LI Miao,LI Guo-qi.Comparative Research of Total RNA Extraction Methods From Apocynum venetum L.[J].Northern Horticulture,2014,38(11):93-96.
罗布麻总RNA提取方法比较研究
- Title:
- Comparative Research of Total RNA Extraction Methods From Apocynum venetum L.
- 文章编号:
- 1001-0009(2014)11-0093-04
- 关键词:
- 罗布麻; Trizol法; 改进SDS法; RNA plant plus Reagent试剂盒法; 总RNA提取
- Keywords:
- Apocynum venetum L.; Trizol method; modified SDS method; RNA plant plus Reagent Kit method; extraction of total RNA
- 分类号:
- S 563.7
- 文献标志码:
- A
- 摘要:
- 以Trizol法、改进SDS法及RNA plant plus Reagent试剂盒法提取罗布麻的总RNA，并通过紫外分光光度法和凝胶电泳检测对其提取效果进行比较分析，以筛选出罗布麻总RNA最佳提取方法。结果表明：Trizol法无法有效获得罗布麻总RNA，有较为显著的降解现象；改进SDS法提取的罗布麻RNA总量较少、浓度低，且试验重复性差；RNA plant plus Reagent试剂盒法提取的RNA质量好，纯度高，完整性强，可直接作为后续相关分子生物学试验材料。
- Abstract:
- Total RNA was extracted by Trizol,modified SDS and RNA plant plus Reagent Kit methods,the extracted effect were compared by UV Spectrophotometry and Gel Electrophoresis,in order to screen out the best method for extracting Apocynum venetum L..The results showed that the RNA samples extracted by the method of Trizol were degraded gravely.Samples extracted by the method of modified SDS were low concentration and less amount,and the operation results were in poor repeatability.RNA plant plus Reagent Kit method was optimum extraction of A.venetum L. total RNA.The RNA samples extracted by the method of RNA plant plus Reagent Kit method were high quality,purity and good reproducibility,and could be used for research of molecule biology directly.
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备注/Memo
第一作者简介：李苗（1982-），男，宁夏银川人，硕士研究生，助理研究员，现主要从事植物生态及特色植物资源利用等研究工作。E-mail：limiao1228@sina.com.
责任作者：李国旗（1965-），男，宁夏银川人，博士，研究员，研究方向为植物生态学。
基金项目：国家科技支撑计划资助项目（2011BAC07B03）。
收稿日期：2014-01-20