ZHAO Yan,CHE Weiguang,BI Yufen.Thermotolerance Comprehensive Evaluation of Medicago sativa L.‘Deqin’ on Seedling Stage[J].Northern Horticulture,2015,39(20):69-73.[doi:10.11937/bfyy.201520018]
“德钦”紫花苜蓿苗期耐热性综合评价
- Title:
- Thermotolerance Comprehensive Evaluation of Medicago sativa L.‘Deqin’ on Seedling Stage
- Keywords:
- Medicago sativa L.‘Deqin’; fall dormancy rate; seedling stage; thermotolerance; comprehensive evaluation
- 文献标志码:
- A
- 摘要:
- 以“阿尔冈金”（低秋眠级，2）、“赛迪10” （高秋眠级，9）和“德钦” （低秋眠级，1.2）为试材，采用了30℃/35℃（夜温/昼温）长期高温处理方法，研究了高温胁迫对“德钦”苗期的光化学效率、相对电导率、丙二醛含量、相对含水量、细胞质和细胞膜蛋白质的影响，以探明云南野生栽培品种“德钦”紫花苜蓿苗期的耐热性。结果表明：3个紫花苜蓿品种苗期的耐热性强弱为：“赛迪10”（1.00）>“德钦”（0.47）>“阿尔冈金”（0.06）。“赛迪10”为耐热性强的品种；“德钦”耐热性中等；“阿尔冈金”为耐热性弱的品种。苗期“德钦”的耐热性强于“阿尔冈金”，弱于“赛迪10”。“德钦”的耐热性可能与其高温胁迫下维持细胞膜的完整性和叶片持水能力强有关，也与其机体对膜蛋白质的保护有关。
- Abstract:
- To investigate the thermotolerance of ‘Deqin’ (low fall dormancy rate，1.2) on seedling stage，this experiment was carried out with three alfalfa cultivars,‘Algonquin’ (low fall dormancy rate，2)，‘Sardi 10’ (high fall dormancy rate，9) and ‘Deqin’，by comparing the changes of Fv/Fm，EL，MDA，RWC，cytoplasmic protein and membrane protein among their leaves under heat stress (35℃/30℃，day/night).The experiment results showed that the thermotolerance rank of these three cultivars was ‘Sardi 10’ (1.00)>‘Deqin’(0.47)>‘Algonquin’(0.06).‘Sardi 10’ was thermotolerant，‘Algonquin’ was thermosensitive and ‘Deqin’ was mediate.The specific heat tolerance reason of ‘Deqin’ might be related to its maintaining the membrane integrity，holding water in leaves and protecting membrane protein.
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备注/Memo
第一作者简介：赵雁（1974-），女，博士，副教授，研究方向为牧草种质资源与育种。E-mail：744234036@qq.com.责任作者：毕玉芬（1960-），女，博士，教授，研究方向为牧草种质资源与育种。E-mail：biyufenynnd@sina.com.基金项目：国家自然科学基金资助项目（31160480）。