YANG Yanwen,JIANG Yuantong,ZHANG Lianxue.Study on the Relationship Between Soil Enzyme Activity and Chemical Composition in Farmland and Deforestation Ginseng[J].Northern Horticulture,2016,40(17):157-162.[doi:10.11937/bfyy.201617038]
农田参和伐林参根际土壤酶指标与化学成分比较
- Title:
- Study on the Relationship Between Soil Enzyme Activity and Chemical Composition in Farmland and Deforestation Ginseng
- 文献标志码:
- A
- 摘要:
- 以不同栽培模式的人参及其根际土壤为研究对象,运用典型相关分析方法,对农田参、伐林参中总皂苷、总多糖、总蛋白和总氨基酸等主要有效成分的积累与土壤过氧化氢酶、脲酶、磷酸酶、蔗糖酶活性的关系进行了分析。结果表明:农田栽培模式下,土壤蔗糖酶活性、磷酸酶活性与人参总皂苷含量、总蛋白质含量和总氨基酸含量呈极显著的负相关关系,过氧化氢酶活性与人参总皂苷含量、人参总多糖含量和总蛋白质含量呈极显著负相关关系;伐林栽培模式下,土壤蔗糖酶、脲酶和磷酸酶活性与人参总皂苷含量和总氨基酸含量呈极显著的负相关关系,土壤过氧化氢酶和磷酸酶活性与人参总皂苷含量、人参总多糖和总蛋白质含量呈极显著的负相关关系。表明这几种酶的活性与人参有效成分之间的关系最密切。结合其它养分因子,综合土壤酶活性指标可以作为评价土壤肥力的一个重要生物活性指标,适当调节土壤酶活性,能够加速土壤中各类物质的循环与转化,增加有效成分的积累,从而提高产量与质量。
- Abstract:
- The canonical correlation between effective components (total ginsenoside,polysaccharide,protein,amino acid) and soil enzyme activity(catalase,urease,phosphatase,sucrase) were investigated in farmland ginseng and deformation ginseng production.The results showed that soil sucrase,phosphatase activity had obvious negative correlation with content of total ginsenoside,protein,amino acid,soil catalase had obvious negative correlation with content of total ginsenoside,polysaccharide,protein in farmland ginseng.Soil sucrase,urease,phosphatase activity had obvious negative correlation with content of total ginsenoside,amino acid,soil catalase,phosphatase activity had obvious negative correlation with content of total ginsenoside,polysaccharide,protein in deformation ginseng.The three kinds enzymes had the closest relations with active ingredient of Panax ginseng.Integral soil enzyme activity could be used as a biological index of soil fertility,which,together with other growth factors.The appropriate enzyme activity could accelerate the circulation and transformation of all kinds of material in the soil,improve effectively components accumulation,thus improve the yield and quality.
参考文献/References:
[1]黎阳,张铁军,刘素香,等.人参化学成分和药理研究进展[J].中草药,2009,40(1):164-166. [2]SHIN B K,KWON S W,PARK J H.Chemical diversity of ginseng saponins from Panax ginseng[J].Journal of Ginseng Research,2015,39(4):287-298. [3]葛尔宁,严建伟,梁炳圻.人参的氨基酸含量[J].浙江中医学院学报,1997,21(3):32-33. [4]SUN Y X.Structure and biological activities of the polysaccharides from the leaves,roots and fruits of Panax ginseng C.A.Meyer:An overview[J].Carbohydrate Polymers,2011,85(3):490-499. [5]吴雪松,叶正良,郭巧生,等.