[1]王多佳,孙莉莉,黄莎莎,等.光质对矮化黄瓜膨胀素基因表达的影响[J].北方园艺,2015,39(02):84-88.[doi:10.11937/bfyy.201502024]
　WANG Duo-jia,SUN Li-li,HUANG Sha-sha,et al.Effect of Light Quality on the Expression of Expansin Gene in Dwarf Cucumber[J].Northern Horticulture,2015,39(02):84-88.[doi:10.11937/bfyy.201502024]
点击复制

光质对矮化黄瓜膨胀素基因表达的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年02 页码: 84-88 栏目: 出版日期: 2015-01-30
Title:
Effect of Light Quality on the Expression of Expansin Gene in Dwarf Cucumber
作者:
王多佳; 孙莉莉; 黄莎莎; 李凤兰; 胡宝忠
(东北农业大学 生命科学学院，黑龙江 哈尔滨 150030)
Author(s):
WANG Duo-jia; SUN Li-li; HUANG Sha-sha; LI Feng-lan; HU Bao-zhong
(Institute of Life Science，Northeast Agricultural University，Harbin,Heilongjiang 150030)
关键词:
光质; 膨胀素; 矮化; 表达
Keywords:
light quality; expansin; dwarf; expression
DOI:
10.11937/bfyy.201502024
文献标志码:
A
摘要:
以矮生黄瓜“D0462” 和蔓生黄瓜“129” 为试材，研究不同光质对其膨胀素基因表达的影响。结果表明:不同光质对黄瓜下胚轴的伸长起到或抑制或促进的作用;通过Real-time PCR定量分析推测红光抑制膨胀素基因的表达；蓝光对膨胀素基因Cs-EXPA1，Cs-EXPA2的表达先抑制后促进;推断黄瓜“D0462”的矮化性状与膨胀素基因表达关系密切，其中Cs-EXPA1基因的影响尤为关键。
Abstract:
Taking sprawl cucumber（Cucumis sativus L.）‘129’ and dwarf cucumber ‘D0462’ as experimental materials,the effects of different light qualities on expression of the expansin were studied.The results showed that the hypocotyls length of ‘D0462’ was longer or shorter by different light treatment.The expression of expansin was detection by Real-time PCR.The red light inhibited the expression of expansin and blue light inhibited at first but promoted after that.It was inferred that the dwarf character of‘D0462’was related to the expression of expansin,espcially with the Cs-EXPA1.

参考文献/References:

[1]Sampedro J，Cosgrove D J.The expansin superfamily[J].Genome Biology，2005,6(12)：242.

[2]Cosgrove D J.How do Plant cell wall extend?[J].Plant Physiology,1993,102：1-6.
[3]Sasidharan R，Chinnappa C C，Voesenek L A C J,et al.The regulation of cell wall extensibility during shade avoidance：a study using two contrasting ecotypes of Stellaria longipes[J].Plant Physiology,2008，148：1557-1569.
[4]孙莉莉,李凤兰,秦智伟,等.光质对矮生黄瓜生长及生理特性的影响[J].中国蔬菜,2011(8):65-70.
[5]张微慧,张光伦.光质对果树形态建成及果实品质的生理生态效应[J].中国农学通报,2007,23(1):78-83.
[6]崔瑾,马志虎,徐志刚,等.不同光质补光对黄瓜、辣椒和番茄幼苗生长及生理特性的影响[J].园艺学报,2009,36(5):663-670.
[7]杜健芳,廖祥儒,叶步青,等.光质对油菜幼苗生长及抗氧化酶活性的影响[J].植物学通报,2002(19):743-745.
[8]张瑞华,徐坤,董灿兴,等.光质对姜生长及光能利用特性的影响[J].园艺学报,2008,35(5):673-680.
[9]张欢,徐志刚,崔瑾,等.不同光质对萝卜芽苗菜生长和营养品质的影响[J].中国蔬菜,2009(10):28-32.
[10]Lin C.Blue light receptors and signal transduction[J].Plant Cell,2002,14:207-225.

