ZHANG Liang,MA Lingyu,WANG Taixia.Effect of Low-dose Gamma Irradiation on Growth and Development of Arabidopsis Plants[J].Northern Horticulture,2016,40(23):28-32.[doi:10.11937/bfyy.201623007]
低剂量60Co-γ辐照刺激拟南芥植株生长的效应
- Title:
- Effect of Low-dose Gamma Irradiation on Growth and Development of Arabidopsis Plants
- 文献标志码:
- A
- 摘要:
- 以拟南芥为试材,以0、50、150 Gy剂量60Co-γ辐照拟南芥种子,研究其对拟南芥植株生长发育的影响。结果表明:与对照相比,50 Gy辐照处理显著增加了拟南芥植株高度、莲座叶长度、角果长度、种子数量以及叶片光合色素含量;150 Gy辐照拟南芥的各项生长和生理指标显著降低,表明高剂量辐照处理抑制了拟南芥植株的生长。说明低剂量的60Co-γ辐照显著促进拟南芥植株的营养生长和生殖生长。
- Abstract:
- Arabidopsis seeds were irradiated with gamma irradiation at the doses of 0,50 and 150 Gy,respectively and the biological effects of gamma irradiation on plant growth and development were investigated.The results showed that 50 Gy gamma irradiation significantly increased Arabidopsis plant height,rosette leaf length,pod length,number of seeds,and content of photosynthetic pigments.While,gamma irradiation at the dose of 150 Gy obviously decreased the growth and physiological parameters,indicating that gamma irradiation at high doses inhibited Arabidopsis plant growth.Taken together,these results indicated that low-dose gamma irradiation significantly promoted the vegetative and reproductive growth in Arabidopsis plants.
参考文献/References:
[1]DAVIES C,HOWARD D,TAM G,et al.Isolation of Arabidopsisthaliana mutants hypersensitive to gamma radiation[J].Molecular and General Genetics,1994,243:660-665. [2]BACHIRI H,DJEBBAR R,DJENADI C.Gamma irradiation effects on some physiological traits of wheat (Triticum aestivum L.) under control and water stress conditions[J].American Journal of Plant Physiology,2014,9(3):103-109. [3]BORZOUEI A,KAFI M,KHAZAEI H,et al.Effect of gamma radiation on germination and physiological aspects of wheat(Triticum aestivum L.) seedlings[J].Pakistan Journal of Botany,2010,42(4):2281-2290. [4]KIM D Y,HONG M J,PARK C S,et al.The effects of chronic radiation of gamma ray on protein expression and oxidative stress in Brachypodium distachyon[J].International Journal of Radiation Biology,2015,91(5):407-419. [5]WI S G,CHUNG B Y,KIM J S,et al.Effects of gamma irradiation on morphological changes and biological responses in plants[J].Micron,2007,38(6):553-564. [6]MARCU D,CRISTEA V,DARABAN L.Dose-dependent effects of gamma radiation on lettuce(Lactuca sativa var.capitata)seedlings[J].International Journal of Radiation Biology,2013,89(3):219-223. [7]AKSHATHA,CHANDRASHEKAR K R,SOMASHEKARAPPA H M,et al.Effect of gamma irradiation on germination,growth,and biochemical parameters of Terminalia arjuna Roxb[J].Radiation Protection and Environment,2013,36(1):38-44. [8]GICQUEL M,TACONNAT L,RENOU J P,et al.Kinetic transcriptomic approach revealed metabolic pathways and genotoxic-related changes implied in the Arabidopsis response to ionizing radiations[J].Plant Science,2012,195(3):106-119. [9]EI-BELTAGI H S,AHMED O K,EI-DESOUKY W.Effect of low doses g-irradiation on oxidative stress and secondary metabolites production of rosemary (Rosmarinus officinalis L.) callusculture[J].Radiation Physics and Chemistry,2011,80(9):968-976. [10]SHEREEN A,ANSARI R,MUMTAZ S,et al.Impact of gamma irradiation induced changes on growth and physiological responses of rice under saline condition[J].Pakistan Journal Botay,2014,41(5):2487-2495. [11]MOUSSA H R.Low dose of gamma irradiation enhanced drought tolerance in soybean[J].Bulgarian Journal of Agricultural Science,2011,17(1):63-72. [12]LIANG Z,FENGXIA Z,WENCAI Q,et al.Irradiation with low-dose gamma ray enhances tolerance to heat stress in Arabidopsis seedlings[J].Ecotoxicology and Environmental Safety,2016,128:181-188. [13]LICHTENTHALER H K.Chlorophyll and carotenoids:Pigments of photosynthetic biomembranes[J].Methods in Enzymology,1987,148C:350-382. [14]WELLBURN A R.The spectral determination of chlorophylls a and b,as well as total carotenoids,using various solvents with spectrophotometers of different resolution[J].Journal of Plant Physiology,1994,144:307-313. [15]WENCAI Q,LIANG Z,WEISEN F,et al.ROS and ABA signaling are involved in the growth stimulation induced by low-dose gamma irradiationin Arabidopsis seedling[J].Appl Biochem Biotechnol,2015,175(3):1490-1506. [16]JAN S,PARWEEN T,SIDDIQI T O.Gamma radiation effects on growth and yield attributes of Psoralea corylifolia L.with reference to enhanced production of psoralen[J].Plant Growth Regulation,2011,64(2):163-171. [17]SINGH B,DATTA P S.Gamma irradiation to improve plant vigour,attributes of wheat[J].Radiation Physics and Chemistry,2010,79(2):139-143. [18]MARCU D,CRISTEA V,DARABAN L.Dose-dependent effects of gamma radiation on lettuce (Lactuca sativa var.capitata) seedlings[J].International Journal of Radiation Biolo Gy,2013,89(3):219-223. [19]HEGAZI A Z,HAMIDELDIN N.The effect of gamma irradiation on enhancement of growth and seed yield of okra(Abelmoschus esculentus(L.)Monech)and associated molecular changes[J].Journal of Horticulture and Forestry,2010,2(3):38-51. [20]KOVALCHUK I,MOLINIER J,YAO Y,et al.Transcriptome analysis reveals fundamental differences in plant response to acute and chronic exposure to ionizing radiation[J].Mutation Research,2007,624(s1-2):101-113. [21]FAN J,SHI M,HUANG J Z,et al.Regulation of photosynthetic performance and antioxidant capacity by 60Co γ-irradiation in Zizania latifolia plants[J].Journal of Environmental Radioactivity,2014,129(129C):33-42.
备注/Memo
第一作者简介:张亮(1982-),男,博士,副教授,硕士生导师,现主要从事植物逆境胁迫等研究工作。E-mail:zhangliang@htu.edu.cn.责任作者:王太霞(1964-),女,博士,教授,硕士生导师,现主要从事植物结构学等教学与科研等工作。E-mail:wtaixia@sina.com.基金项目:国家自然科学基金资助项目(31300163,31270225);河南师范大学科研启动基金资助项目(5102099179107)。