ZHANG Hongyan,REN Yongyang,CAO Xinyu,et al.Study on Pathogen Identification and Biological Characterstics of Ginseng Rust Rot[J].Northern Horticulture,2015,39(19):118-122.[doi:10.11937/bfyy.201519029]
人参锈腐病病原菌鉴定及生物学特性研究
- Title:
- Study on Pathogen Identification and Biological Characterstics of Ginseng Rust Rot
- 文献标志码:
- A
- 摘要:
- 以人参锈腐病病株根部分离的菌株R2为试材，采用传统的形态学观察与现代分子生物学技术相结合的方法进行了病原菌鉴定，并研究其生物学特性。结果表明：该病原菌为粪壳菌纲（Sordariomycetes），肉座菌目（Hypocreales），叶赤壳科（Nectriaceae），柱孢属（Cylindrocarpon），毁灭柱孢菌（Cylindrocarpon destructans）。该菌在马铃薯蔗糖琼脂培养基（PDA）和察氏培养基（CZA）上生长较快，最适的碳、氮源为蔗糖和尿素，最适生长温度为20~25℃，最适pH值为中性偏酸环境。
- Abstract:
- Taking phytopathogenic fungus R2 as experimental material which was isolated from Tiande ginseng base in the south of Xiaoxinganling mountain，Tieli，Heilongjiang.Identification and the biological characteristics were conducted by the combination of morphological observation and modern molecular biological technique were studied.The results showed that the pathogen R2 was Cylindrocarpon destructans，colony grew well on PDA and CZA，the optimum carbon source and nitrogen source were sucrose and urea，the suitable pH value for mycelia growth was pH 6-7，and the optimum temperature was 20-25℃.
参考文献/References:
[1]张怡轩,陈晓莹,赵文倩．人参皂苷生物转化的研究进展[J]．沈阳药科大学学报,2008,25(5)：419-422. [2]李玉．农业植物病理学[M]．长春:吉林科学技术出版社,1992. [3]白容霖．我国人参锈腐病综合防治研究进展[J]．植物保护,1990,16(2):40-41. [4]马凤茹,邢云章,王韵秋,等.人参锈腐病调查方法初探[J].中药材科技,1983(5):9-11. [5]戚佩坤,白金铠．吉林省栽培植物真菌病害志[M].北京：科学出版社,1966. [6]赵曰丰．关于人参锈腐病菌属名的修正[C]//全国科研系统人参学术会议汇编,1982. [7]徐中志．人参锈腐病研究概况[J]．云南农业科技,1994（1）：19-21. [8]方中达．植病研究法[M]．3版.北京：中国农业出版社,1998. [9]程丽娟,薛泉宏．微生物学实验技术[M]．西安：世界图书出版公司,2000. [10]孙广宇,张雅梅,张荣．突脐孢属Brn1基因核苷酸序列比较及系统发育研究[J]．菌物学报,2004,23（4）：480-486. [11]王玲娜,薛泉宏,唐明,等．内蒙古芹菜根腐病病株和健株根域土壤的微生物生态研究[J]．西北农林科技大学学报（自然科学版）,2010,38（8）:167-172,181. [12]CUI X L，MAO P，ZENG M.Streptimonospora salina gen.nov.，sp.nov.， a new member of the family Nocardiopsaceae[J].International Journal of Systematic and Evolutionary Microbiology，2001,51：357-363. [13]魏景超．真菌鉴定手册[M]．上海：上海科学技术出版社,1979. [14]GERLACH W，NIELSSON L.Beitrge zur Kenntnis der Gattung Cylindrocarpon Wr.V.Nectria radicicola n.sp.，die bisher unbekannte Hauptfruchtform von Cylindrocarpon radicicola Wr[J].Phytopathol Z，1963,48:251-257. [15]HALLEEN F，SCHROERS H J，GROENEWALD J Z，et al.Novel species of Cylindrocarpon (Neonectria) and Campylocarpon gen.nov.associated with black foot disease of grapevines (Vitis spp.) [J].Stud Mycol，2004，50：431-455. [16]WHETZEL H H，ROSENBAUM J.The disease of ginseng and their control[M].Washington:Bull.No.250.U.S.dep.Agriculture-bureau of Plant Industry.，1912. [17]ZIEZOLD M，HALL R，REELEDER R D，et al.Toxicity of fungicides in vitro to Cylindrocarpon destructans[J].Journal of Ginseng Research，1998，22:223-228.
