XIU Jingrun,DAI Yue,PIAO Xuanchun,et al.Plantlet Culture in Bioreactor and the Changes of Leave Stomata During Transplanting of Grape[J].Northern Horticulture,2015,39(19):26-29.[doi:10.11937/bfyy.201519007]
葡萄试管苗的反应器培养及其移栽过程中气孔变化
- Title:
- Plantlet Culture in Bioreactor and the Changes of Leave Stomata During Transplanting of Grape
- Keywords:
- grape; bioreactor; transportation; stomata
- 文献标志码:
- A
- 摘要:
- 以葡萄组培茎段为外植体，利用气升式生物反应器接触法培养，研究了不同蔗糖浓度对葡萄苗生长的影响，同时在栽培后调查成活率并观察气孔行为，以寻找一种简单、低成本及规模化培养优良葡萄苗的模式。结果表明：当蔗糖浓度为20 g/L时苗的叶片颜色浓绿，节数多，苗和根的生长均良好。将反应器培养的葡萄苗分别移栽到蛭石和珍珠岩不同比率的移栽基质中，发现当蛭石量多时有利于葡萄苗的成活，单独使用蛭石的效果最佳，移栽30 d后成活率达到100%；对反应器培养葡萄苗移栽过程中气孔进行观察，移栽后10 d起部分气孔开始恢复关闭功能，14 d后大部分（90%以上）气孔可以关闭，所以在对反应器葡萄苗进行移栽时，0~10 d需进行保湿措施，14 d以后可进行常规管理。
- Abstract:
- In order to explore a simple and low cost method for large scale production of plantlets in grape，with grape tissue cultured stem as explant，used air-lift bioreactor with raft method to study effect of sucrose concentrations on plantlet growth.Meanwhile，survival rate and stoma were observed after transplanting.The results showed that 20 g/L sucrose promoted plantlet growth，the plantlets with more numbers of node，deep green leaves，and high shoot and root biomass were obtained.The plantlets harvested from bioreactors were transplanted into substrates containing different amount of vermiculite and perlite，vermiculite was favorable for plantlet survive，all plantlets survived in the substrate which contained sole vermiculite.The close function in several stomata began from 10 days after transplanted，and the most of stomata were closed after 14 days.Therefore，the plantlets from bioreactors required moisture from beginning to 10 days after transplanted，and regular management was needed after 14 days after transplanted.
参考文献/References:
[1]陈玉庆.葡萄酒的成分与营养价值[J].酿酒,2004,31(5):112-114. [2]郑平生,赵贵宾,康天兰,等.我国葡萄嫁接栽培研究进展[J].北方园艺,2009(7):146-148. [3]张玉星.果树栽培学各论[M].北京:中国农业出版社,2003. [4]张爱华,容新民,李玉国.组织培养脱毒技术在新疆葡萄快繁中的应用[J].农业科技通报,2008(4):49-50. [5]袁惠君,宋占午,丁兰,等.人参果试管苗与移栽成活苗气孔行为的比较[J].西北师范大学学报,2001,37(4):89-92. [6]袁惠君,宋占午,杨红,等.草莓试管苗与土培苗气孔形态和气孔密度的比较[J].兰州理工大学学报,2004,30(5):79-80. [7]徐志刚,崔瑾,焦学磊.光照强度和CO2浓度间接调控对甘薯无糖组培苗光合特性的影响[J].南京农业大学学报,2004,27(1):11-14. [8]赵惠祥,顾乃良,张文庆.珠美海棠试管苗移栽前后气孔及根系状况的观察[J].华北农学学报,1990,5(3):52-55. [9]李睿,赵姝丽,毛艇,等.水稻剑叶气孔性状QTL分析[J].中国水稻科学,2010,6(24):659-662. [10]LU P，ZHANG S Q，WILLIAM H.Sucrose：a solute that accumulates in the guard-cell apoplast and guard-cell symplast of open stomata[J].FEBS Letters,1995,3(62):180-184.
相似文献/References:
[1]单守明,平吉成,刘晶,等.6-BA对染卷叶病毒葡萄光合作用和果实品质的影响[J].北方园艺,2013,37(04):5.
[2]梁浩.枣庄地区气候因子对葡萄生产模式选择的影响[J].北方园艺,2013,37(04):55.
[3]张广和,于晓丽,栾炳辉,等.倍创与杀菌剂混用对葡萄果实病害的防效评价[J].北方园艺,2013,37(04):127.
[4]李海峰,王瑞华,韩琛,等.新疆葡萄平衡施肥技术发展现状及展望[J].北方园艺,2013,37(04):185.
[5]史洪琴,蒋丽光.中短剪葡萄结实特性比较[J].北方园艺,2014,38(12):34.
SHI Hong-qin,JIANG Li-guang.Comparison of Grape Fruit Characteristics After Moderate and Short Pruning[J].Northern Horticulture,2014,38(19):34.
[6]张剑侠,吴行昶,杨亚州.葡萄种间杂种的抗寒性评价[J].北方园艺,2014,38(13):1.
ZHANG Jian-xia,WU Xing-chang,YANG Ya-zhou.Cold-resistance Evaluation in Grape Interspecific Hybrids[J].Northern Horticulture,2014,38(19):1.
[7]商佳胤,田淑芬,集 贤,等.果袋颜色对设施葡萄果实品质的影响[J].北方园艺,2014,38(11):42.
SHANG Jia-yin,TIAN Shu-fen,JI Xian,et al.Effect of Fruit Bag Color on Grape Quality in Facility Condition[J].Northern Horticulture,2014,38(19):42.
[8]王文平.肃州区非耕地日光温室葡萄反季节高效节水栽培技术[J].北方园艺,2014,38(10):43.
[J].Northern Horticulture,2014,38(19):43.
[9]周 恒,饶 鹏,田如英,等.不同栽培方式下五个葡萄品种在贵州铜仁地区的生产表现[J].北方园艺,2014,38(14):22.
ZHOU Heng,RAO Peng,TIAN Ru-ying,et al.Five Grape Varieties Under Different Cultivations in the Production Performance in Guizhou Tongren Area[J].Northern Horticulture,2014,38(19):22.
[10]吴久赟,郭峰,杨承时,等.不同叶果比对葡萄果实生长的影响[J].北方园艺,2014,38(04):33.
WU Jiu-yun,GUO Feng,YANG Cheng-shi,et al.Study on the Influence of Different Leaf-fruit Ratio on Growth of Grape[J].Northern Horticulture,2014,38(19):33.
备注/Memo
第一作者简介：修景润（1981-），男，硕士，助理研究员，研究方向为长白山经济植物开发与利用。E-mail：jingrunxiu@163.com.责任作者：金美玉（1985-），女，硕士，讲师，现主要从事植物组织培养等研究工作。E-mail：jinmeiyu@ybu.edu.cn.