WANG Renhan,XIANG Yuanping,WANG Hui.SCAR Marker Linked to Onion Male Sterile Gene and Its Application[J].Northern Horticulture,2017,41(02):95-98.[doi:10.11937/bfyy.201702024]
与洋葱雄性不育基因紧密连锁的SCAR标记及其应用
- Title:
- SCAR Marker Linked to Onion Male Sterile Gene and Its Application
- Keywords:
- Onion(Allium cepa L.); male sterile line; molecular marker
- 文献标志码:
- A
- 摘要:
- 以10份已知育性的洋葱材料和20份未知育性的洋葱后代材料为试材，采用SCAR标记技术，研究了标记OC2175在洋葱雄性不育系和保持系中的扩增情况，以验证该标记在洋葱细胞质雄性不育系选择方面的应用前景。结果表明：该标记可在不育系统扩增出2 175 bp和1 053 bp的2条片段，而在相应的保持系中仅扩增出1 053 bp的特异片段。随后对20份未知育性的后代材料进行了PCR检测，结果显示有4份材料为可育株，另外16份材料为不育株。进一步对这20份未知育性的洋葱后代材料进行田间检测，发现分子鉴定为可育株的材料中有1株在田间表现为不育。可见，分子鉴定与田间鉴定的吻合度为95%，该标记可用于洋葱细胞质雄性不育株的分子标记辅助筛选。
- Abstract:
- Five onion male sterile lines，five maintainer lines and 20 unknown fertility progeny materials were used as main test materials.The amplification of SCAR marker OC2175 in onion male sterile lines and corresponding maintainer lines were studied.The results showed that the marker could amplify two fragments of 2 175 bp and 1 053 bp in all male sterile materials，and the corresponding maintainer lines were only amplified specific fragment of 1 053 bp.Further 20 unknown fertility materials were detected using SCAR marker OC2175，and the result indicated that 4 materials was sterile，the other 16 materials were fertility plants.The field investigation was carried out on the materials of the 20 unknown onion progeny plant，and one plant in direct contradiction to that of molecular identification.The accuracy rate of molecular identification was 95%，and the marker OC2175 could be used for the screening of the molecular marker assisted selection of the onion fine male sterile line.
参考文献/References:
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备注/Memo
第一作者简介：王仁汉（1989-），男，山东菏泽人，硕士研究生，研究方向为蔬菜遗传育种及生物技术。E-mail：245262088@qq.com.责任作者：王辉（1981-），男，山东菏泽人，博士，讲师，研究方向为蔬菜遗传育种及生物技术。E-mail：fromstick@163.com.基金项目：山东省良种工程资助项目（669-1314194）；青岛农业大学高层次人才科研基金资助项目（663-1115041）；山东省自然科学基金资助项目（ZR2014CQ034）。