相似文献/References:
[1]马赛,刘洪章.人参的研究及展望[J].北方园艺,2013,37(15):205.
MA Sai,LIU Hong-zhang.Research and Prospects of Ginseng[J].Northern Horticulture,2013,37(19):205.
[2]王燕,王春伟,高洁,等.不同杀菌剂及其配比对人参菌核病菌的毒力测定[J].北方园艺,2014,38(07):115.
WANG Yan,WANG Chun-wei,GAO Jie,et al.Toxicity Test of Different Fungicides and Its Mixed Preparations Against Sclerotinia schinseng[J].Northern Horticulture,2014,38(19):115.
[3]栾树昆,杨广顺,孙春玉,等.吉林抚松人参遗传多样性的SSR分析[J].北方园艺,2012,36(15):133.
LUAN Shu-kun,YANG Guang-shun,SUN Chun-yu,et al.SSR Analysis on Genetic Diversity of Jilin Fusong Panax ginseng[J].Northern Horticulture,2012,36(19):133.
[4]张丹,边贞贞,杨广顺,等.人参悬浮细胞系的建立及生长特性研究[J].北方园艺,2012,36(06):99.
ZHANG Dan,BIAN Zhen-zhen,YANG Guang shun,et al.Studies on Establishment and Characterization of Suspension Cells in Panax ginseng[J].Northern Horticulture,2012,36(19):99.
[5]刘哲,陈晓林,张连学.不同土壤环境人参中蛋白质含量与氮含量的对比分析研究[J].北方园艺,2013,37(18):151.
LIUZhe,CHENXiao-lin,ZHANGLian-xue.ContrastandAnalysisontheContentofGinsengProteinand NitrogenContentinDifferentSoils[J].Northern Horticulture,2013,37(19):151.
[6]何宛晟,韩忠明,韩梅,等.不同年限人参根际土壤酶活性动态研究[J].北方园艺,2014,38(19):157.
HE Wan-sheng,HAN Zhong-ming,HAN Mei,et al.Dynamic Variation of Rhizosphere Soil Enzyme Activities from Panax ginseng Fields in Different Cultivated Years[J].Northern Horticulture,2014,38(19):157.
[7]张 影,李函颖.吉林省不同产地人参中微量元素含量测定[J].北方园艺,2015,39(03):135.[doi:10.11937/bfyy.201503039]
ZHANG Ying,LI Han-ying.The Contents Determination of Trace Elements in Panax ginseng from Different Regions in Jilin Province[J].Northern Horticulture,2015,39(19):135.[doi:10.11937/bfyy.201503039]
[8]许 永 华,金 慧,庞 立 杰,等.老参地土壤改良及人参栽培技术[J].北方园艺,2010,34(14):0.[doi:10.11937/bfyy.2010014072]
XU Yong -hua,JIN Hui,PANG Li -jie,et al.Improvement of Soil Harvested Ginseng and Cultivation Ginseng T echnique[J].Northern Horticulture,2010,34(19):0.[doi:10.11937/bfyy.2010014072]
[9]杨艳文,姜沅彤,张连学.农田参和伐林参根际土壤酶指标与化学成分比较[J].北方园艺,2016,40(17):157.[doi:10.11937/bfyy.201617038]
YANG Yanwen,JIANG Yuantong,ZHANG Lianxue.Study on the Relationship Between Soil Enzyme Activity and Chemical Composition in Farmland and Deforestation Ginseng[J].Northern Horticulture,2016,40(19):157.[doi:10.11937/bfyy.201617038]
[10]张影,王云华,薛晓丽.吉林省不同产地人参中人参皂苷含量测定[J].北方园艺,2016,40(01):137.[doi:10.11937/bfyy.201601036]
ZHANG Ying,WANG Yunhua,XUE Xiaoli.Contents Determination of Ginsenoside in Panax ginseng From Different Regions in Jilin Province[J].Northern Horticulture,2016,40(19):137.[doi:10.11937/bfyy.201601036]
备注/Memo
第一作者简介：张鸿雁（1970-），女，黑龙江宝清人，博士，教授，研究方向为微生物资源与利用。E-mail：zhy2219@163.com.基金项目：黑龙江八一农垦大学博士启动基金资助项目(XDB2015-17)。