GUO Jinli,CHEN Guihua,FAN Li,et al.Dynamic Variation of Energy Metabolism During Aging Process in Strawberry Fruit[J].Northern Horticulture,2016,40(20):18-21.[doi:10.11937/bfyy.201620005]
草莓果实采后衰老过程中能量代谢的变化
- Title:
- Dynamic Variation of Energy Metabolism During Aging Process in Strawberry Fruit
- 文献标志码:
- A
- 摘要:
- 以‘红颜’草莓果实为试材，研究了果实采后衰老过程中线粒体呼吸相关酶活性及能量水平的变化，以了解能量与草莓果实衰老的关系。结果表明：随着草莓果实的衰老，草莓果实线粒体琥珀酸脱氢酶（SDH）、H+-ATPase 和 Ca2+-ATPase活性均明显下降，细胞色素氧化酶（CCO）活性上升；三磷酸腺苷(ATP)含量持续下降，二磷酸腺苷(ADP)和磷酸腺苷(AMP)含量上升；ATP、ADP、AMP 3种能量物质含量差异明显，其中ATP含量远高于ADP和AMP含量，ATP含量代表了草莓果实中的能量水平，且能荷(EC)与ATP协同变化；试验表明，草莓果实衰老与线粒体呼吸相关酶活性下降、线粒体功能下降而导致的能量不足有关。
- Abstract:
- The dynamic variation of mitochondria respiratory metabolism-related enzyme activities and energy level were studied during aging process in ‘Hongyan’ strawberry fruit in order to understand the relationship between energy and aging of strawberry fruit.The results showed that，in storage process of strawberry fruit，the activities of mitochondria respiratory metabolism-related enzyme succinic dehydrogenase(SDH)，H+-ATPase，Ca2+-ATPase decreased obviously，cytochrome oxidase(CCO) activity increased.ATP content decreased continuously，contents of ADP，AMP increased，contents of three energy level were different and ATP content was much higher than contents of ADP and AMP，ATP content was the dominant energy resource in fruit.Energy charge had similar trend with ATP.In conclution，decline of activities of mitochondria respiratory metabolism-related enzyme stated the decline of mitochondria function which resulted in energy deficiency.Energy deficiency played a role in aging of strawberry fruit.
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备注/Memo
第一作者简介：郭金丽（1972-），女，内蒙古呼和浩特人，博士，副教授，硕士生导师，现主要从事果树生理和栽培及植物超微弱发光等研究工作。E-mail：guojinli1111@163.com.责任作者：李连国（1959-），男，辽宁朝阳人，博士，教授，博士研究生导师，现主要从事果树生理生态及栽培等研究工作。E-mail：nmndllg@foxmail.com.基金项目：国家自然科学基金资助项目（31260455）。