[1]李泽宏,冯如,袁红慧,等.银杏叶片中萜内酯的提取工艺[J].北方园艺,2017,41(23):176-180.[doi:10.11937/bfyy.20172299]
　LI Zehong,FENG Ru,YUAN Honghui,et al.Extraction Process for Components of Ginkgo biloba Ginkgolides and Bilobalide[J].Northern Horticulture,2017,41(23):176-180.[doi:10.11937/bfyy.20172299]
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银杏叶片中萜内酯的提取工艺

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年23 页码: 176-180 栏目: 出版日期: 2017-12-10
Title:
Extraction Process for Components of Ginkgo biloba Ginkgolides and Bilobalide
作者:
李泽宏; 冯如; 袁红慧; 程华; 程水源
武汉轻工大学 生物与制药工程学院，湖北 武汉 430023
Author(s):
LI Zehong; FENG Ru; YUAN Honghui; CHENG Hua; CHENG Shuiyuan
School of Biology and Pharmaceutical Engineering，Wuhan Polytechnic University，Wuhan,Hubei 430023
关键词:
银杏叶片; 萜内酯; 提取工艺
Keywords:
Ginkgo biloba leaves; ginkgolides and bilobalide; extraction process
DOI:
10.11937/bfyy.20172299
文献标志码:
A
摘要:
以银杏叶片为试材，采用乙醇-水浸提法，设计单因素试验，研究了乙醇浓度、提取时间、料液比、提取温度4个提取参数对萜内酯得率的影响，优化了提取工艺。结果表明：提取萜内酯的最佳工艺为料液比1∶150 g?mL-1，提取温度60 ℃，60%乙醇，提取2.0 h；最佳洗脱条件是石油醚∶乙酸乙酯=1∶2（v/v），一次性洗脱。经过HPLC-ELSD分析，该工艺提取率高，操作简便，具有可信度。
Abstract:
The effects of four extraction parameters on the ginkgolides and bilobalide yield were optimized by taking Ginkgo biloba leaves as material，using ethanol-water extraction，single factor test.The ethanol concentration，extraction time，the ratio of solid-liquid and extraction temperature on the content of ginkgolides and bilobalide were studied.The extraction process was optimized.The results showed that the optimal extraction conditions were as follows,under the condition of the ratio of solid-liquid 1∶150 g?mL-1，extraction temperature 60 ℃，extracting 2.0 hours with 60% ethanol.Petroleum ether∶ethyl acetate =2∶1(v/v).By the analysis of HPLC-ELSD，the extraction rate was high，simple，with credibility.

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备注/Memo

第一作者简介：李泽宏（1992-），男，硕士研究生，研究方向为药物化学。E-mail：lee_zeno@foxmail.com.

责任作者：程水源（1965-），男，教授，博士生导师，研究方向为经济林种质资源评价与利用。E-mail：s_y_cheng@sina.com.
基金项目：国家自然科学基金资助项目（31270717，31400556）；湖北省自然科学基金创新群体资助项目（2011CDA117）。
收稿日期：2017-07-10

更新日期/Last Update: 2017-12-20