ZHAO Ying,HUANG Guodi,FU Haitian,et al.SRAP Analysis of 12 Mango Germplasm Genetic Relationships[J].Northern Horticulture,2015,39(21):99-102.[doi:10.11937/bfyy.201521026]
十二份杧果种质亲缘关系的SRAP分析
- Title:
- SRAP Analysis of 12 Mango Germplasm Genetic Relationships
- Keywords:
- mango; SRAP; genetic relationship
- 文献标志码:
- A
- 摘要:
- 以12份杧果种质为试材,利用SRAP分子标记技术对其进行遗传多样性研究。结果表明:从36对引物中筛选出22对多态性好的引物,共扩增出251条DNA条带,其中多态性谱带181条,平均每对引物扩增得到15.08条多态性谱带,多态性比率为72.11%。UPGMA聚类表明,所有供试品种(系)之间的亲缘关系都比较近,相似系数在0.77~0.89;如果以相似系数0.78为标准,可将供试的12个品种(系)分为3类。表明利用SRAP技术能较好的区分杧果的亲缘关系;SRAP分子标记适合杧果的DNA遗传多样性分析,可作为杧果种质鉴定依据之一。
- Abstract:
- Taking 12 mango germplasms as metarials,SRAP marker was used to analyze genetic diversities.The results showed that 22 primers selected from 36 SRAP-primer were used for amplification and 251 DNA bands were obtained,including 181 polymorphic bands.Each primer amplified 15.08 polymorphic bands.The percent of polymorphic was 72.11%.Based on UPGMA analysis,all samples showed higher similarities and teir coefficients from 0.77 to 0.89.If the coefficient 0.78 was regarded as divided line of their genetic relationship,12 materials could be clustered into 3 groups by SRAP.The study indicated that the genetic relationship of mango cultivars or lines could be better assessed by SRAP which could be used in mango germplasms identification.
参考文献/References:
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备注/Memo
第一作者简介:赵英(1980-),女,硕士,助理研究员,现主要从事杧果育种等研究工作。E-mail:zhaoying-222@163.com.责任作者:黄国弟(1965-),男,硕士,研究员,现主要从事杧果种质资源及育种与栽培等研究工作。E-mail:gxhgd@126.com.基金项目:广西壮族自治区亚热带作物研究所专项资金资助项目(桂热研201305);广西壮族自治区亚热带作物研究所专项资金资助项目(桂热研201504);广西壮族自治区自然科学基金资助项目(2015GXNSFBA139046)。