HAN Weibo,LIU Li,LI Yuyao,et al.Cytological Effects of Amaranthus hypochondriacus L.Irradiated by Different Mutation Treatments[J].Northern Horticulture,2017,41(14):85-88.[doi:10.11937/bfyy.20170021]
不同诱变处理下籽粒苋种子细胞学效应的比较
- Title:
- Cytological Effects of Amaranthus hypochondriacus L.Irradiated by Different Mutation Treatments
- 文献标志码:
- A
- 摘要:
- 以籽粒苋种子为试材，采用搭载第18颗返回式卫星和60Co-γ射线2种方法诱变处理籽粒苋种子，研究了种子根尖细胞学效应。结果表明：太空诱变对籽粒苋的根尖细胞有丝分裂活动无明显影响，γ射线辐照抑制了籽粒苋根尖细胞的有丝分裂，根尖细胞有丝分裂指数并随着γ射线剂量的增加逐渐降低。经卫星搭载和γ射线辐照后，籽粒苋种子根尖细胞均出现了包括微核、桥、落后染色体、游离染色体、粘连染色体等染色体畸变类型，单微核是主要畸变类型。第18颗返回式卫星搭载的辐射剂量相当于60Co-γ射线辐照20~30 Gy。
- Abstract:
- Dry seeds of Amaranthus hypochondriacus L.was used as material,and irradiated by aerospace environment and 60Co-γ-ray to study focus on cytological effects in root tip cell.The results showed that mitotic index irradiated by aerospace environment was not distinct difference than control.The mitotic index irradiated by 60Co-γ-ray was lower than control，with the increase of 60Co-γ-rays dosage;the mitotic index was more lower than control.Seeds irradiated by aerospace environment and 60Co-γ-rays，there were different aberrations in root tip cells，such as micronucleus,chromosome bridge,dissociative chromosome,lagging chromosome and chromosome conglutination.The dosage of aerospace environment on 18th recoverable satellite was similar to 60Co-γ-ray between 20 Gy and 30 Gy.
参考文献/References:
[1]刘录祥,郭会君,赵林姝,等.植物诱发突变技术育种研究现状与展望[J].核农学报,2009,23(6):1001-1007. [2]刘录祥,郭会君,赵林姝,等.我国作物航天育种20年的基本成就与展望[J].核农学报,2006,21(6):589-592. [3]韩微波,张月学,唐凤兰,等.我国牧草诱变育种研究进展[J].核农学报,2010,24(1):62-66. [4]李懋学,张赞平.植物染得体研究技术[M].哈尔滨:东北林业大学出版社,1991. [5]沈光平,王钦南,周祉祯.微核与染色体畸变的相关性[J].遗传,1985,7(1):15-17. [6]刘录祥,韩微波,郭会君,等.高能混合粒子场诱变小麦的细胞学效应研究[J].核农学报,2005,19(5):327-331. [7]刘丽，唐凤兰，张月学,等.空间飞行和60Co-γ射线辐照对苦荬菜种子的细胞学效应研究[J].草地学报,2007,15(5):469-472. [8]张月学,刘杰淋,韩微波,等.空间环境对紫花苜蓿的生物学效应[J].核农学报,2009,23(2):266-269. [9]韩微波.我国苜蓿空间环境诱变育种研究进展及展望[J].核农学报,2014,28(8):1379-1383. [10]杨垂绪[美].太空放射生物学[M].广州:中山大学出版社,1995:65-66,206. [11]李谨,耿金鹏,曹天光,等.太空诱变育种的研究进展[J].北方园艺,2015(14):189-193.
相似文献/References:
[1]孙利娜,王华新,龚建英,等.宝巾花辐射诱变效应研究初报[J].北方园艺,2013,37(19):91.
SUN Li-na,WANG Hua-xin,GONG Jian-ying,et al.Preliminary Study on the Mutagenic Effects of Radiation on Bougainvillea glabra[J].Northern Horticulture,2013,37(14):91.
[2]李谨,耿金鹏,曹天光,等.太空诱变育种的研究进展[J].北方园艺,2015,39(14):189.[doi:10.11937/bfyy.201514048]
LI Jin,GENG Jinpeng,CAO Tianguang,et al.Research Progress on Space Mutation Breeding[J].Northern Horticulture,2015,39(14):189.[doi:10.11937/bfyy.201514048]
[3]王菲,宋冰,李丹,等.香菇60Co-γ辐照突变菌株的筛选[J].北方园艺,2017,41(15):151.[doi:10.11937/bfyy.20170775]
WANG Fei,SONG Bing,LI Dan,et al.Preliminary Screening of Lentinula edodes Mutant Strains by 60Co-γ Irradiation[J].Northern Horticulture,2017,41(14):151.[doi:10.11937/bfyy.20170775]
[4]丁兰,耿金鹏,秦垒,等.质子和太空辐射对金鱼草的诱变效应[J].北方园艺,2019,43(15):82.[doi:10.11937/bfyy.20184196]
DING Lan,GENG Jinpeng,QIN Lei,et al.Mutagenic Effects of Proton and Space Radiation on Antirrhinum majus[J].Northern Horticulture,2019,43(14):82.[doi:10.11937/bfyy.20184196]
[5]李亚洁,孟楠,温志新,等.60Co-γ射线与ARTP复合诱变选育蛹虫草高产菌株[J].北方园艺,2019,43(20):112.[doi:10.11937/bfyy.20190993]
LI Yajie,MENG Nan,WEN Zhixin,et al.Breeding of High Yield Strains of Cordyceps militaris by Combined Mutation of 60Co-γ and ARTP[J].Northern Horticulture,2019,43(14):112.[doi:10.11937/bfyy.20190993]
备注/Memo
第一作者简介：韩微波（1979-），男，陕西兴平人，硕士，副研究员，现主要从事牧草种质资源创新与诱变育种等研究工作。E-mail：alclever@163.com.基金项目：中央引导地方科技发展专项资助项目（ZY16C05）；优质饲料作物育繁推一体化资助项目。