LI Xiaowei,ZHANG Min,SHEN Lijun,et al.Analysis of Content of Cordycepin in Liquid Fermentation of Cordyceps militaris[J].Northern Horticulture,2017,41(19):170-173.[doi:10.11937/bfyy.20170907]
蛹虫草液体发酵过程中虫草素含量的分析
- Title:
- Analysis of Content of Cordycepin in Liquid Fermentation of Cordyceps militaris
- Keywords:
- Cordyceps militaris; liquid fermentation; cordycepin; HPLC; yeild
- 文献标志码:
- A
- 摘要:
- 以2个蛹虫草菌株2014072503-1和2014072301-1为试材，采用高效液相色谱（HPLC）法，分析蛹虫草40 d液体发酵过程中其发酵液中的虫草素产量，研究了蛹虫草发酵时间对虫草素产量的影响，旨在确定虫草素开始产生、产量迅速升高、产量最高的3个关键时间点，为下一步对以上时间节点的菌丝样品进行转录组测序分析，进而挖掘与虫草素产量密切相关的候选基因奠定基础。结果表明：2个蛹虫草菌株发酵过程中产虫草素的起始时间点为发酵后3 d，迅速升高的时间点是12 d，虫草素产量最高的时间点2014072503-1号菌株为发酵后37 d，而2014072301-1号菌株为发酵后34 d。2014072301-1号菌株发酵产生的虫草素含量高（382.43 μg?mL-1），时间短（34 d），更适于作为生产虫草素的菌株。
- Abstract:
- The yield of cordycepin in fermentation liquid from Cordyceps militaris strains 2014072503-1 and 2014072301-1 during 40 days by HPLC were determined.The effects of fermentation time of Cordyceps militaris on yield of cordycepin to find the key point time of cordycepin producing were investigated，and this would be laid a foundation for further research of transcriptome sequencing and of functional identification about cordycepin synthesis related genes.The results showed that the three key point time of cordycepin synthesis in submerged culture during 40 days were 3 days，12 days，37 days for 2014072503-1 strain and 3 days，12 days，34 days for 2014072301-1 strain，respectively.The maximal amount of cordycepin reached 382.43 μg?mL-1 of 2014072301-1 strain，so it might be a suitable strain to produce cordycepin in the future.
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备注/Memo
第一作者简介：李晓薇（1981-），女，博士，讲师，研究方向为菌物生物反应器的开发与利用。E-mail：xiaoweili1206@163.com.