣茎的最佳提取工艺为乙醇浓度65%、料液比1∶20 g?mL^-1、浸提时间2.5 h、浸提温度60 ℃，且在最佳工艺条件时的类黄酮提取率为10.13%；酸枣根的最佳提取工艺为乙醇浓度65%、料液比1∶10 g?mL^-1、浸提时间2.0 h、浸提温度60 ℃，在此工艺条件时的类黄酮提取率为14.15%；酸枣叶的最佳提取工艺为乙醇浓度65%、料液比1∶25 g?mL^-1、浸提时间3.0 h、浸提温度65 ℃，此最优工艺条件下的类黄酮提取率为18.98%；酸枣茎、根、叶的提取液均具有较好清除DPPH?的能力，且在相同的浸提液浓度下，不同酸枣组织对DPPH?的清除效果为叶>根>茎。

Abstract:: With single factor and orthogonal array design L9 （34) experiments,this study investigated the optimal process parameters for extracting flavonoids from different tissues of Zizyphus jujuba,which was sampled from the Taihang Mountains locates at south of Hebei Province,China.Meanwhile,the scavenging effects of flavonoids on DPPH? were also examined.The results showed that the extraction yield of flavonoids was 10.13% with the optimal parameters that the ethanol concentration,solid-liquid ratio,extraction time,and extraction temperature were 65%,1∶20 g?mL-1,2.5 hours,and 60 ℃ for stems；the extraction yield of flavonoids was 14.15% with the optimal parameters that the ethanol concentration,solid-liquid ratio,extraction time,and extraction temperature were 65%,1∶10 g?mL-1,2.0 hours,and 60 ℃ for roots；the extraction yield of flavonoids was 18.98% with the optimal parameters that the ethanol concentration,solid-to-liquid ratio,extraction time,and extraction temperature were 65%,1∶25 g?mL-1,3.0 hours,and 65 ℃ for leaves；the extractions from the stems,roots,and leaves of Zizyphus jujuba characterized by high antioxidant ability for scavenging the DPPH? with the scavenging ability from high to low was leaves>roots>stems.

参考文献/References:

[1]延玺,刘会青,邹永青,等.黄酮类化合物生理活性及合成研究进展[J].有机化学,2008（9):1534-1544.

[2]许晖,孙兰萍,张斌,等.花生壳黄酮类化合物的研究进展[J].农产品加工,2008（3):11-15.

[3]董晓宁,赵海福,赵强,等.中草药提取物抗氧化作用及其机理研究进展[J].中兽医医药杂志,2014（1):25-29.

[4]陈梅霞,苏洁,张玥莉,等.银杏叶提取物与银杏黄酮对高脂血症大鼠血脂水平影响的比较研究[J].中国新药杂志,2014（7):833-838.

[5]李洪玉,孙静芸.竹叶化学成分研究[J].中药材,2003,26（8):562-563.

[6]罗金岳,陈小燕.从箬竹叶中提取茶多酚的研究[J].林产化工通讯,2003,37（6):15-19.

[7]张英,吴晓琴,俞卓裕.竹叶黄酮和内酯的季节性变化规律研究[J].林业化学与工业,2002（22):65-69.

[8]周荣汉.药用植物化学分类学[M].上海:科学技术出版社,1988:123-141.

[9]黄文,王益.竹叶提取物抑菌特性的研究[J].林产化学与工业,2002（22):68-70.

[10]庞战军,周玖,陈瑗.自由基医学研究方法[M].北京:人民卫生出版社,2000.

[11]CAYUELA M M.Oxygen free radicals and human disease[J].Biochimie,1995,77:147-161.

[12]MARNETT L J.Oxygen radicals,lipid peroxidation and DNA damage[J].Toxicology,2002,181:219-222.

[13]中国植物志编辑委员会.中国植物志（第1卷)[M].北京:科学出版社,1959.

[14]石丽霞,张振家.复方大枣口服液对小鼠免疫机能的影响[J].吉林医药学院学报,2005,26（1):41-42.

[15]苏新医学院.中药人辞典[M].上海:上海人民出版社,上册:397,下册:15-64.

[16]史琪荣,周耘,周萍,等.中药酸枣仁的研究概况[J].药学实践杂志,2004,22（2):94-96.

[17]王晓荣,魏朔男,李薇薇.沙枣叶化学成分的HPLC分析[J].陕西农业科学,2013,41（1):66-68.

[18]李全国,楚杰,王君高,等.乐陵枣叶中总黄酮提取工艺研究[J].中国食物与营养,2013,19（7):51-54.

[19]孙庆雷,王晓,刘建华,等.黄酮类物质抗氧化反应性的构效关系[J].食品科学,2005,26（4):69-73.

[20]李卫,宁正祥.黄酮类化合物的分子修饰[J].食品科学,2005,26（8):505-507.

[21]张德权,台建祥,付勤.生物类黄酮的研究及应用概况[J].食品与发酵工业,1999,25（6):52-57.

[22]焦艳,闻杰,于晓红,等.膜荚黄芪茎叶总黄酮对小鼠细胞免疫功能的影响[J].中国中西医结合杂志,1999,19（6):356-358.

[23]杨学山,祝霞,李颖,等.正交实验优化葡萄酒酵母甘露聚糖提取工艺及其体外抗氧化作用[J].食品科学,2015,36（18):69-74.

[24]王勇.酸枣适应干旱的生理特征和氧化还原特征物质研究[D].北京:中国科学院大学,2015.

