WANG Jianshu,SUN Songsong,LIU Hao,et al.Induction of Direct Shoot Organogenesis From Shoot Tip Explants of Cucurbita pepo L.[J].Northern Horticulture,2015,39(22):96-98.[doi:10.11937/bfyy.201522025]
西葫芦茎尖离体培养和快速繁殖技术
- Title:
- Induction of Direct Shoot Organogenesis From Shoot Tip Explants of Cucurbita pepo L.
- 关键词:
- 西葫芦（Cucurbita pepo L.）; 茎尖; 组织培养; 快繁
- Keywords:
- Cucurbita pepo L.; shoot tip; tissue culture; rapid propagation
- 文献标志码:
- A
- 摘要:
- 以西葫芦茎尖为外植体，MS为基本培养基，探索了不同消毒处理、不同浓度的植物生长调节剂及其组合对西葫芦茎尖再生和快速繁殖的影响。结果表明：西葫芦茎尖在含有0.02%升汞与10%次氯酸钠的溶液中消毒10 min的效果最好，污染率降低到6.7%，成活率可达91.7%；西葫芦茎尖诱导再生芽的最好培养基处理为MS+6-BA 0.5 mg/L+IBA 0.10 mg/L；最佳的生根诱导培养基为MS+IBA 0.30 mg/L。
- Abstract:
- The study described an efficient in vitro micropropagation protocol for induction of multiple shoots from shoot tip explants of Cucurbita pepo L.The results showed that shoot tip explants were treated with 10% solution of sodium hypochlorite，which contain 0.02% mercury chloride (HgCl2)，for 10 minutes，the lowest contamination rate was 6.7%，the highest survive rate was 91.7%.The highest frequency (100%) of multiple shoot formation with maximum number of shoots (2.38 shoots per explants) was achieved on MS medium supplemented with 0.5 mg/L 6-BA and 0.10 mg/L IBA.Shoots were rooted most effectively in 0.30 mg/L indole-3 butyric acid (IBA)-supplenmented MS medium，and all rooted shoots were successfully transplanted into soil.
参考文献/References:
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备注/Memo
第一作者简介：王建书（1960-），男，博士，教授，研究方向为植物学。E-mail：wangjianshu135@163.com.基金项目：农业部公益性行业专项资助项目（201303112）；“十一五”、“十二五”科技支撑资助项目（2011BAD35B07,2012BAD02B03,2012BAD50G01）；科技部“863”资助项目（2012AA100202-3）。