XIAO Min,ZENG Xiangping,YAN Wanrong,et al.Toxicity Test of Eight Fungicides to Chiehqua Colletotrichum and Control Effect in Fields[J].Northern Horticulture,2017,41(23):16-22.[doi:10.11937/bfyy.20172394]
八种药剂对节瓜炭疽病菌的毒力测定与田间防效
- Title:
- Toxicity Test of Eight Fungicides to Chiehqua Colletotrichum and Control Effect in Fields
- Keywords:
- Chiehqua Colletotrichum; isolation and identification; phylogenetic analysis; indoor toxicity; field control effect
- 文献标志码:
- A
- 摘要:
- 以节瓜品种 “粤农”为试材，以节瓜炭疽病菌为防治对象，采用菌丝生长速率及农药田间药效试验方法，研究了8种药剂对节瓜炭疽病菌的室内毒力和田间防效，以期为海南节瓜炭疽病的防治提供参考依据。结果表明：代表菌株J-56与Colletotrichum gloeosporioides strain JX-1（HQ645079.1），Colletotrichum gloeosporioides isolate C16（KC010547.1）的ITS序列聚在同一分支上，同源性达99%，属于胶孢炭疽菌Colletotrichum gloeosporioides。室内毒力测定中，50%咪鲜胺锰盐WP和250 g?L-1嘧菌酯SC毒力较高，EC50分别为0.000 9、0.012 7 μg?mL-1，其次为43%戊唑醇SC、40%多?福?溴菌腈WP、80%代森锰锌WP、70%甲基托布津WP和10%苯醚甲环唑WG，20%嘧霉胺WP的毒力最低，EC50为60.511 9 μg?mL-1。大田试验中，43%戊唑醇SC和50%咪鲜胺锰盐WP 2个处理防效较高，为80.64%和78.16%,但与10%苯醚甲环唑WG、40%多?福?溴菌腈WP和250 g?L-1嘧菌酯SC 5个处理间防效相当，差异不显著；20%嘧霉胺WP处理的防效最低。结合室内毒力和大田试验，生产上可使用43%戊唑醇SC、50%咪鲜胺盐锰WP、10%苯醚甲环唑WG、40%多?福?溴菌腈WP和250 g?L-1嘧菌酯SC 5种药剂防治节瓜炭疽病。
- Abstract:
- ‘Yuenong’ Chiehqua was used as material,and the Chiehqua Colletotrichum was used as control object.Via the methods of pathogens mycelium growth rate and field trials of fungicides,the indoor toxicity and the control effect of 8 kinds of fungicides on the field anthracnose were studied,so as to provide reference for the prevention and control of melon anthracnose.The results showed that all of the ITS sequence which included Colletotrichum gloeosporioides strain JX-1(HQ645079.1),J-56 strain,Colletotrichum gloeosporioides isolate C16(KC010547.1) located in the same branch with sequence homology of 99%.It belonged to Colletotrichum gloeosporioides.50% prochloraz manganese WP and azoxystrobin SC of 250 g?L-1 were highly toxic to this strain with EC50 value of 0.000 9 μg?mL-1 and 0.012 7 μg?mL-1,followed by 43% tebuconazole SC,40% carbendazim thiram bromothalonil WP,80% carmazine WP,70% thiophanate methyl WP,10% difenoconazole WG.20% pyrimethanil WP had a little toxicity with EC50 value of 60.511 9 μg?mL-1.The field experiment results showed that 43% tebuconazole SC and 50% prochloraz manganese WP had good control efficiency of 80.64% and 78.16%.But treatments of 43% tebuconazole SC,50% prochloraz manganese WP,10% difenoconazole WG,40% carbendazim thiram bromothalonil WP and azoxystrobin SC of 250 g?L-1 were fairly between each other,no significant difference.Treatment of 20% pyrimethanil WP had minimum control effect.According to indoor toxicity tests and field trails,43% tebuconazole SC,50% prochloraz manganese WP,10% difenoconazole WG,40% carbendazim thiram bromothalonil WP and azoxystrobin SC of 250 g?L-1 could be used to control Chiehqua Colletotrichum in turn in practical production.
参考文献/References:
[1]徐红梅，陈京元，肖德林.林木炭疽病研究进展[J].湖北林业科技,2004（4):40-42.
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备注/Memo
第一作者简介：肖敏（1970-），女，硕士，副研究员，现主要从事植物病害综合防治等研究工作。E-mail：xiaominnky@21cn.com.