LIU Zhao.Study on Propagation in vitro of Chongming Narcissus Bulbs (Narcissus tazetta var.chinensis)[J].Northern Horticulture,2015,39(22):99-102.[doi:10.11937/bfyy.201522026]
崇明水仙鳞茎的组织培养研究
- Title:
- Study on Propagation in vitro of Chongming Narcissus Bulbs (Narcissus tazetta var.chinensis)
- 文献标志码:
- A
- 摘要:
- 以崇明水仙鳞茎为外植体进行试管种球的诱导,对其组织培养中消毒条件、植物激素、生根移栽技术等影响因子进行研究。结果表明:诱导培养的最佳培养基为MS+6-BA 5 mg/L+NAA 1 mg/L,诱导率为100%;增殖培养的适宜培养基是MS+6-BA 1 mg/L+NAA 1 mg/L+2,4-D 0.1 mg/L+IBA 0.5 mg/L,增殖系数达3.43;小鳞茎经壮苗培养20 d后移栽到珍珠岩基质中即可生根成活,移栽成活率达90.5%。
- Abstract:
- This paper was mainly discussed about the propgation in vitro of Chongming narcissus (Narcissus tazetta var.chinensis).The results showed that segments of the bulbs with basal scales and a portion of the plate,MS medium containing 5 mg/L+6-BA+1 mg/L NAA was suitable for induction of bulblets of Chongming narcissus,and the frequency of induction was 100%.The MS medium,supplemented with 1 mg/L 6-BA,1 mg/L NAA,0.1 mg/L 2,4-D and IBA 0.5 mg/L could be used for multiplication induction,and the proliferation was 3.43.After 20 days for culturing strong plants,tranfered the plantlets into perlite.The plantlets started rooting about two weeks.And the survival rate of 90.5% in two months.
参考文献/References:
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备注/Memo
作者简介:刘炤(1983-),男,硕士,工程师,现主要从事花卉种质资源收集与遗传育种等研究工作。E-mail:liuzhaojob@126.com.基金项目:上海市科学技术委员会科研资助项目(13231204300)。