LI Li,LI Xiaojiao,XIONG Yanhua,et al.Study on Steam Distillation Combined With Microwave Extraction and Anti-microbial Activities of Essential Oils From the Leaves of Elsholtzia rugulosa[J].Northern Horticulture,2015,39(22):137-140.[doi:10.11937/bfyy.201522036]
微波辅助水蒸气蒸馏法提取野坝子叶精油及其抑菌活性测试
- Title:
- Study on Steam Distillation Combined With Microwave Extraction and Anti-microbial Activities of Essential Oils From the Leaves of Elsholtzia rugulosa
- 文献标志码:
- A
- 摘要:
- 以野坝子叶为原料，蒸馏水为提取剂，采用单因素试验和正交实验相结合，研究了料液比、NaCl质量分数、微波功率、微波时间等工艺参数对精油得率的影响，并采用滤纸片法和2倍稀释法测野坝子叶精油对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的体外抗菌活性。结果表明：野坝子叶精油的最佳提取工艺为料液比1∶20 g/mL，NaCl的质量分数1%，微波功率350 W，微波时间30 s，精油平均得率为2.01%。对3种细菌的体外抗菌试验表明，该精油对测试菌株具有较好的抑菌能力。
- Abstract:
- Taking the leaves of Elsholtzia rugulosa as test materials,and distilled water as extractant,the technology of extraction of the essential oils from the leaves of Elsholtzia rugulosa was studied to provide a reference for further study and utilization，the effect of solid-1iquid ratio，NaCl concentration，extracting time and microwave power on the extracttion of the essential oils from the leaves of Elsholtzia rugulosai was investigated using the methods of single factor test and orthogonal design test of L9(34).Meanwhile，the bacteriostatic test of resulting extract was further emulated to Escherichia coli，Bacillus subtilis and Staphylococcus aureus by filter paper method and two times dilution method.The results showed that highest extraction rate (2.01%) of the essential oils in the leaves of Elsholtzia rugulosa could be obtained under conditions of 1∶20 g/mL，the NaCl was 1% (wt%)，microwave power was 350 W，and extracting time was 30 seconds.In addition，the essential oil had certain antibacterial activity to the tested microbial.
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备注/Memo
第一作者简介：李丽（1982-），女，云南昌宁人，硕士，讲师，研究方向为催化化学。E-mail：lili19820607@126.com.基金项目：保山学院科研教研基金重点资助项目（13BZ012）。