LIU Wei,DING Changchun,CHANG Zheng,et al.Study on Pollen Storage Condition of Lilium sulphureum Baker[J].Northern Horticulture,2015,39(23):108-111.[doi:10.11937/bfyy.201523030]
淡黄花百合花粉离体保存条件研究
- Title:
- Study on Pollen Storage Condition of Lilium sulphureum Baker
- Keywords:
- Lilium sulphureum Baker; pollen viability; storage condition
- 文献标志码:
- A
- 摘要:
- 为了获得最适合淡黄花百合花粉离体保存的条件，以不同发育阶段的淡黄花百合花粉为试材，采用TTC法测定花粉萌发率，研究了4种贮藏温度对3个发育阶段淡黄花百合花粉生命力的影响。结果表明：取样当天测定开花第3天的花粉生命力最强，萌发率为 91.97%；开花当天的花粉萌发率为73.63%；开放前2 d的花粉萌发率为70.60%。在不同温度下离体保存后，随着贮藏时间的延长，所有花粉的萌发率在前4 d迅速下降，4 d后下降变缓。通过对比，开花前2 d和开花当天的花粉贮藏在4℃的条件下其生命力保持较好，保存时间不超过8 d为宜。
- Abstract:
- Pollen of Lilium sulphureum Baker was employed as experimental material to carry on studies on viability and storage condition.Pollen viability of 3 development periods and 4 temperature conditions were studied，and the germination rate was tested by TTC in these experiments.The results showed that fresh pollen of different development period had different viability.The pollen germination rate after flowering three days was 91.97%，flowering day was 73.63%，and two days before flowering was 70.60%.Pollen from undeveloped bud and new blooming bud had the longest shelf life after storage.Storage temperature had great effect on pollen viability.4℃ was favorable for keeping pollen viability，and -20℃ took the second place，but the storage period should be within 8 days.
参考文献/References:
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备注/Memo
第一作者简介：刘伟(1977-)，男，硕士，副教授，现主要从事植物学等研究工作。E-mail:liuwei00780@126.com.基金项目：云南省教育厅自然科学研究资助项目（2013Y582）；文山学院重点学科资助项目（09wsxk 02）。