LIN Qin,AbudirehemuREHEMAN,REN Xian.Karyotype and Banding Pattern Analysis of Somatic Cell Chromosome in Astragalus mongolicus[J].Northern Horticulture,2015,39(04):104-107.[doi:10.11937/bfyy.201504024]
蒙古黄芪体细胞染色体核型及带型分析
- Title:
- Karyotype and Banding Pattern Analysis of Somatic Cell Chromosome in Astragalus mongolicus
- Keywords:
- Astragalus mongolicus; chromosome; karyotype analysis; C-banding
- 文献标志码:
- A
- 摘要:
- 以蒙古黄芪种子根尖为试验材料，采用细胞化学染色与制片、C-分带、计算机及其软件辅助，测量鉴定与比对分析的方法，研究了中药草蒙古黄芪体细胞染色体数目、核型与带型。结果表明：蒙古黄芪体细胞染色体核型是 2n=16=2L+4M1+6M2+4S，共有8对染色体，核型类型属于2B型，A.sK（核型不对称系数）=16.17/25.63×100%=63.09%，染色体总体积为69.0 μm3；蒙古黄芪的体细胞染色体C-带带型为2n=16=8m+6sm+2sm+sat，第3号染色体短臂区段带有随体，该试验可为黄芪及其蒙古黄芪的鉴定识别与利用提供参考。
- Abstract:
- Taking the root tip of seed from Astragalus mongolicus as material,the chromosome number,karyotype and zone type of somatic cell were studied by the methods of chemical staining,producer slide,C-banding methods and technology,computer and its software measurement and so on.The results showed that the number of RTC of Astragalus mongolicus was 8，and the karyotype formula was 2n=16=2L+4M1+6M2+4S.The karyotype belonged to 2B type；and A.sK (asymmetrical karyotype coefficient)=16.17/25.63×100%=63.09%.The total volume of chromosome was 69.0 μm3.In addition，the C-banding formula was 2n=16=8m+6sm+2sm+sat.There was a satellite in the segment of short arm of chromosome 3，which would lay the foundation for identifying and utilizing Astragalus membranaceus and Astragalus mongolicus.
参考文献/References:
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备注/Memo
第一作者简介：林勤（1964-），男，本科，高级工程师，研究方向为微生物发酵及农产品加工。E-mail：18709589588@163.com.责任作者：任贤（1957-），男，硕士，教授，研究方向为遗传育种。E-mail：renxian57@163.com.基金项目：国家民委《微生物发酵酿造工艺重点实验室》资助项目。