GAO Yuan,LI Peng,ZHANG Qingchen,et al.Separation and Determination of Quinolizidine Aalkaloids in Callus of Sophora alopecuroides L.by HPLC and Its System Optimization[J].Northern Horticulture,2016,40(10):122-126.[doi:10.11937/bfyy.201610031]
HPLC法分离测定苦豆子愈伤组织喹诺里西啶生物碱含量及体系优化
- Title:
- Separation and Determination of Quinolizidine Aalkaloids in Callus of Sophora alopecuroides L.by HPLC and Its System Optimization
- Keywords:
- callus of Sophora alopecuroides L.; HPLC; oxysophocarpine; oxymatrine; sophoridine; sophocarpine; matrine
- 文献标志码:
- A
- 摘要:
- 以苦豆子愈伤组织为试材，采用高效液相色谱法，分离并测定苦豆子愈伤组织中氧化槐果碱、氧化苦参碱、槐定碱、槐果碱和苦参碱，建立了这5种单碱分离、测定的色谱方法。结果表明：色谱条件为UltimateAQ-C18(4.6 μm×250 mm×5 mm)；流动相为0.01 mol?L-1 磷酸缓冲液(K2HPO4=5.59 g?L-1，KH2PO4=0.41 g?L-1，pH 7.5)-甲醇(50∶50，V/V)；检测波长为216 nm；柱温为35 ℃；流速为1.0 mL?min-1，检测苦豆子愈伤组织中含有5种生物单碱，其中氧化槐果碱和槐定碱在苦豆子愈伤组织继代培养第4代时含量相对较高，分别为1.205 6 mg?g-1和0.116 5 mg?g-1，氧化苦参碱、槐果碱、苦参碱的含量略有波动，大致呈下降趋势。
- Abstract:
- Taking the callus of Sophora alopecuroides L.as the experimental materials，using the method of HPLC for the separation and determination of oxysophocarpine，oxymatrine，sophoridine，sophocarpine and matrine in callus of Sophora alopecuroides L.,a chromatographic method for the separation and determination of five kinds of alkaloids from Sophora alopecuroides L.was established.The results showed that，chromatographic conditions,Ultimate AQ-C18(4.6 μm×250 mm×5 mm)；mobile phase,0.01 mol?L-1 phosphate buffer(K2HPO4=5.59 g?L-1，KH2PO4=0.41 g?L-1，pH 7.5)-methanol(50∶50,V/V)；UV detection at 216 nm；column temperature,35 ℃;flow rate,1.0 mL?min-1.There were five kinds of alkaloids in the callus of Sophora alopecuroides L..The ingredients and contents of oxysophocarpine and oxymatrine were higher in subculture callus which were cultivated into stage 4.They were 1.205 6 mg?g-1 and 0.116 5 mg?g-1,sophoridine，sophocarpine and sophocarpidine were fluctuated slightly and downtrended more or less as the time went by.
参考文献/References:
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备注/Memo
第一作者简介：高媛（1988-），女，山东日照人，硕士研究生，研究方向为植物保护。E-mail：elfish521@163.com. 责任作者：顾沛雯（1969-），女，宁夏银川人，博士，教授，硕士生导师，现主要从事植物保护等研究工作。E-mail：gupeiwen2013@126.com. 基金项目：国家自然科学基金资助项目 (31260452)。