QI Hexing,JIA Qian,GAO Yuan,et al.Diversity and Distribution of Endophytic Fungi From Lycium barbarum L. of Ningxia[J].Northern Horticulture,2015,39(13):153-157.[doi:10.11937/bfyy.201513044]
宁夏枸杞果内生真菌多样性及其分布
- Title:
- Diversity and Distribution of Endophytic Fungi From Lycium barbarum L. of Ningxia
- 文章编号:
- 1001-0009（2015）13-0153-05
- 关键词:
- 枸杞果; 内生真菌; 相对频率; 多样性; rDNA-ITS序列
- 分类号:
- S 793.9
- 文献标志码:
- A
- 摘要:
- 以枸杞为试材，采用组织匀浆法从不同生长期的枸杞果中分离内生真菌，根据培养性状、菌落、孢子等的形态特征和rDNA-ITS序列分析，对分离菌株进行鉴定；根据枸杞果内生真菌的定殖率、相对频率、物种多样性指数、丰富度指数和相似性系数分析其组成特点。结果表明：从枸杞果中分离得到29株内生真菌，这些菌株分属于链格孢属（Alternaria）、枝顶孢属（Acremonium）、帚枝霉属（Sarocladium）、亚隔孢壳属（Didymella）、派伦霉属（Peyronellaea）、茎点霉属（Phoma）和盘二孢属（Marssonia） 7个属；其中链格孢属（Alternaria）、枝顶孢属（Acremonium）和帚枝霉属（Sarocladium）为优势属,分别占总菌株数的31.0%、27.6%和27.6%；成熟期枸杞果内生真菌物种多样性指数最大，幼果期枸杞果内生真菌物种多样性指数最小。枸杞果内含有丰富的内生真菌资源，成熟期枸杞果内生真菌多样性指数和丰富度指数远远高于幼果期和变色期。
- Abstract:
- Taking Lycium barbarum L.as test material，endophytic fungi were separated from Lycium barbarum L.which were different growing period by tissue homogenate.They were classified according to their morphological characteristics of culture，colonies and spores and rDNA-ITS sequences analysis.The composition characteristics of endophytic fungi were analyzed according to the Colonization rate(CR)，Relative frequency(RF)，Shannon-wiener index(H)，Margalef index(D) and Sorenson’s similarity coefficient(CS).The results showed that 29 endophytic fungi were isolated from Lycium barbarum L.，and they belong to Alternaria,Acremonium, Sarocladium, Didymella, Peyronellaea, Phoma and Marssonia respectively.Alternaria,Acremonium and Sarocladium were dominant genus and their Relative frequency (RF) was 31.0%，27.6% and 27.6% respectively.Shannon-wiener index (H) of endophytic fungi of mature period Lycium barbarum L.was most significance and least in young fruit period.And Lycium barbarum L.had abundant resources of endophytic fungi，Shannon wiener index(H) and Margalef index(D) of endophytic fungi of mature period Lycium barbarum L.were far more than ones of young fruit period and color change period.
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备注/Memo
第一作者简介：祁鹤兴（1990-），女，青海西宁人，硕士研究生，研究方向为生物防治及微生物资源利用。E-mail:qhx390495559@126.com.责任作者：顾沛雯（1969-），女，宁夏银川人，博士，教授，现主要从事植物病理学和生物防治及微生物资源利用等教学与科研工作。E-mail：gupeiwen2013@126.com.基金项目：国家自然科学基金资助项目（31460484）。