HU Bingfen,ZHANG Baolin,CAI Guojun,et al.Genetic Diversity by SSR Markers in Seventeen Chinese and Amarican Lycium barbarum L.[J].Northern Horticulture,2016,40(01):90-94.[doi:10.11937/bfyy.201601024]
十七份中美枸杞材料的SSR遗传多样性
- Title:
- Genetic Diversity by SSR Markers in Seventeen Chinese and Amarican Lycium barbarum L.
- Keywords:
- Lycium; SSR markers; genetic diversity
- 文献标志码:
- A
- 摘要:
- 以17份中美枸杞材料为试材，采用SSR分子标记技术对其进行遗传多样性分析，探索中美枸杞的遗传多样性。结果表明：筛选的20对SSR引物扩增得到46个等位变异，平均每对引物扩增得到2.3个等位点，多态性比率达75.44%，多态性信息含量变化范围在0.114 2(SSR111)～0.829 1(SSR22)，平均0.377 2，说明中美枸杞的遗传多样性较低；聚类分析结果显示，在相似系数0.600处，所有供试材料被聚为一类；在相似系数0.730处，美国枸杞和中国枸杞分别独立聚类；在相似系数0.825处，兰山野生枸杞和国内其它枸杞分为2个类群；来自内蒙古和宁夏的枸杞也基本分组，但相似系数很高，说明所有供试材料遗传基础非常狭窄，聚类分析结果与供试材料区域来源有较好的一致性，同一栽培区域育成的品种在不同程度上聚为一类，不同品种间的亲缘关系与地区来源基本相对应。
- Abstract:
- To explore the genetic diversity of Chinese and Amarican Lycium，the genetic diversity of 17 Lycium materials were analyzed using SSR molecular marker technique in this study.The results showed that，46 alleles were amplified by 20 pairs of SSR primers and 2.3 alleles were amplified by per pairs，the ratio of polymorphism was 75.44%，the polymorphism information content ranged from 0.114 2(SSR111) to 0.829 1(SSR22)，with an average of 0.377 2.The diversity of the 17 Lycium materials showed low by these parameters.All the tested materials were clustered into one group based on the UPGMA cluster at the genetice similarity(GS) 0.600；American Lycium and Chinese Lycium were clustered respectively at 0.730；Lanshan wild gouqi and other domestic Lycium were divided respectively into two groups at 0.825；Lycium from Inner Mongolia and Ningxia were grouped roughly，but the GS was very high.It was indicated that the genetic basis of all the tested materials were narrow，and the clustering analysis results of them were consistent with the regional sources of the materials，and the varieties in the same cultivation area were clustered into one category，and the relationship between the cultivars and the region was basically the same.
参考文献/References:
[1]马德滋,刘慧兰.宁夏植物志[M].2卷.银川:宁夏人民出版社,1990:155-156. [2]钟鲑元.宁夏枸杞高产栽培与育种[M].银川:宁夏人民出版社,1995. [3]聂国朝.3种枸杞的HPLC-DAD图谱比较[J].福建林学院学报,2004,24(2):162-164. [4]马永平,赵海燕,杨少娟.枸杞多种同工酶水平的遗传多样性分析[J].安徽农业科学,2010,38(8):4042-4043. [5]魏玉清,许兴.不同地区主要栽培宁夏枸杞品种的RAPD分析[J].西北农林科技大学学报,2007,35(1):91-95. [6]尚洁,思彬彬.宁夏枸杞主要栽培品种的DNA多态性分析[J].安徽农业科学,2010,38(6):2801-2802. [7]张波,李敦,戴国礼.22份枸杞属种质的RAPD分析[J].广东农业科学,2012(5):112-113,127. [8]石志刚,安巍,樊云芳,等.枸杞nrDNA ITS测序鉴定的初步研究[J].安徽农业科学,2008,36(16):6687-6688,6690. [9]石志刚,安巍,焦恩宁,等.基于nrDNA ITS测序的18份宁夏枸杞资源的遗传多样性[J].安徽农业科学,2008,36(24):10379-10380. [10]李彦龙,樊云芳,戴国礼.枸杞种质遗传多样性的AFLP分析[J].中草药,2011,42(4):770-773. [11]鲍红春,李小雷,王建平.枸杞遗传多样性的ISSR分析[J].华北农学报,2014,29(1):89-92. [12]段丽君,曹有龙,周军.枸杞ISSR反应体系的优化与应用[J].西北农林科技大学学报,2009,37(12):133-138. [13]赵军海,冯国华,刘东涛,等.小麦育种亲本材料遗传多样性的SSR分析[J].麦类作物学报,2009(6):982-985. [14]高翔,庞红喜,裴阿卫.分子标记技术在植物遗传多样性研究中的应用[J].河南农业大学学报,2002,36(4):356-359. [15]刘列钊,林呐.油菜简单重复序列SSR(Simple Sequence Repeat)研究进展[J].生命科学,2004,16(3):173-176. [16]GHISLAIN M,SPOONER D M,RODRGUEZ F,et al.Selection of highly informative and user-friendly microsatellites (SSRs) for genotyping of cultivated potato[J].Theor Appl Genet,2004,108:881-890. [17]滕长才,张永成,张凤军,等.青海省马铃薯主要栽培品种的SSR遗传多样[J].分子植物育种,2009,7(3):555-561. [18]NUNOME T,NEGORO S,KONO I,et al.Development of SSR markers derived from SSR-enriched genomic library of eggplant(Solanum melongena L.)[J].Theoretical and Applied Genetics,2009,19(6):1143-1153. [19]BINDLER G,van DER HOEVEN R,GUNDUZ I,et al.A microsatellite marker based linkage map of tobacco[J].Theor Appl Genet,2007,114:341-349. [20]罗玉娣,李建国,李明芳.用SSR标记分析辣椒属种质资源的遗传多样性[J].生物技术通报,2006(增刊):338-340. [21]WANG A X,MENG F J,XU X Y,et al.Development of molecular markers linked to Cladosporium fulvum resistant gene Cf-6 in tomato by RAPD and SSR methods[J].Horticulture Science,2007,42(1):11-15. [22]王柏柯,余庆辉,杨生保.4个加工番茄新品种SSR指纹图谱的构建与品种鉴定[J].新疆农业科学,2010,47(7):1474-1478. [23]许向阳,孟凡娟,李景富.番茄资源对叶霉病的抗性鉴定和SSR标记遗传变异分析[J].植物病理学报,2007,37(5):520-527. [24]SULIMAN P S,KASHKUSH K,SHAT S H,et al.Generation and mapping of AFLP，SSRs and SNPs in Lycopersicon esculentum[J].Cell Mol Biol Lett,2002,7(2A):593-597.
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备注/Memo
第一作者简介：胡秉芬（1969-），女，甘肃景泰人，博士，副研究员，现主要从事经济林育种等研究工作。E-mail：bf9134hu@163.com.责任作者：张宝琳（1968-），女，硕士，高级工程师，现主要从事经济林等研究工作。E-mail：530139563@qq.com.基金项目：国家林业局“948”资助项目（2011-4-39）。