WANG Guiying,LIU Xiaojie,LI Shanshan,et al.Establishment of Populus deltoids cv.JuBa Regeneration System and Its Resistance to Hygromycin[J].Northern Horticulture,2015,39(13):98-102.[doi:10.11937/bfyy.201513029]
巨霸杨组培再生体系的建立及潮霉素抗性试验
- Title:
- Establishment of Populus deltoids cv.JuBa Regeneration System and Its Resistance to Hygromycin
- 文章编号:
- 1001-0009（2015）13-0098-05
- 分类号:
- S 792.11
- 文献标志码:
- A
- 摘要:
- 以巨霸杨为试材，采用不同植物激素组合及潮霉素不同浓度梯度筛选法，研究了巨霸杨组培再生体系建立及潮霉素对叶片和茎尖苗生长分化的影响。结果表明：诱导叶片芽分化的最佳培养基为MS+6-BA 0.25 mg/L+IBA 0.1 mg/L，再生芽在1/2MS+IBA 0.3 mg/L上生根形成完整植株。通过对无菌苗叶片和茎尖材料不同浓度梯度潮霉素抗性筛选试验，发现潮霉素（Hygromycin）对巨霸杨产生了较为敏感的选择压力，随着培养天数的增加潮霉素的抑制作用越来越明显。同茎尖苗比，叶片对潮霉素更为敏感。叶片培养2 d后即有明显受损表现，茎尖苗在培养4 d后才出现损伤。14 d后，在4 mg/L潮霉素条件下只有个别叶片在叶柄基部有少量芽分化；6 mg/L以上潮霉素条件下叶片及茎尖苗则全部失绿死亡。经过对比，确定巨霸杨的潮霉素筛选临界浓度范围为4~6 mg/L。该研究为巨霸杨的基因转化奠定了技术基础。
- Abstract:
- Taking a hybrid poplar variety Populus deltoids cv.JuBa (Populus deltoids 50×P.deltoides 36) as experiment material，the effect of different phytohormone combination and hygromycin concentration gradient on leaves and shoot tips were studied.The results showed that the optimal medium for bud induction was MS+6 BA 0.25 mg/L+IBA 0.1 mg/L，the optimal regeneration and rooting media was 1/2MS+IBA 0.3 mg/L.Through screening different hygromycin concentrations for leaves and shoot tips，hygromycin could affect the growth of JuBa poplar.The hygromycin inhibitory effects increased with growth time.Leaves were more sensitive comparing to shoot tips.After 2 days of cultivation，leaves were obviously damaged.The damage of shoot tips began to show only after 4 days.After 2 weeks，only a few buds appeared on petiole on 4 mg/L hygromycin；all leaves and shoot tips were dead under above 6 mg/L of hygromycin.The results demonstrated that the range of hygromycin critical concentration was from 4 mg/L to 6 mg/L.This research laid a technological foundation for the genetic transformation of this poplar variety.
参考文献/References:
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备注/Memo
第一作者简介：王桂英（1969-），女，博士，高级林业工程师，现主要从事植物分子生物学等研究工作。E-mail：moonlight0808@163.com.责任作者：杨敏生（1962-），男，博士，教授，现主要从事林木遗传育种等研究工作。E-mail：yangms100@126.com.基金项目：国家高技术研究发展计划（”863“计划）资助项目(2011AA100201)；国家自然科学基金资助项目（31370663）。