HE Wen-bing,XIA Guang-hui,LIU Huan,et al.Optimization Extraction Conditions of Apigenin of Oenanthe javanica (Bl.) DC. and Antioxidant Activity[J].Northern Horticulture,2015,39(01):122-127.[doi:10.11937/bfyy.201501034]
野生水芹总黄酮提取工艺优化及抗氧化活性
- Title:
- Optimization Extraction Conditions of Apigenin of Oenanthe javanica (Bl.) DC. and Antioxidant Activity
- Keywords:
- Keywords: Oenanthe javanica (Bl.) DC.; total flavonoids; extraction process; optimization; antioxidant activity
- 文献标志码:
- A
- 摘要:
- 摘 要：以长白山区野生水芹为试材，在单因素试验的基础上，通过响应面法优化超声波提取野生水芹总黄酮工艺，并对其抗氧化活性进行考察。结果表明：最佳工艺条件为提取温度 70℃、提取功率60 W、提取时间9.19 min，在此条件下，水芹总黄酮提取率为2.9668 mg/g，验证试验得出水芹总黄酮提取率为2.9650 mg/g。水芹总黄酮对羟自由基具有较强的清除能力,能很好抑制油脂氧化。利用响应面分析法可优化水芹总黄酮提取工艺，得到理论最佳提取条件，验证结果表明该方法科学、高效。
- Abstract:
- Abstract:Taking Oenanthe javanica (Bl.) growing in Changbai mountain as material，based on single factor experiments，response surface methodology was used to optimize the ultrasonic-assisted extraction flavonoids，and antioxidant activity of total flavonoids were studied.The results showed that,the optimal extraction conditions were extraction temperature 70℃；extraction power 60 W；extraction time 9.19 minutes.Under these conditions，the predicted and experimental extraction yield of apigenin was 2.9668 mg/g and 2.9650 mg/g.Extracted total flavonoids had strong scavenging capacity on hydroxyl free base radicals and could better control oil oxidant.In conclusion，response surface method could provide a reliable approach to find optimal conditions for the extraction apigenin from Oenanthe javanica .The results suggested that the new method was effective and economical.
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备注/Memo
第一作者简介：何文兵(1977-)，男，博士研究生，副教授，现主要从事食品科学及果蔬功能性成分及风味物质等研究工作。E-mail：hwber@126.com.基金项目：吉林省科技发展计划资助项目(2013419008CB)；吉林省科技发展计划资助项目(20110268)；吉林省教育厅“十二五”科学技术研究资助项目(吉教科合字[2013]第495号)；通化师范学院校级资助项目（201270）。