[1]李晓东,樊军锋,邱兴,等.外源Ca2+对低温胁迫下杨树幼苗抗寒相关生理指标的影响[J].北方园艺,2015,39(11):55-59.[doi:10.11937/bfyy.201511015]
　LI Xiao-dong,FAN Jun-feng,QIU Xing,et al.Effect of Exogenous Ca2+on Physiological Indexes inPoplar Seedlings Under Low Temperature Stress[J].Northern Horticulture,2015,39(11):55-59.[doi:10.11937/bfyy.201511015]

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外源Ca²⁺对低温胁迫下杨树幼苗抗寒相关生理指标的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷期数: 2015年11 页码: 55-59 栏目: 出版日期: 2015-06-15

Title:: Effect of Exogenous Ca²⁺on Physiological Indexes inPoplar Seedlings Under Low Temperature Stress

文章编号:: 1001-0009(2015)11-0055-05

作者:: 李晓东¹; 樊军锋¹; 邱兴¹; 张耀宏²; 史禹博³; 1.西北农林科技大学林学院，陕西杨凌 712100；2.国家林业局西北林业调查规划设计院，陕西西安 710048；3.常山油茶研究所，浙江常山 324200

Author(s):: LI Xiao-dong¹; FAN Jun-feng¹; QIU Xing¹; ZHANG Yao-hong²; SHI Yu-bo³; .College of Forestry，Northwest Agricultural and Forest University，Yangling，Shaanxi 712100；2.Northwest Institute of Forest Inventory，Planning and Design，Xi’ an，Shaanxi 710048；3.Changshan Tea Oil Research Institution，Changshan，Zhejiang 324200

关键词:: 杨树; Ca²⁺; 低温胁迫; 抗寒性; 膜伤害率

Keywords:: poplar; Ca²⁺; low temperature stress; cold tolerance; membrane injury rate

分类号:: S 792.11

DOI:: 10.11937/bfyy.201511015

文献标志码:: A

摘要:: 以“陕林4号”和“07-69×青1”为试材，分别用浓度为0、5、10、15、20 mmol/L的CaCl₂溶液喷施杨树幼苗，4℃低温胁迫2 d后测定杨树幼苗的膜伤害率、丙二醛(MDA）含量、抗氧化酶(SOD和POD）活性以及可溶性蛋白质含量等生理指标，以探究低温胁迫下不同浓度外源Ca²⁺对杨树幼苗抗寒相关生理指标的影响。结果表明：在喷施10 mmol/L CaCl₂的作用下，杨树幼苗的各项抗寒相关生理指标最优，可显著降低杨树幼苗的膜伤害率，减少丙二醛(MDA）含量，提高超氧化物歧化酶(SOD）活性和过氧化物酶(POD）活性，增加可溶性蛋白质含量。外源Ca²⁺可以有效提高杨树幼苗的抗寒能力，缓解低温胁迫对杨树幼苗的伤害。

Abstract:: The young seedlings of ‘Shaanlin No4’ and‘07-69×P.cathayana 1’ were used as experiment materials.In this research，CaCl₂ concentrations of 0，5，10，15，20 mmol/L were sprayed before treated under 4℃ stress for 2 days.Then，physiological indexes including membrane injury rate，activities of antioxidant enzymes (SOD and POD)，contents of MDA and soluble protein were measured to investigate the effect of different Ca²⁺concentration on cold tolerance related physiological indexes in poplar under low temperature stress.The results showed that membrane injury rate and MDA content were decreased under 10 mmol/L CaCl₂ treatment，while antioxidant enzymes (SOD and POD) and soluble protein were increased significantly.In conclusion，it was made sense that exogenous Ca²⁺could effectively improve the cold tolerance of poplar seedlings and alleviate the damage caused by low temperature stress.

参考文献/References:

[1] 沈漫,王明庥,黄敏仁.植物抗寒机理研究进展[J].植物学通报,1997,14(2):1-8.

[2] 李美如,刘鸿先,王以柔.植物细胞中的抗寒物质及其与植物抗冷性的关系[J].植物生理学通讯,1995,31(5):328-334.[3] 樊军锋,周永学,高建社,等.陕西杨树育种历史及展望[J].西北林学院学报,2004,19(2):77-81.[4] RickauerM,Taner W.Effects of Ca²⁺on amino acid transport and accumulation in roots of Phaseolus vulgaris[J].Plant Physiology,1986,82(1):41-46.[5] Kitagawa Y,Yoshizaki K.Water stress-induced chilling tolerance in rice：putative relationship between chilling tolerance and Ca²⁺flux[J].Plant Science,1998,137(1):73-85.[6] 李卫,孙中海,章文才,等.钙与钙调素对柑橘原生质体抗冻性的影响[J].植物生理学报,1997,23(3):262-266.[7] 赫春长.外源Ca对低温下麻风树生理生化调节效应的研究[D].福州:福建农林大学,2010.[8] 梁颖,王三根.Ca2+对水稻种子活力和抗寒力的影响[J].西南农业大学学报,1996,18(5):491-495.[9] 李新国,张建霞,孙中海.Ca²⁺信号系统对低温下柑橘膜脂过氧化和抗氧化酶的影响[J].广西植物,2007,27(4):643-648.[10]耿广东,程智慧,张素勤.外源Ca²⁺对茄子幼苗抗寒性的影响[J].西南农业大学学报(自然科学版),2006,28(3):432-435.[11]冀乙萌,惠杜鹃,李瑞梅,等.钙对低温胁迫下木薯抗寒相关生理指标的影响[J].热带作物学报,2012,33(5):894-898.[12]林善枝,蔡世英,陈晓敏.低温锻炼对香蕉幼苗钙调蛋白含量及其可能调节酶类活性的影响[J].热带作物学报,2001,22(4):29-35.[13]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000. [14]白建军.外源物质对大扁杏花器抗寒性的影响[J].西北林学院学报,2008,23(1):82-86.[15]康国章,陶均,孙谷畴,等.H₂O₂和Ca²⁺对受低温胁迫香蕉幼苗抗冷性的影响[J].园艺学报,2002,29(2):119-122.[16]Monroy A F,Dhindsa R S.Low temperature signal transduction：induction of cold acclimation-specific genes of alfalfa by calcium at 25℃[J].Plant Cell,1995,7(3):321-331.[17]Yang T,Poovaiah B W.Calcium/calmodulin-mediated signal network in plants[J].Trends in Plant Science,2003,8(10):505-512.[18]张燕,方力,李天飞,等.钙对低温胁迫的烟草幼苗某些酶活性的影响[J].植物学通报,2002,19(3):342-347.

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备注/Memo

第一作者简介：李晓东(1991-），男，硕士研究生，研究方向为林业生物技术。E-mail：lixiaodong268@163.com.责任作者：樊军锋(1963-），男，博士，研究员，硕士生导师，现主要从事杨树良种选育和油松遗传改良等研究工作。E-mail：fanjf28@163.com. 基金项目：国家林业局林业公益性行业科研专项资助项目(201404113）。

更新日期/Last Update: 2015-08-06