[25]董芳.酸枣叶中黄酮类物质的抗旱生理功能研究[D].杨凌:西北农林科技大学,2009.

相似文献/References:

[1]王立江,蒋玥.微波法提取长白山野菊花类黄酮最佳工艺研究[J].北方园艺,2013,37(13):159.
　WANG Li-jiang,JIANG Yue.Study on Optimum Extraction Technology of Total Flavonoid from Chrysanthemum indicum L.Flower in Changbai Mountain by Microwave[J].Northern Horticulture,2013,37(03):159.
[2]余蕾.微波法提取甘草中类黄酮的工艺研究[J].北方园艺,2014,38(07):130.
　YU Lei.Research on Flavonoid Extraction From Glycyrrhiza uralensis Fisch by Microwave Method[J].Northern Horticulture,2014,38(03):130.
[3]王立江.超声波法提取长白山野菊花中类黄酮的工艺研究[J].北方园艺,2013,37(11):133.
　WANG Li-jiang.Study on Ultrasonic Extraction Technology of Flavonoids from Chrysanthemum indicum Flower of Changbai Mountain[J].Northern Horticulture,2013,37(03):133.
[4]张传丽,陈鹏.银杏类黄酮研究进展[J].北方园艺,2014,38(03):177.
　ZHANG Chuan-li,CHEN Peng.Research Progresses on Flavonoid of Ginkgo biloba[J].Northern Horticulture,2014,38(03):177.
[5]冯博,李晓东,杨利,等.萱草属植物蚜虫密度及其与类黄酮抗蚜性关系研究[J].北方园艺,2013,37(19):124.
　FENG Bo,LI Xiao-dong,YANG Li,et al.Study on Aphid Density and the Relationship Between Flavonoids and Aphid Resistance of Hemerocallis L.[J].Northern Horticulture,2013,37(03):124.
[6]张俊环,杨丽,孙浩元,等.不同品种杏果实发育进程中多酚与类黄酮物质含量的变化[J].北方园艺,2012,36(24):1.
　ZHANG Jun-huan,YANG Li,SUN Hao-yuan,et al.Changes of the Total Phenols and Flavonoids in Apricot Peel and Pulp of Different Cultivars During Fruit Development[J].Northern Horticulture,2012,36(03):1.
[7]宋伊真,张玉刚,祝军,等.“鲁加”系列苹果果实发育期酚类和果皮色素含量变化研究[J].北方园艺,2014,38(24):28.
　SONG Yi-zhen,ZHANG Yu-gang,ZHU Jun,et al.Study on Change of Phenols and Pericorp Pigment Content During Growth Period of ‘Lujia’ Apple Series[J].Northern Horticulture,2014,38(03):28.
[8]穆红梅,杜秀菊,张秀省,等.植物MYB转录因子调控苯丙烷类生物合成研究[J].北方园艺,2015,39(24):171.[doi:10.11937/bfyy.201524046]
　MU Hongmei,DU Xiuju,ZHANG Xiusheng,et al.Study on Plants MYB Transcription Factors Regulate Biological Synthesis of Phenylpropanoid Metabolism[J].Northern Horticulture,2015,39(03):171.[doi:10.11937/bfyy.201524046]
[9]郭欣慰.,黄丛林,吴忠义,等.植物类黄酮生物合成的分子调控[J].北方园艺,2011,35(04):0.[doi:10.11937/bfyy.201104075]
　GUO Xin-wei.,HUANG Cong-lin,WU Zhong-yi,et al.Molecular Regulation of Plant Fiavonoid Biosynthesis Pathway[J].Northern Horticulture,2011,35(03):0.[doi:10.11937/bfyy.201104075]
[10]李佳,张娜,赖晓辉,等.两种独行菜种子cDNA-SRAP反应体系的建立及优化[J].北方园艺,2016,40(24):99.[doi:10.11937/bfyy.201624025]
　LI Jia,ZHANG Na,LAI Xiaohui,et al.Establishment and Optimization of cDNA-SRAP Reaction System by Orthogonal Design for Two Species of Lepidium L.[J].Northern Horticulture,2016,40(03):99.[doi:10.11937/bfyy.201624025]
[11]张浩,叶嘉,郝立华,等.正交实验优化卷柏类黄酮提取工艺及其体外抗氧化作用[J].北方园艺,2017,41(21):134.[doi:10.11937/bfyy.20171938]
　ZHANG Hao,YE Jia,HAO Lihua,et al.Optimization of Extraction Process and in vitro Antioxidant Activities of Flavonoid From Selaginella tamariscina[J].Northern Horticulture,2017,41(03):134.[doi:10.11937/bfyy.20171938]

备注/Memo

第一作者简介：张浩（1983-），男，硕士，讲师，研究方向为系统与进化植物学。E-mail：zhanghao_55@163.com.

责任作者：郑云普（1981-），男，博士，副教授，研究方向为植物生理生态学。E-mail：zhengyunpu_000@sina.com.

基金项目：国家自然科学基金青年基金资助项目（31400418）；河北省科技计划资助项目（12272502）；邯郸市科学技术研究与发展计划资助项目（1323108093-9）；河北省自然科学基金青年基金资助项目（C2014109013）；河北省科技支撑资助项目（11230607D-5）；河北省高等学校科学技术研究资助项目（ZD2017302）；邯郸学院校级科研资助项目（15217，15105）。

收稿日期：2017-10-12

更新日期/Last Update: 2018-02-09