ZHANG Li-xia,GUO Li-ping.Influence Factors of Rapid Propagation of Huazi Diosorea opposita Thunb. Plantlets in vitro [J].Northern Horticulture,2014,38(08):80-82.
影响花籽山药脱毒试管苗快繁的因素
- Title:
- Influence Factors of Rapid Propagation of Huazi Diosorea opposita Thunb. Plantlets in vitro
- 文章编号:
- 1001-0009（2014）08-0080-03
- Keywords:
- Huazi Diosorea opposita Thunb.; test-tube plantlet; rapid propagation; the plant growth substances; light; training way
- 分类号:
- S 632.1
- 文献标志码:
- A
- 摘要:
- 以花籽山药为试材，研究了植物生长物质、光（或光质）、培养方式等因素对花籽山药试管苗快繁的影响。结果表明:最适宜的培养基为MS＋激动素（KT） 2.0 mg/L＋萘乙酸（NAA） 0.02 mg/L＋多效唑（PP333） ?0.1 mg/L；最适宜的光质和培养方式为在蓝光和白光下的液体培养，且液体摇床振荡培养后静止培养的效果较好；此外，带叶茎段较不带叶茎段繁殖系数高。
- Abstract:
- Taking Huazi Diosorea opposita Thunb. as test material，the effect of plant growth substances，light (or light quality)，develop methods for Huazi Diosorea opposita Thunb. plantlets propagation were studied.The results showed that:the most suitable medium was MS+KT 2.0 mg/L+NAA 0.02 mg/L+PP333 0.1 mg/L;the most appropriate way for the light quality and training of liquid culture were under blue and white，the effect of the static culture after the liquid shaking culture was better;in addition，stem with leaf had higher propagation coefficient than stem with no leaf.
参考文献/References:
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备注/Memo
第一作者简介：张丽霞（1972-），女，河南内黄人，硕士，副教授，现主要从事生物化学和遗传育种等教学与科研工作。E-mail:aygxyzlx@163.com.