DENG Jia,WANG Fang,SHI Zheng-jun,et al.Study on Bacteriostatic Activity of Different Parts of Calotropis gigantean Liposoluble Extraction[J].Northern Horticulture,2014,38(24):144-146.
野生牛角瓜不同部位脂溶性提取物抑菌活性的研究
- Title:
- Study on Bacteriostatic Activity of Different Parts of Calotropis gigantean Liposoluble Extraction
- 文章编号:
- 1001-0009（2014）24-0144-03
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以野生牛角瓜为试材，采用索氏提取法提取野生牛角瓜花、果、叶中脂溶性物质，选择大肠杆菌、普通变形杆菌和枯草芽孢杆菌为供试菌，采用抑菌圈法和最低抑菌浓度法测定其抑菌活性，研究野生牛角瓜不同部位脂溶性提取物的抑菌活性。结果表明：野生牛角瓜不同部位脂溶性提取物对大肠杆菌抑菌效果最明显，其中叶脂溶性提取物的抑菌活性最强。叶脂溶性提取物对大肠杆菌和普通变形杆菌的最小抑菌浓度分别为30、60 mg/mL。野生牛角瓜脂溶性提取物具有抗菌能力，可为牛角瓜资源的进一步开发利用提供一定的参考依据。
- Abstract:
- Taking Calotropis gigantean as material，liposoluble constituents of Calotropis gigantean’s flower，fruit and leave were extracted by soxhlet extraction.The antibacterial activities on Escherichia coli，Proteus vulgaris and Bacillus subtilis of three fractions were studied by inhibition zone method and minimal inhibitory concentration (MIC) method to study the bacteriostatic activity of different parts of Calotropis gigantean liposoluble extraction.The results showed that the antibacterial activity of liposoluble extraction in different parts of Calotropis gigantean were the highest for Escherichia coli，while leave extraction exhibited the strongest bacteriostatic activity.The minimal inhibitory concentration (MIC) of leaves liposoluble extraction against for Escherichia coli and Proteus vulgaris were 30 mg/mL，60 mg/mL respectively.The wild Calotropis gigantean liposoluble extractions had a good bacteriostatic activity.The study could provide theoretical foundation for further studying and developing of the Calotropis gigantean.
参考文献/References:
[1]中国科学院华南植物研究所编辑委员会.海南植物志[M].北京:科学出版社,1974:260-261. [2]戴好富,王茂媛,梅文莉,等.牛角瓜属植物化学成分与药理活性研究进展[J].河南大学学报(医学版),2009,28(1):1-7. [3]Singh S,Singh S,Singh A P.Phytochemical investigation of different plant parts of Calotropic Gigantea[J].International Journal of Scientific and Research Publication,2013,9(3):1-3. [4]Wang M Y,Mei W L,Deng Y Y,et al.Cytotoxic constituents from the roots of?Calotropis gigantean[J].Modern Pharmaceutical Research,2008,1(2):4-9. [5]Chitme H R,Chandra R,Kaushik S.Studies on anti-diarrhoeal activity of Calotropis gigantea R.Br.in experimental animals[J].Journal of Pharmacy and Pharmaceutical Sciences,2004,7(1):70-75. [6]王茂媛,王建荣,张新蕊,等.牛角瓜花脂溶性成分及其抗菌活性研究[J].中药材,2013,36(3):404-407. [7]Alam A M,Habib R M,Nikkon F,et al.Antimicrobial activity of akanda (Calotropis gigantean L.) on some pathogenic bacteria[J].Bangladesh Journal of Scientific and Industrial Research,2008,43(3):397-404. [8]张虹,白红丽,张江梅,等.青叶胆不同部位总黄酮提取及其抑菌作用[J].江苏农业科学,2013,41(9):224-226. [9]冯聪,林莉莉,陆军,等.红薯茎叶黄酮提取物抑菌研究[J].食品研究与开发,2011,32(8):14-17. [10]王茂媛,戴好富,王祝年.牛角瓜根脂溶性成分的GC-MS分析[J].热带作物学报,2010,31(11):2039-2042. [11]Seeka C,Sutthivaiyakit S.Cytotoxic cardenolides from the leaves of Calotropis gigantea[J].Chemical and Pharmaceutical Bulletin,2010,58(5):725-728. [12]Dhivya D,Manimegalai K.Preliminary phytochemical screening and GC-MS profiling of ethanolic flower extract of Calotropis gigantean Linn.(Apocyanaceae)[J].Journal of Pharmacognosy and Phytochemistry,2013,2(3):28-32.
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.Ｓ ｔ ｕ ｄ ｙ ｏ ｎ Ｖ ａ ｌ ｕ ｅ ｆ ｏ ｒ Ｄ ｅ ｖ ｅ ｌ ｏ ｐ ｍ ｅ ｎ ｔ ａ ｎ ｄ Ｃ ｕ ｌ ｔ ｉ ｖ ａ ｔ ｉ ｏ ｎＴ ｅ ｃ ｈ ｎ ｉ ｑ ｕ ｅ ｓ ｉ ｎ Ｃ ａ ｌ ｏ ｔ ｒ ｏ ｐ ｉ ｓ ｇ ｉ ｇ ａ ｎ ｔ ｅ ａ ｎ[J].Northern Horticulture,2011,35(24):0.[doi:10.11937/bfyy.2011018070]
备注/Memo
第一作者简介：邓佳（1983-），女，云南昆明人，博士，讲师，现主要从事经济林栽培与开发利用等研究工作。E-mail：dengjia1983@163.com.