东北不同产地人参及其加工品外观性状及非皂苷类成分比较分析[J].中国中药杂志,2012,37(16):2383-2387. [6]EKENLER M,TABATABAI M A.Beta-glucosaminidase activity as an index of nitrogen ineralization in soils[J].Commun Soil Sci Plan,2004(35):1081-1094. [7]DODOR D E,TABATAI M A.Arylamidase activity as an index of nitrogen mineralization in soils[J].Commun Soil Sci Plan,2007(38):2197-2207. [8]张亚玉,孙海,宋晓霞.农田栽参根区土壤酶活性与土壤养分的关系[J].吉林农业大学学报,2010,32(6):661-665. [9]孙海,张亚玉,孙长伟,等.不同生长模式下人参土壤养分状况与人参皂苷含量的关系[J].西北农业学报,2012,21(8):146-152. [10]谢彩香,索风梅,贾光林,等.人参皂苷与生态因子的相关性[J].生态学报,2011,31(24):7551-7563. [11]程海涛,张亚玉,张连学,等.土壤环境与人参生长关系的研究进展[J].中药材,2011,34(2):313-317. [12]贾光林,黄林芳,索风梅,等.人参药材中人参皂苷与生态因子的相关性及人参生态区划[J].植物生态学报,2012,36(4):302-312. [13]王婷婷,刘双,赵洪颜,等.农田栽参和伐林栽参土壤养分及酶活性比较分析[J].安徽农业科学,2014,42(34):12075-12077. [14]孙海.人参土壤主要养分及酶活性研究[D].北京:中国农业科学院,2010:10-11. [15]程海涛.人参不同生育期土壤养分及重金属含量动态变化研究[D].长春:吉林农业大学,2011:9-11. [16]关松荫.土壤酶及其活性研究法[M].北京:农业出版社,1986:273-339. [17]中华人民共和国药典委员会.中国药典:1部[M].北京:中国医药科技出版社,2010:367-368. [18]杨艳文.人参根中多糖含量变化规律的研究[D].长春:吉林农业大学,2008:13-20. [19]周颖,樊荣,张建逵.人参中可溶性蛋白质含量测定[J].辽宁中医药大学学报,2014,16(8):95-96. [20]葛善欣,史俊卿,岳彬,等.人参根大量营养元素与氨基酸的相关性研究[J].中草药,2010,41(4):639-643. [21]邓小华,周清明,周冀衡,等.烟叶质量评价指标间的典型相关分析[J].中国烟草学报,2011,17(3):17-22. [22]LEE W,PARK S H,LEE S,et al.Increase in antioxidant effect of ginsenoside Re-alanine mixture by Maillard reaction[J].Food Chemistry,2012,135(4):2430-2435. [23]ACCOE F,BOECKX P,BUSSCHAERT J,et al.Gross N transformation rates and net N mineralisation rates related to the C and N contents of soil organic matter fractions in grassland soils of different age[J].Soil Biology and Biochemistry,2004,36(12):2075-2087. [24]COOK B D,ALLAN D L.Dissolved organic carbon in old field soils:Compositional changes during the biodegradation of soil organic matter[J].Soil Biology and Biochemistry,1992,24(6):595-600. [25]陈巍,高文远,贾伟,等.人参属药用植物组织和细胞培养的研究进展[J].中草药,2005,36(4):616-620. [26]明乾良,韩婷,黄芳,等.人参皂苷生物合成途径及其相关酶的研究进展[J].中草药,2010,41(11):1913-1917. [27]CHAPPELL J,WOLF F,PROULX J,et al.Is the reaction catalyzed by 3-Hydroxy-3-Methylglutaryl coenzyme a reductase a rate-limiting step for isoprenoid biosynthesis in plants?〖KG-*4〗[J].Plant Physiol,1995,109(4):1337-1343. [28]蔡荟梅,臧爱香,夏春,等.西洋参产量与土壤肥力.微生物和酶活性关系的研究[J].安徽农业科学,2007,35(26):8267-8268.
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备注/Memo
第一作者简介:杨艳文(1979-),男,吉林通榆人,博士研究生,助理研究员,现主要从事药用植物栽培学等研究工作。E-mail:yanwen_79@163.com.责任作者:张连学(1955-),男,教授,博士生导师,现主要从事药用植物栽培学等研究工作。E-mail:zlxbooksee@163.com.基金项目:国家科技重大专项子课题资助项目(2012ZX09304006);农业部公益性行业科研专项资助项目(201303111)。