[1]盛春鸽,王延锋,潘春磊,等.糙皮侧耳杂交后代遗传多样性分析[J].北方园艺,2015,39(04):149-151.[doi:10.11937/bfyy.201504036]
　SHENG Chun-ge,WANG Yan-feng,PAN Chun-lei,et al.Analysis for Genetic Diversity of Pleurotus ostreatus Hybrids[J].Northern Horticulture,2015,39(04):149-151.[doi:10.11937/bfyy.201504036]

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糙皮侧耳杂交后代遗传多样性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷期数: 2015年04 页码: 149-151 栏目: 出版日期: 2015-02-28

Title:: Analysis for Genetic Diversity of Pleurotus ostreatus Hybrids

作者:: 盛春鸽; 王延锋; 潘春磊; 徐德海; 侯国强; （黑龙江省农业科学院牡丹江分院，黑龙江牡丹江 157041）

Author(s):: SHENG Chun-ge; WANG Yan-feng; PAN Chun-lei; XU De-hai; HOU Guo-qiang; (Mudanjiang Branch,Heilongjiang Academy of Agricultural Sciences，Mudanjiang，Heilongjiang 157041)

关键词:: 糙皮侧耳; 杂交后代; 遗传多样性

Keywords:: Pleurotus ostreatus; hybrid generation; genetic diversity

DOI:: 10.11937/bfyy.201504036

文献标志码:: A

摘要:: 以糙皮侧耳Pleurotus ostreatus白色菌株P1和深灰色菌株P2为亲本，通过单孢菌株配对杂交获得40个杂交组合。出菇记录杂交后代子实体颜色，提取42个样本的全基因组DNA，用ISSR进行遗传多样性分析。结果表明：6条ISSR引物共扩增出45条带，其中多态性条带34条，多态性比率为75.56%，有效等位基因数为N_e=1.4858，遗传多样性指数H=0.2725，香农信息指数I=0.4021。聚类分析后发现，在 0.6889水平上聚为4类,聚类结果与子实体颜色关系不大，表明糙皮侧耳杂交后代存在丰富的遗传变异。分类结果将有利于更好的利用这些丰富的育种资源。

Abstract:: White strain (P1) and dark grey strain (P2) of Pleurotus ostreatus were used as dikaryon parents for single-spore crossbreeding.40 inbred lines P1×P2 were constructed，fruit-body color of 40 combines were observed.40 offspring and 2 parents were identified for genetic diversity by ISSR technique.The results showed that，6 ISSR primers generated 45 fragments，polymorphic bands was 34,polymorphism ratio 75.56%,the effective number of alleles (Ne)=1.4858，Nei’s gene diversity (H)=0.2725，Shannon information index (I)=0.4021，UPGMA based clustering separated the 42 test strains into 4 groups at an overall similarity coefficient of 0.6889 which had no directly contacts with its fruit-body color.The results showed that，a high level of genetic variation in P.ostreatus inbred lines.It would be beneficial to use various target varieties to broaden the genetic background of P.ostreatus.

参考文献/References:

[1]马晓龙.糙皮侧耳优良杂交子筛选与侧耳属野生菌株驯化栽培研究[D].武汉：华中农业大学,2009. [2]黄毅.食用菌栽培[M].北京：高等教育出版社,2008：87-89. [3]林芳灿,汪中文,孙勇.中国香菇自然群体的交配因子分析[J].菌物系统,2003（22）：235-240. [4]黄建安,李家贤,黄意欢,等.茶树品种资源遗传多样性的AFLP研究[J].园艺学报,2006,33(2)：317-322. [5]范凯,洪永聪,丁兆堂,等.茶树‘黄山种’自然杂交后代遗传多样性分析[J].园艺学报,2010,37(8)：1357-1362. [6]李红梅,尚晓冬,谭琦,等.刺芹侧耳自交S1代若干性状的遗传分化[J].菌物学报,2009,28(4)：541-547. [7]韩国辉,向素琼,汪卫星,等.沙田柚杂交后代群体的SSR鉴定与遗传多样性分析[J].中国农业科学,2010,43(22)：4678-4686. [8]吕林兰,杜晓东,王嫣,等.马氏珠母贝3个野生种群及种群间杂交后代遗传多样性的ISSR分析[J].水生生物学报,2008,32(1)：26-32. [9]邓普恩,刘丽,刘楚吾.罗非鱼亲本与杂交子代遗传差异的RAPD分析[J].现代农业科学,2008,15(12)：95-98,111. [10]宫志远,任海霞,姚强,等.35个山东主栽平菇菌株的ISSR遗传差异分析[J].基因组学与应用生物学,2010,29(3)：507-512. [11]王卓仁,刘启燕,边银丙,等.香菇单孢杂交子代群体灰色关联度和ISSR分析[J].菌物学报,2010,29(2)：267-272. [12]张金霞,黄晨阳,管桂萍,等.白黄侧耳Pleurotus cornucopiae微卫星间区(ISSR)分析[J].菌物学报,2007,26(1)：115-121.

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备注/Memo

第一作者简介：盛春鸽（1985-），女，硕士，研究实习员，研究方向为食用菌遗传育种。E-mail：shengchunge@163.com.责任作者：王延锋（1973-），男，博士，研究员，现主要从事食（药）用菌遗传育种与栽培技术及产品保鲜精深加工等研究工作。E-mail：mdjnks@126.com.基金项目：黑龙江省应用技术研究与开发计划资助项目（WB13B10602）；牡丹江市科技攻关资助项目（Z2013n014）。

更新日期/Last Update: 2015-04-21