[1]吕佳恒,管雪强,孙玉霞,等.摘叶处理对“赤霞珠”葡萄3-异丁基-2-甲氧基吡嗪积累的影响[J].北方园艺,2017,41(11):23-28.[doi:10.11937/bfyy.201711005]
　LYU Jiaheng,GUAN Xueqiang,SUN Yuxia,et al.Effects of Leaf Removal on 3-Isobutyl-2-Methoxypyrazine Accumulation in ‘Cabernet Sauvignon’ Grape[J].Northern Horticulture,2017,41(11):23-28.[doi:10.11937/bfyy.201711005]

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摘叶处理对“赤霞珠”葡萄3-异丁基-2-甲氧基吡嗪积累的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷期数: 2017年11 页码: 23-28 栏目: 出版日期: 2017-06-15

Title:: Effects of Leaf Removal on 3-Isobutyl-2-Methoxypyrazine  Accumulation in ‘Cabernet Sauvignon’ Grape

作者:: 吕佳恒1; 2; 3; 管雪强2; 3; 孙玉霞2; 3; 王世平2; 3; 王恒振2; 3; （1.山东农业大学食品科学与工程学院，山东泰安 271018；2.山东省农产品精深加工技术重点实验室，山东济南 250100； 3.山东省农业科学院农产品研究所，山东济南 250100）

Author(s):: LYU Jiaheng1; 2; 3; GUAN Xueqiang2; 3; SUN Yuxia2; 3; WANG Shiping2; 3; WANG Hengzhen2; 3; (1.College of Food Science and Engineering，Shandong Agricultural University，Tai′an，Shandong 271018;2.Key Laboratory of Agro-products Processing Technology of Shandong，Jinan，Shandong 250100;3.Institute of Agro-food Science and Technology，Shandong Academy of Agricultural Science，Jinan，Shandong 250100)

关键词:: 摘叶; “赤霞珠”葡萄; 3-异丁基-2-甲氧基吡嗪; 顶空固相微萃取 

Keywords:: leaf removal; ‘Cabernet Sauvignon’ grape; IBMP; HS-SPME

DOI:: 10.11937/bfyy.201711005

文献标志码:: A

摘要:: 以5年生“赤霞珠”葡萄为试材，通过果穗周围叶片全部摘除的方式，采用顶空固相微萃取（HS-SPME）结合气质联用（GC-MS）技术，利用稳定同位素做内标来检测葡萄果实中3-异丁基-2-甲氧基吡嗪（IBMP）的含量，确定最佳摘叶处理的时间以有效降低烟台“赤霞珠”葡萄中IBMP的含量。结果表明：花前摘叶处理可以明显降低葡萄果实中IBMP的含量，而转色期摘叶不会影响葡萄果实中IBMP的含量；HS-SPME结合GC-MS，利用稳定同位素做内标的方法具有较低的检测限和定量限。花前摘叶处理能有效降低“赤霞珠”葡萄IBMP的含量，进而可以改善胶东“赤霞珠”葡萄酒香气。

Abstract:: Five-year-old ‘Cabernet Sauvignon’ grapes were selected as experimental materials.All around berry leaves were removed in early flowering and veraison period respectively.Headspace solid phase microextraction (HS-SPME) with stable isotope dilution gas chromatography/mass spectrometry (GC-MS) method for IBMP quantification in grapes was very efficient.The objective was to changing the light intensity to reduce IBMP concentration in grapes leaf removal.Determined the best time of leaf removal which could effectively reduce IBMP content in ‘Cabernet Sauvignon’ grape from Yantai.The results showed that，the limit of detection (LOD) and limit of quantitation (LOQ) were very low in model juice and samples.Leaf removal in preflowering (LR-pre) had significant effect on the accumulation of IBMP during the growth of ‘Cabernet Sauvignon’ grapes，while the leaf removal in veraison (LR-v) had no effect on concentration in harvest.The method of quantitative analysis of IBMP had low detection line and high recovery rate.Earlier leaf removal treatment could reduce the accumulation of IBMP in grape fruit more effectively in preflowering，thus reduce the ‘green pepper’ odor in ‘Cabernet Sauvignon’ wine.

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备注/Memo

第一作者简介：吕佳恒（1992-），男，硕士研究生，研究方向为微域环境对葡萄与葡萄酒的影响。E-mail：15605451493@163.com.责任作者：王恒振（1978-），男，硕士，助理研究员，现主要从事葡萄栽培技术与生理等研究工作。E-mail：chinajinan@163.com.基金项目：山东省农业科学院青年基金资助项目(2016YQN50)；泰山学者特聘专家资助项目;山东省农业重大应用技术创新课题资助项目。

更新日期/Last Update: 2017-06-12