[1]孙庆扬,韩宁,孙玉霞,等.疏粒处理对“赤霞珠”葡萄果实含糖量及相关代谢酶活性的影响[J].北方园艺,2015,39(09):18-22.[doi:10.11937/bfyy.201509005]
　SUN Qing-yang,HAN Ning,SUN Yu-xia,et al.Effect of Berry-thinning on Sugar Content and Relative Enzyme Activities During Berry Ripening in ‘Cabernet Sauvignon’ Grape[J].Northern Horticulture,2015,39(09):18-22.[doi:10.11937/bfyy.201509005]
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疏粒处理对“赤霞珠”葡萄果实含糖量及相关代谢酶活性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年09 页码: 18-22 栏目: 出版日期: 2015-05-15
Title:
Effect of Berry-thinning on Sugar Content and Relative Enzyme Activities During Berry Ripening in ‘Cabernet Sauvignon’ Grape
文章编号:
1001-0009(2015)09-0018-05
作者:
孙庆扬1; 韩宁1; 孙玉霞2; 赵悦1; 韩爱芹1; 赵新节1
（1.齐鲁工业大学 山东省微生物工程重点实验室，山东 济南 250353；2.山东省农业科学院 农产品研究所，山东 济南 250100）
Author(s):
SUN Qing-yang1; HAN Ning1; SUN Yu-xia2; ZHAO Yue1; HAN Ai-qin1; ZHAO Xin-jie1
（1.Shandong Key Laboratory of Microbial Engineering，Qilu University of Technology，Jinan,Shandong 250353；2.Institute of Agro-food Science and Technology，Shandong Academy of Agricultural Sciences，Jinan，Shandong 250100)
关键词:
“赤霞珠”葡萄; 疏粒; 酸性转化酶; 叶片可溶性糖; 果实含糖量
Keywords:
‘Cabernet Sauvignon’grape; berry-thinning; acid invertase; soluble sugar content of leaf; sugar content of grape berries
分类号:
S 663.1
DOI:
10.11937/bfyy.201509005
文献标志码:
A
摘要:
以酿酒葡萄品种“赤霞珠”为试材，分析了不同疏粒量处理对始熟期后葡萄叶片内容物、果实大小、果实糖酸含量及果实酸性转化酶活性的影响。结果表明：从始熟期开始随着果实成熟，对照和处理的果实纵横径及糖含量均逐渐增加，而果实酸含量及叶片中可溶性糖含量均逐渐降低；与此同时，可溶性酸性转化酶活性都呈先降低后升高趋势而细胞壁酸性转化酶活性却持续升高。另一方面，在相同果实成熟期随疏粒量的增加，果实糖含量明显上升，酸含量明显下降，而可溶性酸性转化酶及细胞壁酸性转化酶的活性均升高。另外，疏粒处理对各时期果实纵横径、叶片叶绿素影响不大，但随疏粒量的增加，各时期叶片可溶性糖含量却逐渐降低。对果实酸性转化酶活性、叶片可溶性糖及果实含糖量进行相关性分析发现，细胞壁酸性转化酶活性与叶片可溶性糖含量呈显著负相关而与果实含糖量呈显著正相关，而且随着果实疏粒量的增加，相关性有升高趋势。总之，葡萄果实成熟期疏粒处理对葡萄果实中的酸性转化酶产生一定影响，尤其是细胞壁酸性转化酶，最终可能改变了葡萄树体的库源关系，增加了果实库强，使更多的碳水化合物分配到果实中。
Abstract:
Taking grape variety ‘Cabernet sauvignon’ as materials，the ingredient content of leaf，the fruit diameter，acid and sugar content and activities of acid invertase in berry of ‘Cabernet Sauvignon’ during fruit ripening of different degree by berry-thinning were studied.The results showed that whether berry-thinning or not，with berry ripening fruit diameter and sugar content in grape berries increased，while acid content of fruit and soluble sugar content of leaf decreased.And meanwhile acid invertase activity increased gradually with berry ripening.Furthermore，with an increasing degree of berry-thinning，the activities of soluble acid invertase and cell wall-bound acid invertase were enhanced obviously，in agreement with increasing in content of fruit sugar.However，the berry-thinning treatment had little effect on diameter of fruit and chlorophyll content of leaf，but the soluble sugar content of leaf decreased significantly in berry-thinning treatment compared with control.Correlation analysis showed the activity of cell wall-bound acid invertase was negative correlation with the soluble sugar content of leaf and was positive correlation with the sugar content of grape berries.Moreover，the correlation coefficient was higher with an increasing degree of berry-thinning.The results suggested that berry-thinning treatment had great influence on the acid invertase of fruit，especially cell wall-bound acid invertase in the berry mature period.And it might increase the fruit sink strength，and then change the relationship of sink and source therefore，more carbohydrate were allocated to fruit.

