JIN Feng,WANG Hui,WU Ya,et al.Occurrence and Pathogen Identification of Continuous Cultivation Eggplant Root-knot Nematode Disease in Greenhouse[J].Northern Horticulture,2016,40(03):117-120.[doi:10.11937/bfyy.201603033]
金凤,王慧,吴亚,陈启迪,顾百冲
- Title:
- Occurrence and Pathogen Identification of Continuous Cultivation Eggplant Root-knot Nematode Disease in Greenhouse
- Keywords:
- eggplant; root-knot nematode; pathogen identification; perineal-striae
- 文献标志码:
- A
- 摘要:
- 以大棚连茬茄子为调查对象,采用形态观察法,研究了感染茄子根结线虫的种类及危害。结果表明:3栋大棚茄子根结线虫病发病率达80%,根结指数为17.9;解剖镜下解剖观察根结,发现根结内有大量的雌虫和卵;分离获得雌虫、雄虫和2龄幼虫后分别进行观察和测量,并制作雌虫的会阴花纹切片进行比较鉴定,发现危害茄子的病原线虫为南方根结线虫(Meloidogyne incognita)。
- Abstract:
- Taking continuous cultivation eggplants as investigation objects,using morphology observation method,the species and harm of continuous cultivation eggplant’s root-knot nematode in greenhouse were investigated.The results showed that the incidence of eggplant root-knot nematode disease in 3 greenhouses was 80%,the root knot index was 17.9.There were a large number of females and eggs in the root knot under the microscope.Female,male and 2nd instar larva were isolated,observed and measured.Also perineal-striae was made in order to identify nematode species.The pathogen of eggplant root-knot nematode disease was Meloidogyne incognita.
参考文献/References:
[1]武杨,郑经武,商晗武,等.根结线虫分类和鉴定途径及进展[J].浙江农业学报,2005,17(2):106-110. [2]廖金铃,彭德良,段玉玺.中国线虫学研究:第4卷[M].北京:中国农业科学技术出版社,2012:99. [3]刘维志.植物线虫学研究技术[M].沈阳:辽宁科学技术出版社,1995:200-202. [4]泰勒,萨塞.植物根结线虫生物学、分类鉴定和防治[M].曾大鹏,译.北京:科学出版社,1983:150-154. [5]SCHMITZ B,BURGERMEISTER W,BRAASCH H.Molecular genetic classification of central European Meloidogyne chuwoodi and M.fallax populations[J].Nachrichtenblatt des Deutschen Pflanzenschutzdienstes,1999,51(12):310-317. [6]艾森拜克,赫什曼,萨塞.四种最常见根结线虫分类指南[M].杨宝君,译.昆明:云南人民出版社,1986:45-49. [7]高原,邓华,李春亮.线虫的分子生物学研究[J].生物学杂志,2003,20(5):4-5. [8]魏学军,杨文香,刘大群,等.植物根结线虫分子鉴定研究进展[J].中国农学通报,2006,22(8):401-404. [9]张靠稳,贾振华.根结线虫会阴花纹的染色方法[J].北方园艺,2008(3):207-208. [10]洪权春,胡海燕.商丘地区保护地番茄根结线虫种类鉴定[J].安徽农业科学,2008,36(25):10973-10974. [11]CHITWOOD B G.Root-knot nematodes-partⅠ.A revision of the genus Meloidogyne goeldi,1887[J].Proc Helminth Soc Wash,1949,16:80-104. [12]宋志强.江苏省蔬菜根结线虫的分布调查、种类鉴定及定量检测技术研究[D].南京:南京农业大学,2013. [13]梁建根,郑经武.设施栽培中蔬菜根结线虫生物防治研究进展[J].中国农学通报,2010,26(19):290-293.
相似文献/References:
[1]王晓娥,李建国,赵姝荣,等.咸阳市设施番茄根结线虫病的发生与防治[J].北方园艺,2013,37(04):133.
