MA Qian,RAO Jianhua.Determination of Ammonium Alkaloids in Methanol Extract of Lycium barbarum L.by Filtrate and Precipitation Methods[J].Northern Horticulture,2015,39(18):142-145.[doi:10.11937/bfyy.201518038]
滤液法和沉淀法测定宁夏枸杞果实醇提物中季铵型生物碱的含量
- Title:
- Determination of Ammonium Alkaloids in Methanol Extract of Lycium barbarum L.by Filtrate and Precipitation Methods
- Keywords:
- :Lycium barbarum L.; ammonium alkaloids; Reinecke`s salt; spectrophotometry; filtrate method; precipitation method
- 文献标志码:
- A
- 摘要:
- 以宁夏枸杞为试材，基于季铵型生物碱与雷氏盐的反应原理，采用分光光度法测定宁夏枸杞果实醇提物中季铵型生物碱含量，建立了宁夏枸杞果实醇提物中季铵型生物碱的含量测定方法。结果表明：滤液法与沉淀法的平均回收率分别为97.57%与98.06%，RSD分别为1.02%与1.10%(n=6)。由此看出，滤液法与沉淀法用于季铵型生物碱的含量测定，所得结果基本一致，滤液法操作相对简便。
- Abstract:
- Lycium barbarum L. was used as raw material，and spectrophotometry was used to determine content of ammonium alkaloids in methanol extract of Lycium barbarum L.，based on the reaction of ammonium alkaloids with Remecke salt.The aim was to establish a method for quantitative determination of ammonium alkaloids in methanol extract of Lycium barbarum L.The results showed that the average recovery rate of filtrate and precipitation methods was 97.57%and 98.06%，and relative standard deviation was 1.02%and 1.10%(n=6)，respectively.Conclusion，the results were consistent for determination of ammonium alkaloids by the two methods，and filtrate method was comparatively simple.
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备注/Memo
第一作者简介：马茜（1988-），女，宁夏贺兰人，硕士，助理实验师，研究方向为生物学化学与分子生物学。E-mail:maqian226@126.com.基金项目：国家科技支撑计划子课题资助项目(2009BAI72B03)。