GUO Yinshan,NIU Zaozhu,SHI Guangli,et al.Genetic Diversity Analysis of Grape Resources Based on SSR Markers[J].Northern Horticulture,2016,40(07):89-92.[doi:10.11937/bfyy.201607023]
基于SSR分子标记的葡萄品种遗传多样性分析
- Title:
- Genetic Diversity Analysis of Grape Resources Based on SSR Markers
- Keywords:
- grape; SSR; PCR; clustering analysis; genetic relationship
- 文献标志码:
- A
- 摘要:
- 以15份葡萄品种为试材，采用SSR分子标记技术，研究了15份葡萄品种资源的遗传多样性。结果表明：从245对引物中筛选出49对清晰、稳定性好的多态性引物。49对引物在15份材料中共扩增出235条带，均为多态性条带，多态性百分率为100%。每对引物扩增的等位位点数为2~9个，相似性系数分析结果表明，供试的15份葡萄材料的遗传相似系数为0.660～0.860，其平均遗传相似系数为0.760。聚类分析表明，在遗传相似系数0.672处，15份葡萄材料可分为三大类群。第一大类包括2份欧美杂种（V.vinifera L.×V.labrusca L.）和1份美洲种（V.labrusca L.）品种；第二大类为8份欧亚种（V.vinifera L.）葡萄品种；第三大类包括2份山欧杂种（V.amurensis Rupr.×V.vinifera L.）和2份山葡萄（V.amurensis Rupr.）品种。可见，欧亚种和美洲种葡萄亲缘关系相对较近，而山葡萄与二者亲缘关系相对较远。这些引物中，VMCNG4B9、VMC3G9能将15个葡萄品种区分开，为今后开展葡萄品种指纹图谱构建奠定基础。
- Abstract:
- Taking 15 grape resources as test materials,using SSR molecular marker method，the genetic diversity for 15 grape resources was researched.The results showed that among 245 SSR markers，49 distinct and stability markers were selected to conduct the amplification for the 15 grape resources，in total of 235 polymorphic bands were got，the polymorphic percentage were 100%.The number of allelic loci for each primer were ranged from 2 to 9，based on the SSR amplification results，used the software of NTSYSpc2.10e to conduct the Jaccard correlation coeffiecient analysis for the 15 grape resources，the result was between 0.660-0.860，the average genetic correlation coeffiecient was 0.760.After UPGMA clustering analysis，the 15 grape resources could be divided into 3 groups.The first group contained 2 resources crossed by V.vinifera L.and V.labrusca L.and 1 resource from V.labrusca L.；the second group contained 8 V.vinifera L.resources；the third group contained 2 resources crossed by V.amurensis Rupr.and V.vinifera L.and 2 V.amurensis Rupr.resources.It showed that the V.vinifera L.and V.labrusca L.had the nearest genetic relationship，the genetic relationship of V.amurensis Rupr.was farther to V.vinifera L.and V.labrusca L..The primers of VMCNG4B9,VMC3G9 could separate these 15 grape resources，they could lay the foundation for the grape finger-print in the future.
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备注/Memo
第一作者简介：郭印山（1977-），男，博士，副教授，硕士生导师，研究方向为果树遗传育种与生物技术。E-mail：guoyinshan77@126.com. 责任作者：郭修武（1959-),男，博士，教授，博士生导师，研究方向为果树遗传育种与生物技术。E-mail:guoxw1959@163.com. 基金项目：国家自然科学基金资助项目（31000894,31372021）；国家现代农业产业技术体系建设专项基金资助项目（CRAS-30-yz-6）；辽宁省葡萄科技创新团队资助项目（2014204004）；高等学校博士点基金资助项目（20102103120003）；辽宁省教育厅资助项目（L2010492）；沈阳市人才资源开发专项资助项目（201107）。