ZHANG Lilei,LI Guirong,LOU Tengxue,et al.Effect of Different Treatments on Pollen Viability of ‘Floribunda Roses’[J].Northern Horticulture,2015,39(16):72-95.[doi:10.11937/bfyy.201516018]
不同处理对“丰花月季”花粉生活力的影响
- Title:
- Effect of Different Treatments on Pollen Viability of ‘Floribunda Roses’
- 文献标志码:
- A
- 摘要:
- 以“丰花月季”品种为试材，采用正交实验设计，研究蔗糖、硼酸、CaCl2、MgSO4、ZnSO4 5个因素不同浓度对其花粉生活力的影响，以期找出最适宜“丰花月季”花粉萌发的培养条件。结果表明：蔗糖浓度对“丰花月季”花粉萌发的影响最大，其余依次为硼酸浓度、ZnSO4浓度、CaCl2浓度，MgSO4浓度的影响最小。一定浓度的蔗糖、硼酸、CaCl2对花粉的萌发有较大的促进作用，但超过一定浓度时起抑制作用，而ZnSO4浓度对花粉的萌发率影响是在一定范围内先降低后升高，MgSO4浓度对花粉的萌发影响不太明显。最适宜“丰花月季”花粉萌发的离体条件是200 g/L蔗糖+100 mg/L 硼酸+50 mg/L CaCl2+20 mg/L MgSO4+40 mg/L ZnSO4。
- Abstract:
- Taking‘Floribunda Roses’as experimental materials，the influence of five factors on pollen viability by orthogonal experiment design were discussed，and the five factors were the concentrations of sucrose，boric acid，CaCl2，MgSO4 and ZnSO4，so as to find out the best matching，namely，the optimum culture conditions of ‘Floribunda Roses’ pollen germination.The results showed that the concentration of sucrose had the greatest influence on ‘Floribunda Roses’ pollen germination，the concentrations of boric acid，ZnSO4 and CaCl2 had greater influence，and that the concentration of MgSO4 had minimal impact.A certain concentrations of sucrose，boric acid and CaCl2 for pollen germination had great role in promoting，but when the factors exceeded a certain concentration，they would have inhibitory effect.The effect of the concentration of ZnSO4 on pollen germination rate was later increased after the firstly decreased within a certain range.The concentration of MgSO4 had little effect on pollen germination.200 g/L sucrose+100 mg/L boric acid+50 mg/L CaCl2 +20 mg/L MgSO4 +40 mg/L ZnSO4 was the best in vitro culture medium for‘Floribunda Roses’pollen germination.
参考文献/References:
[1]李桂荣,杨鹏鸣.硼酸与蔗糖对百合花粉生活力的影响[J].安徽农业科学,2010,38(18):9494-9495. [2]尹佳蕾,赵惠恩.花粉生活力影响因素及花粉贮藏概述[J].中国农学通报,2005,21(4):110-113. [3]赵兴华,张道旭,印东生,等.百合花粉生活力测定和贮藏方法研究[J].北方园艺,2009(2):172-175. [4]周怀军,安连荣,朱哲锋.杏树不同品种及果枝花粉发芽试验报告[J].河北林果研究,2000,15(3):253-256. [5]黄家兴,吴杰,安建东,等.凯特杏花粉的离体培养及影响因子分析[J].西北植物学报,2008,28(1):52-58. [6]杨贵,罗慈文,张映南,等.不同蔗糖浓度对辣椒花粉萌发的影响[J].辣椒杂志,2006(3):24-26. [7]初庆刚.蔗糖、硼对樱桃属植物花粉萌发的影响[J].莱阳农学院学报,1995(3):21-13. [8]周旭红,桂敏,莫锡君.香石竹花粉生活力的研究[J].西南师范大学学报(自然科学版),2008,33(5):108-111. [9]RIHOVA L,HRABETOVA E,TUPY J.Optimization of conditions for in vitro pollen germination and tube growth in potatoes[J].International Journal of Plant Science,1996,157(5):561-566. [10]WANG Q,LU L,WU X,et al.Boron influences pollen germination and pollen tube growth in Piceameyeri[J].Tree Physiology,2003,23:345-351. [11]潘瑞炽.植物生理学[M].北京:高等教育出版社,2001. [12]沈捷,祝晨辰,徐进,等.硼、钙离子对杉木花粉萌发和花粉管生长影响[J].林业科技开发应用研究,2010,24(6):57-60. [13]屈海泳,刘连妹,孔文涛,等.桃花粉活性检测和离体萌发特性的研究[J].中国农学通报,2010(21):207-212. [14]张绍玲,陈迪新,康琅,等.培养墓组分及pH值对梨花粉萌发和花粉管生的影响[J].西北植物学报,2005,25(2):225-230. [15]董晓芬,郑剑宇.硼酸和钙离子对红叶李花粉萌发影响[J].大众技术,2007(5):134-135. [16]HAO J,YU F L,LIANG S P,et al.Changes of calcium distribution in egg cells，zygotes and two celled proembryos of rice(Oryza sativa L.)[J].Plant Report,2002,4:331-337. [17]龚明,曹宗馔.钙和钙调素对花粉萌发和花粉管生长的调控[J].植物生理学通讯,1995(5):321-328. [18]王岚岚,游捷,俞红强.月季花粉离体萌发液体培养基组分的优化[J].河北农业大学学报,2008,31(3):41-45. [19]李春林,杨水平,姚小华,等.普通油茶无性系花粉离体萌发特性[J].林业科学研究,2009(3):253-256.
