ZHOU Jia-hong,XING Cai-hua,CAO Hui,et al.Study on Regeneration of Thin Cell Layer of Pyrus ussuriensis[J].Northern Horticulture,2015,39(06):95-98.[doi:10.11937/bfyy.201506026]
秋子梨茎段薄层细胞培养再生初探
- Title:
- Study on Regeneration of Thin Cell Layer of Pyrus ussuriensis
- Keywords:
- Pyrus ussuriensis; thin cell layer; regeneration; stem
- 文献标志码:
- A
- 摘要:
- 以秋子梨试管苗为试材,进行了其茎段薄层细胞组织培养再生体系的构建,以期为秋子梨无性繁殖和工厂化育苗奠定基础,同时为薄层细胞培养在梨属植物当中的应用提供参考。结果表明:秋子梨茎段薄层细胞的再生受到激素浓度和配比的影响,其中不定芽再生最佳培养基为MS+BA 1.5 mg/L+NAA 0.25 mg/L+蔗糖30 g/L+琼脂糖7 g/L,再生效率达100%;不定根再生最佳培养基为1/2MS+IBA 0.5 mg/L+蔗糖30 g/L+琼脂糖7 g/L,根再率达66.7%;同时研究还发现适宜的暗培养有助于愈伤组织的诱导和不定芽的再生。
- Abstract:
- Regeneration system of thin cell layers (TCL)was established by using shoots of Pyrus ussuriensis as explants,to provide basis for asexual propagation and industrialized seedling production of Pyrus ussuriensis,meanwhile to provide reference for application of TCL technique in Pyrus research.The results showed that regeneration efficiency of TCL was affected by the combination and concentration of hormones.The optimal culture medium of adventitious shoots regeneration was MS+BA 1.5 mg/L+NAA 0.25 mg/L+sucrose 30 g/L+agarose 7 g/L,and the regeneration rate was 100%.The optimal culture medium of adventitious roots was 1/2MS+IBA 0.5 mg/L+sucrose 30 g/L+agarose 7 g/L,and the rate of rooting was 66.7%.Meanwhile,callus and adventitious shoots induction rate increased under dark culture.
参考文献/References:
[1]张绍玲.梨学[M].北京:中国农业出版社,2013:48-66. [2]李嫦艳,吴翠云.新梨七号茎段组织培养再生植株的研究[J].西北农业学报,2011,20(5):130-134. [3]韩文璞,袁明莲.黄金梨的组织培养和快繁研究[J].落叶果树,2001,33(2):7-8. [4]张玉娇,蔺经,丛郁,等.黄冠梨组织培养与快繁技术研究[J].江苏农业科学,2009(3):35-36. [5]Tran Thanh van K M,Drira A.Definition of a simple experimental system of directed organogenesis de novo:Organ neoformation from epidermal tissue of Nautilocalyx lynchei[J].Les Cultures de Tissus des Plantes,1970,193:169-176. [6]王鸿鹤,黄学林.薄层培养的应用现状与前景[J].植物学通报,1999,16(6):631-635. [7]张虹.梨树组织培养研究的进展[J].广西热带农业,2004(5):12-16. [8]谭雪辉,刘洪章.梨组织培养与遗传转化研究进展[J].北方园艺,2007(1):147-149. [9]李晓刚,王宏伟,杨青松,等.豆梨低玻璃化组培快繁技术[J].江苏农业科学,2012,40(12):54-56. [10]王苏珂,杨健,王龙,等.西洋梨矮化砧木BA-29的组培快繁技术研究[J].果树学报,2010,27(6):1002-1005. [11]杨芳,王忆,许雪峰,等.秋子梨叶片植株再生研究[J].中国果树,2008(3):13-16. [12]张红晓,经剑颖.木本植物组织培养技术研究进展[J].河南科技大学学报,2003,23(3):66-69. [13]郑亚杰,张茂君,姚寰宇,等.山梨组织培养及快繁技术研究[J].吉林农业科学,2010,35(1):45-46. [14]刘利平.‘卡特’夏橙薄层细胞培养高频再生体系建立及转基因应用初探[D].武汉:华中农业大学,2008.
相似文献/References:
[1]梁 文 杰,谭 晓 风,王 立 新,等.秋子梨品种`龙香’ S 基因型的鉴定及梨S 42 -RNase新基因的序列分析[J].北方园艺,2009,33(12):0.[doi:10.11937/bfyy.2009012005]
LIANG Wen -jie,TAN Xiao -feng,et al.Identification of S-genotypes and Sequence Analyses of S 42 -R Nase from `LongXiang’( Pyrus ussuriensis)[J].Northern Horticulture,2009,33(06):0.[doi:10.11937/bfyy.2009012005]
[2]张 春 芳,李 茂 福,韩 振 海,等.秋子梨等九个品种 S 基因型的鉴定[J].北方园艺,2008,32(11):0.[doi:10.11937/bfyy.2008011051]
ZHANG Chun -fang,LI Mao -fu,HAN Zhen -hai,et al.Identification of S -genotypes of Ussurian Pear Cultivars[J].Northern Horticulture,2008,32(06):0.[doi:10.11937/bfyy.2008011051]
[3]刘 延 杰.秋子梨不同类型生长势与枝叶解剖构造的研究[J].北方园艺,2000,24(05):0.[doi:10.11937/bfyy.200005018]
[J].Northern Horticulture,2000,24(06):0.[doi:10.11937/bfyy.200005018]
[4]王强,卢明艳,武春昊,等.秋子梨亲本组配对F1早果性的影响[J].北方园艺,2021,(02):37.[doi:10.11937/bfyy.20201362]
WANG Qiang,LU Mingyan,WU Chunhao,et al.Effects of Parent Selection and Crosses of Pyrus ussuriensis on the Early-bearing Ability of F1[J].Northern Horticulture,2021,(06):37.[doi:10.11937/bfyy.20201362]
备注/Memo
第一作者简介:周佳红(1992-),女,山东烟台人,本科,研究方向为园艺植物逆境生理与分子生物学。E-mail:532003959@qq.com. 责任作者:李鼎立(1979-),男,博士,副教授,现主要从事果树分子生物学等研究工作。基金项目:山东省优秀中青年科学家科研奖励基金资助项目(BS2011NY007);现代农业产业技术体系建设专项资金资助项目(CARS-29);青岛农业大学高层次人才启动基金资助项目(630920)。