[2]赵静,夏海波,李艳青,等.根际促生菌YHN对番茄和茄子的促生作用研究[J].北方园艺,2014,38(04):30.
ZHAO Jing,XIA Hai-bo,LI Yan-qing,et al.Study on Growth-promoting Effect of Plant Growth-promoting Rhizobacteria Strain YHN on?the Seedling of Tomato and Eggplant[J].Northern Horticulture,2014,38(03):30.
[3]王铁良,陈思,毛丽珍.渗灌条件下土壤水分对温室秋冬茬茄子生长效应[J].北方园艺,2014,38(04):45.
WANG Tie-liang,CHEN Si,MAO Li-zhen.Effect of Soil Moisture on Growth of Greenhouse Eggplant With[J].Northern Horticulture,2014,38(03):45.
[4]樊绍翥,谷晓华,安凤霞.茄子耐热性状正态性遗传测验分析[J].北方园艺,2013,37(17):37.
FAN Shao-zhu,GU Xiao-hua,AN Feng-xia.Normality Hereditary Testing Analysis of Thermal Tolerance Traits with Eggplant[J].Northern Horticulture,2013,37(03):37.
[5]韩润燕,陈彦云,高新华.黄瓜根结线虫病防治药剂筛选试验[J].北方园艺,2014,38(06):120.
HAN Run-yan,CHEN Yan-yun,GAO Xin-hua.Screening of Nematocides for Control of Root-knot Nematode in Cucumber[J].Northern Horticulture,2014,38(03):120.
[6]狄文伟,周 彤,赵 冰.不同引发剂对茄子种子引发效果的研究[J].北方园艺,2014,38(09):12.
DI Wen-wei,ZHOU Tong,ZHAO Bing.Effects of Different Evocating Agent on the Evoked Response of Eggplant Seeds[J].Northern Horticulture,2014,38(03):12.
[7]叶景学,齐义杰,赵超男,等.等渗钠盐胁迫对茄子种子发芽特性的影响[J].北方园艺,2013,37(03):40.
YE Jing-xue,QI Yi-jie,ZHAO Chao-nan,et al.Effect of the Iso-osmotic Sodium Salt Stress on Germinating Characteristic of the Eggplant Seeds[J].Northern Horticulture,2013,37(03):40.
[8]张玉芹.枯草芽孢杆菌Bs-18菌株分离与鉴定及对根结线虫的防治效果[J].北方园艺,2012,36(01):130.
ZHANG Yu-qin.Screening and Identification of Bacillus subtilus Strain Bs-18 and Its Biocontrol Evaluation Against Meloidogyne incognita[J].Northern Horticulture,2012,36(03):130.
[9]卢树昌,刘慧芹,王小波,等.不同药剂对感染根结线虫黄瓜生理性状的影响[J].北方园艺,2012,36(01):132.
LU Shu-chang,LIU Hui-qin,WANG Xiao-bo,et al.Physiological Effect of Different Pesticides to Cucumber Infected by Meloidogyne spp.[J].Northern Horticulture,2012,36(03):132.
[10]李烨,谢立波,姚建刚,等.沼渣与基质配比对茄子幼苗的影响[J].北方园艺,2012,36(03):28.
LI Ye,XIE Li-bo,YAO Jian-gang,et al.Effects of Different Substrate with Biogas Residue Proportion on Seedlings of Eggplant[J].Northern Horticulture,2012,36(03):28.
[11]王冰林,韩太利,李媛媛.根结线虫侵染对茄子苗期生理生化反应的影响[J].北方园艺,2012,36(23):139.
WANG Bing-lin,HAN Tai-li,LI Yuan-yuan.Effects of Meloidogyne incognita Infection on Physiology and Biochemistry of Eggplant Seedling Leaves[J].Northern Horticulture,2012,36(03):139.
备注/Memo
第一作者简介:金凤(1973-),女,吉林抚松人,硕士,副教授,现主要从事昆虫生理及植物病虫害防治等研究工作。E-mail:jf888@126.com.基金项目:金陵科技学院博士科研启动基金资助项目。