相似文献/References:
[1]冯莎莎,姚太梅,刘 畅.冀西北地区日光温室条件下甜樱桃花粉生活力和花粉行为的研究[J].北方园艺,2014,38(03):44.
FENG Sha-sha,YAO Tai-mei,LIU Chang.Study on Pollen Viability and Pollen Performance of Sweet Cherry in Solar Greenhouse in the West-northern Part of Hebei[J].Northern Horticulture,2014,38(16):44.
[2]于海霞,崔路路,张 爽,等.不同贮藏方法对黄瓜花粉活力的影响[J].北方园艺,2014,38(03):107.
YU Hai-xia,CUI Lu-lu,ZHANG Shuang,et al.Effect of Different Storage Methods on Pollen Viability of Cucumber[J].Northern Horticulture,2014,38(16):107.
[3]谢焰锋,许林,戢小梅,等.茶花花粉生活力及贮藏力的研究[J].北方园艺,2013,37(02):74.
XIE Yan-feng,XU Lin,JI Xiao-mei,et al.Study on Pollen Vitality and Storage Capacity of Camellia japonica L.[J].Northern Horticulture,2013,37(16):74.
[4]吴月亮,赵传统,伊文芝,等.不同培养基及贮藏条件对文冠果花粉生活力的影响[J].北方园艺,2015,39(05):64.[doi:10.11937/bfyy.201505022]
WU Yue-liang,ZHAO Chuan-tong,YI Wen-zhi,et al.Effect of Pollen Viability of Xanthoceras sorbifolia Under Different Culture Media and Storage Conditions[J].Northern Horticulture,2015,39(16):64.[doi:10.11937/bfyy.201505022]
[5]刘杜玲,张博勇,彭少兵,等.贮藏温度及时间对核桃品种花粉生活力的影响[J].北方园艺,2015,39(16):7.[doi:10.11937/bfyy.201516002]
LIU Duling,ZHANG Boyong,PENG Shaobing,et al.Effect of Different Storage Temperature and Time on Pollen Viability of Walnut Cultivars[J].Northern Horticulture,2015,39(16):7.[doi:10.11937/bfyy.201516002]
[6]刘 伟,丁长春,常 征,等.淡黄花百合花粉离体保存条件研究[J].北方园艺,2015,39(23):108.[doi:10.11937/bfyy.201523030]
LIU Wei,DING Changchun,CHANG Zheng,et al.Study on Pollen Storage Condition of Lilium sulphureum Baker[J].Northern Horticulture,2015,39(16):108.[doi:10.11937/bfyy.201523030]
[7]陈梦华,赵丹,靳丽鑫,等.麻核桃花粉生活力与柱头可授性对坐果的影响[J].北方园艺,2016,40(03):28.[doi:10.11937/bfyy.201603008]
CHEN Menghua,ZHAO Dan,JIN Lixin,et al.Effect of Pollen Viability and Stigma Receptivity of Juglans hopeiensis Hu to Fruit Setting[J].Northern Horticulture,2016,40(16):28.[doi:10.11937/bfyy.201603008]
[8]王燕,赵鑫鑫,张志晓,等.六个苹果品种花粉生活力研究[J].北方园艺,2016,40(13):17.[doi:10.11937/bfyy.201613005]
WANG Yan,ZHAO Xinxin,ZHANG Zhixiao,et al.Study on Pollen Viability of Six Apple Cultivars[J].Northern Horticulture,2016,40(16):17.[doi:10.11937/bfyy.201613005]
[9]张义,张慧,汪凤威,等.“空心李”的授粉生物学特征[J].北方园艺,2017,41(24):43.[doi:10.11937/bfyy.20172194]
ZHANG Yi,ZHANG Hui,WANG Fengwei,et al.Pollination Biology Characteristics of Prunus salicina Lindl.cv.‘Kongxinli’[J].Northern Horticulture,2017,41(16):43.[doi:10.11937/bfyy.20172194]
[10]苗阳,董胜君,吴月亮,等.野杏花粉离体培养条件筛选及花粉生活力研究[J].北方园艺,2018,42(04):26.[doi:10.11937/bfyy.20173199]
MIAO Yang,DONG Shengjun,WU Yueliang,et al.Selection of Pollen Culture in vitro Conditions and Pollen Viability in Armeniaca vulgaris var .ansu[J].Northern Horticulture,2018,42(16):26.[doi:10.11937/bfyy.20173199]
备注/Memo
第一作者简介：张立磊（1978-），女，硕士，讲师，研究方向为园林植物资源研究及设计。E-mail：366893563@qq.com. 责任作者：李桂荣（1974-），女，回族，博士，副教授，现主要从事园艺植物育种及生物技术教学与科研工作。E-mail：liguirong10@163.com. 基金项目：河南省科技厅资助项目（143400410043）。