GUO Fang,WANG Chao,LIU Haipeng,et al.Effect of Light Quality and Light Intensity on the Chlorophyll Fluorescence Parameters of Red Raspberry Tissue Culture Plantlets[J].Northern Horticulture,2016,40(11):19-23.[doi:10.11937/bfyy.201611005]
光质和光强对红树莓组培苗叶绿素荧光参数的影响
- Title:
- Effect of Light Quality and Light Intensity on the Chlorophyll Fluorescence Parameters of Red Raspberry Tissue Culture Plantlets
- 文献标志码:
- A
- 摘要:
- 为了确立红树莓组培苗生长的适宜光质和光强，以红树莓“海尔特兹”组培苗为试材，研究了不同光质和光强处理下红树莓生根组培苗的叶绿素含量及叶绿素荧光参数。结果表明：光质处理中，除R/B（红光∶蓝光=1∶1）处理外，Chl a（叶绿素a）、Chl b（叶绿素b）和Ct（叶绿素总含量）均随着R/B比率的增加呈现先增加后减小的趋势，Chl a和Ct均在2R/B（红光∶蓝光=2∶1）处理下呈现最大值，分别为1.344、1.716 mg?g-1，Chl b则在R/2B（红光∶蓝光=1∶2）处理最高，为0.423 mg?g-1，Chl a、Chl b和Ct均以 10R/B（红光∶蓝光=10∶1）处理最低，分别为0.130、0.033、0.163 mg?g-1，均显著低于其它光质；Chl a/b（叶绿素a/b）在10R/B、2R/B和R/B处理下较高，分别为3.942、3.709、3.653，呈现阳生植物的特性，而在6R/B（红光∶蓝光=6∶1）、W（白光）、4R/B（红光∶蓝光=4∶1）等处理下较低，分别为2.729、2.730、2.747，呈现阴生植物的特性；在R/2B处理下，红树莓组培苗叶片的叶绿素荧光参数值表现极优，Fv/Fm（PSⅡ最大光化学效率）值达到0.881，而10R/B处理下，Fv/Fm值降低到0.355，且显著低于其它光质处理；光强处理中，随着光强的增加，Chl a、Chl b和Ct呈现递减的趋势，最大值由1.218、0.503、1.720 mg?g-1分别降低到0.907、0.302、1.209 mg?g-1，均形成显著差异，Chl a/b则表现为随着光强的增加而增加，在2 000 lx处理达到最大值，为3.042，且3种光强处理差异显著；叶片的Fo（初始荧光）随着光强的增加而增加，在2 000 lx处理下达到最大值，为180.000，Fm（最大荧光值）则在1 500 lx处理下达到最大值，为907.000，而叶片的Fv/Fm、Fv/Fo（PSⅡ的潜在活性）、ΦPSⅡ（PSⅡ实际光化学效率）、Rfd（可变荧光下降比值）则均随着光强的增加呈现递减的趋势，均在1 000 lx处理下呈现最大值，分别为0.847、5.573、0.815、4.200。
- Abstract:
- In order to establish suitable light quality and light intensity for the growth of red raspberry tissue culture plantlets，using red raspberry ‘Heritage’ tissue culture plantlets as experimental material，red raspberry root chlorophyll content and chlorophyll fluorescence parameters of tissue culture plantlets of different light quality and light intensity were studied.The results showed that，chlorophyll a，chlorophyll b and the total chlorophyll content increased first and then decreased with increasing of R/B ratio except R/B treatment，chlorophyll a and the total chlorophyll content showed a maximum value in the treatment of 2R/B，respectively 1.344 mg?g-1，1.716 mg?g-1，chlorophyll b was the highest in R/2B，was 0.423 mg?g-1，chlorophyll a，chlorophyll b and the total chlorophyll content(Ct) were the lowest in 10R/B，respectively 0.130 mg?g-1，0.033 mg?g-1，0.163 mg?g-1，were significantly lower than other light；chlorophyll a/b were higher in 10R/B，2R/B and R/B，were 3.942，3.709，3.653，showing the characteristics of sun plants，and in 6R/B，W and 4R/B were lower，were 2.729，2.730，2.747,and showing the characteristics of shade plants；under R/2B，the chlorophyll fluorescence parameters of raspberry plantlets leaves showed the excellent value，the Fv/Fm value reached 0.881，however 10R/B，Fv/Fm decreased to 0.355，and significantly lower than the other light treatment.In light intensity treatment，chlorophyll a，chlorophyll b and the total chlorophyll content showed a decreasing trend with the increase of light intensity，respectively dropped to 0.907 mg?g-1，0.302 mg?g-1，1.209 mg?g-1 from the maximum value of 1.218 mg?g-1，0.503 mg?g-1，1.720 mg?g-1，the formation of significant differences，chlorophyll a/b was increased with the increase of light intensity，in the 2 000 lx reached the maximum value，was 3.042，and three kinds of light intensity were significantly different；the initial fluorescence Fo of leaves increased with the increase of light intensity，in the light intensity of 2 000 lx to a maximum value，was 180.000，maximum fluorescence values appeared in 1 500 lx，was 907.000，however,PSII of maximal chemical efficiency Fv/Fm，PSII potential activity of Fv/Fo，PSII actual photochemical efficiency of PSII，variable fluorescence decreased ratio Rfd were with the light intensity increasing，which presented a decreasing trend，in 1 000 lx were presented maximum，were 0.847，5.573，0.815，4.200,respectively.
参考文献/References:
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备注/Memo
第一作者简介：郭芳（1990-），女，河北卢龙人，硕士研究生，研究方向为经济林栽培生理。E-mail:guofang901003@163.com.责任作者：李保国(1958-)，男，河北武邑人，博士，教授，博士生导师，现主要从事经济林栽培生理和山区开发技术研究及经济林栽培教学等工作。E-mail：lbg888@163.com.基金项目：河北省“十二五”科技支撑资助项目(16226806D)；国家现代农业科技成果惠民科技示范工程资助项目(Z141100002314009)。