JIANG Tong,WANG Hui,YANG Yanjie,et al.Selection of InDel Markers Associated With Male Sterile Gene in Dry Pepper[J].Northern Horticulture,2017,41(15):24-28.[doi:10.11937/bfyy.20165158]
干制辣椒雄性不育系与保持系间多态性 InDel标记的筛选
- Title:
- Selection of InDel Markers Associated With Male Sterile Gene in Dry Pepper
- Keywords:
- dry pepper; male sterile; InDel marker
- 文献标志码:
- A
- 摘要:
- 以3对干制辣椒雄性不育系以及相应保持系（15005A，15006B；16011A，16012B；16063A，16064B）为试材，采用InDel标记的方法对干制辣椒雄性不育系与保持系间进行检测，以期筛选出干制辣椒雄性不育与保持系间存在多态性的标记。结果表明：在44个InDel标记中，仅有一个标记CIDH776在3对不育系和保持系间存在一致的多态性，该标记在不育系中分别扩增出约为600 bp和650 bp左右的DNA片段,而在相应保持系中均未扩增出相应条带。初步认为，分子标记CIDH776可用于干制辣椒雄性不育系与保持系间的分子标记辅助鉴定。
- Abstract:
- Three pairs of dry pepper male sterile lines and maintain lines (15005A，15006B；16011A，16012B；16063A，16064B) were used as materials，and polymorphism InDel markers was selected between sterile lines and corresponding maintainer lines.The results indicated that only one marker CIDH776 in the 44 InDel markers showed the same polymorphism between three pair of male sterile lines and corresponding maintainer lines.Two DNA fragments about 600 bp and 650 bp were amplified in three male sterile lines，but there was no amplification in corresponding maintainer lines.Therefore，InDel marker CIDH776 could be used for molecular marker assisted identification of male sterile line and maintainer line.
参考文献/References:
[1]傅鸿妃，柴伟国，吕晓菡,等.雄性不育在我国辣椒品种选育中的应用及不育基因定位研究进展[J].现代农业科技,2016(3):119-121. [2]张志刚，赵智中，李巧云,等.大白菜InDels标记开发及其在剩余杂合体鉴定中的应用[J].农业生物技术学报，2016，24(4)：510-518. [3]姚国新,黄文超.利用水稻籼粳分化InDel标记鉴定育种材料的籼粳属性[J].杂交水稻,2013,28(3):53-57. [4]葛敏,蒋璐,张晓林,等.利用Insertion/Deletion(InDel)分子标记检测玉米互交种混杂的原理及应用[J].分子植物育种,2013,11(1):37-47. [5]申璐,沈炎林,柴敏,等.采用InDel和SSR标记分析番茄品种基因组DNA 多态性[J].中国农业大学学报,2011,16(2)：34-42. [6]李斯更,沈镝,刘博,等.基于黄瓜基因组重测序的InDel标记开发及其应用[J].植物遗传资源学报，2013,14(2)：278-283. [7]LI W，CHENG J，WU Z，et al.An InDel-based linkage map of hot pepper (Capsicum annuum)[J].Molecular Breeding，2015，35(1):1-10. [8]谢立波,郭亚华,孟凡娟,等.辣椒RAPD扩增体系的建立[J].安徽农业科学报,2010,38(34)：19272-19274. [9]王得元,王鸣,郑学勤,等.用RAPD分析辣椒细胞质雄性不育基因[J].核农学报,2005,19(2):99-102. [10]王述彬,刘金兵,潘宝贵,等.辣椒细胞质雄性不育系与其保持系基因组DNA的RAPD分析[J].上海农业学报,2008,24(1)：8-10. [11]魏兵强，王兰兰，陈灵芝,等.辣椒胞质雄性不育基因的分子标记[J].西北农业学报，2010，19(10)：166，168-173.
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备注/Memo
第一作者简介：姜童（1995-），男，硕士研究生，研究方向为蔬菜遗传育种。E-mail：583944745@qq.com.责任作者：林多（1973-），女，博士，教授，研究方向为蔬菜营养生理与品质育种。E-mail：linduo73@163.com.基金项目：山东省农业重大应用技术创新资助项目（682214007）；山东省农业良种工程资助项目（6622315070）；青岛农业大学应用型人才培养特色名校建设工程教学研究资助项目(XJG2013006，XJG2013127)；青岛农业大学高层次人才科研基金资助项目（6631115041）；山东省自然科学基金资助项目（ZR2014CQ034） 收稿日期：2017-04-01