参考文献/References:


[1]Devainh K A，HemanthV H，Sheikh M B.Relationship between acid invertase activity and sugar content in grape species[J]. Journal of Food Biochemistry ，2011，35:1646-1652.
[2]张晓燕.葡萄果实从成长到成熟的转折伴随着韧皮部卸载路径的转变[D].北京:中国农业大学，2006.
[3]张家荣.负载量及其调控方式对酿酒葡萄果实中花色苷的影响[D].济南:齐鲁工业大学，2013.
[4]Kaps M L，Cahoon G A.Berry thinning and cluster thinning influence vegetative growth，yield，fruit composition，and net photosynthesis of ‘Seyval blanc’ grapes[J].Journal of the American Society for Horticultural Science，1989，114:20-24.
[5]刘品何,刘胜,秦伟帅,等.疏果方式对”赤霞珠”葡萄挥发性物质的影响[J].北方园艺，2014(9)：27-32.
[6]谢兆森.根域限制对葡萄果实发育、源库器官及其输导组织结构的影响[D].上海:上海交通大学，2010
.[7]Miron D，Schaffer A A.Sucrose phosphate synthase,sucrose synthase and invertase activities in developing fruit of Lycopersicon esculeutum Mill，and sucrose accumulation in Lycopersion hirsutum Humb and bonpl[J]. Plant Physiology,1991,95:623-627.
[8]王永章,张大鹏.果糖和葡萄糖参与诱导苹果果实酸性转化酶翻译后的抑制性调节[J].中国科学(C辑)，2002，32(1)：32-39.
[9]刘品何.疏果对酿酒葡萄果实及其葡萄酒挥发性物质的影响[D].济南:齐鲁工业大学，2014.
[10]陈俊伟,张上隆,张良诚.果实中糖的运输、代谢与积累及其调控[J].植物生理与分子生物学学报，2004，30(1):1-10.
[11]林玲,孙光明,李绍鹏,等.园艺植物果实中糖代谢的研究进展[J].华南热带农业大学学报，2005，11(4):37-41.
[12]卢彩玉,郑小艳,贾惠娟,等.根域限制对“巨玫瑰”葡萄果实可溶性糖含量及相关代谢酶活性的影响[J].园艺学报,2011,38(5):825-832.
[13]闫梅玲,王振平,范永,等.蔗糖代谢相关酶在赤霞珠葡萄果实积累中的作用[J].果树学报，2010，27(5):703-707.
[14]Pan Q H，Cao P，Duan C Q.Comparison of enzymes involved in sugar metabolism from Shang-24(Vinifera quinguangularis)and Cabernet Sauvignon(Vinifera vinifera) atveraison[J].Australian Journal of Grape and Wine Research，2008，15：9-17.
[15]刘淑慧,侯智霞.喷施蔗糖对蓝莓叶片和果实中可溶性糖含量变化的影响[J].安徽农业科学，2012，40(16)：8981-8984.
[16]Marcelis L F M.Effect of assimilate supply on the growth of individual cucumber fruits[J].Physiologiapycnantha，2006，87:1781-1791.
[17]Zhang D P.Auxin treatment of pre-veraison grape (Vitis vinifera L.) berries both delays ripening and increases the synchronicity of sugar accumulation[J].Australian Journal of Grape and Wine Research，2010，17:1-8.
[18]Frommer W B，Sonnewald U.Molecular analysis of carbon partitioning in solanaceousspecies[J].Journal of Experimental Botany，1995，46:587-607.
[19]Patrck J W.Phloem unloading：Sieve element unloading and post-sieve element transport[J].Annual Review of Plant Biology，1997，48:191-222.
[20]Matthews M A，Thomas T R，Shackel K A.Fruit ripening in Vitis vinifera L.：possible relation of veraison to turgor and berry softening[J].Australian Journal of Grape and Wine Research，2009，15:278-283.
[21]Thomas T R，Shackel K A，Matthews M A.Mesocarp cell turgor in Vitis vinifera L.berries throughout development and relation to firmness，growth，and the onset of ripening[J].Planta，2008，228:1067-1076.

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备注/Memo

第一作者简介：孙庆扬（1988-），女，硕士研究生，研究方向为葡萄与葡萄酒。E-mail:1009058695@qq.com.

责任作者：赵新节（1962-），男，博士，教授，硕士生导师，研究方向为葡萄与葡萄酒。E-mail：zhaoxinjie1177@163.com
.基金项目：山东省科技发展计划资助项目（2012GGB01059）；山东省自然科学基金资助项目（ZR2013CQ022）；山东省现代农业产业技术体系专项基金资助项目。

更新日期/Last Update: 2015-08-01