相似文献/References:

[1]陈祥伟,刘世琦,刘 庆,等.不同LED光源对小白菜生长及光合特性的影响[J].北方园艺,2013,37(22):1.
　CHEN Xiang-wei,LIU Shi-qi,LIU Qing,et al.Effects of Different Light Qualities on Growth and Photosynthetic Characteristics of Pakchoi[J].Northern Horticulture,2013,37(02):1.
[2]孙文泰,尹晓宁,刘兴禄,等.不同海拔高度对“红富士”苹果果实品质的影响[J].北方园艺,2013,37(06):12.
　SUN Wen-tai,YIN Xiao-ning,LIU Xing-lu,et al.Effect of Different Altitudes on Quality of ‘Red Fuji’ Apple[J].Northern Horticulture,2013,37(02):12.
[3]郭君丽,王俊甫,蒋福稳,等.光质和2,4-D对47号怀山药叶片愈伤组织诱导形成的影响[J].北方园艺,2012,36(15):174.
　GUO Jun-li,WANG Jun-fu,JIANG Fu-wen,et al.Effects of Light Quality and 2,4-D Combination on Callus Inductionin Leaves of 47 Dioscorea opposita Thunb.[J].Northern Horticulture,2012,36(02):174.
[4]陈博,陈哲皓,王利琳,等.不同光质对喜树愈伤组织形成及分化潜质影响[J].北方园艺,2012,36(17):112.
　CHEN Bo,CHEN Zhe-hao,WANG Li-lin,et al.Effects of Different Light Quality on the Formation and Regenerative Potentialities of Calli of Camptotheca acuminata[J].Northern Horticulture,2012,36(02):112.
[5]孙雅洁,李明,李守勉,等.不同光质对杏鲍菇生长发育的影响[J].北方园艺,2014,38(19):142.
　SUN Ya-jie,LI Ming,LI Shou-mian,et al.Effect of Different Light Quality on the Growth of Pleurotus eryngii[J].Northern Horticulture,2014,38(02):142.
[6]刘福霞,刘乃森.不同光质对生菜幼苗生长的影响[J].北方园艺,2014,38(21):53.
　LIU Fu-xia,LIU Nai-sen.Effect of Different Light Qualities on Growth of Lettuce Seedlings[J].Northern Horticulture,2014,38(02):53.
[7]郭晓博,张晓丽,李俊华,等.山药愈伤组织研究进展[J].北方园艺,2014,38(24):183.
　GUO Xiao-bo,ZHANG Xiao-li,LI Jun-hua,et al.Research Advances on Callus Production in Dioscorea opposite[J].Northern Horticulture,2014,38(02):183.
[8]张 立 伟,刘 世 琦,张 自 坤,等.不同光质对豌豆苗品质的动态影响[J].北方园艺,2010,34(08):0.[doi:10.11937/bfyy.201008002]
　ZHANG Li -wei,LIU Shi-qi,ZHANG Zi -kun,et al.Dynamic Effects of Different Light Qualities on Pea Sprouts Quality[J].Northern Horticulture,2010,34(02):0.[doi:10.11937/bfyy.201008002]
[9]王婷婷,俞少娟,李鑫磊,等.LED光源对植物生理生化及品质影响研究进展[J].北方园艺,2016,40(02):189.[doi:10.11937/bfyy.201602050]
　WANG Tingting,YU Shaojuan,LI Xinlei,et al.Research Progress of Effect of LED Light on Plant Physiological Characteristics and Quality[J].Northern Horticulture,2016,40(02):189.[doi:10.11937/bfyy.201602050]
[10]骆萌,王杰,李玉玲,等.“新郁”葡萄离体再生体系的建立[J].北方园艺,2016,40(09):109.[doi:10.11937/bfyy.201609029]
　LUO Meng,WANG Jie,LI Yuling,et al.Establishment of Regeneration System of ‘Xinyu’ Grapevine in vitro[J].Northern Horticulture,2016,40(02):109.[doi:10.11937/bfyy.201609029]

备注/Memo

第一作者简介：王多佳（1982-），女，博士，讲师，研究方向为遗传学与分子生物学。E-mail:wduojia2009@163.com.责任作者：胡宝忠(1962-),男,教授,研究方向为植物学。E-mail：bzhu@neau.edu.cn.基金项目：高〖JP〗等学校博士后专项科研基金资助项目（200802240008）；黑龙江省自然科学基金资助项目（230340）。

更新日期/Last Update: 2015-02-24