YIN Dong-lin,DUAN Hong-bin,WANG Rui-li.Study on the Sexual Reproduction of Cordyceps militaris[J].Northern Horticulture,2014,38(03):130-133.
蛹虫草有性生殖研究
- Title:
- Study on the Sexual Reproduction of Cordyceps militaris
- 文章编号:
- 1001-0009(2014)03-0130-04
- Keywords:
- Cordyceps militaris; ascomycetes spores; confrontation culture; fan-shaped hybrid zone; bipolar heterothallism
- 分类号:
- Q 93
- 文献标志码:
- A
- 摘要:
- 从2种人工栽培的蛹虫草子实体上分离子囊单孢子，将单孢子单独培养和两两配对培养来诱导产生子实体，以研究蛹虫草有性生殖机理。结果表明：蛹虫草有性生殖方式存在多样化，除了具有典型的二极异宗配合外，还存在可孕单孢子。将单孢子对峙培养结果发现在两单孢子间有扇形杂交区的配对培养产生子实体的能力强，没有扇形杂交区的两单孢子配对培养产生子实体的能力弱。
- Abstract:
- Ascomycetous single spores were isolated from the entity of Cordyceps militarisby two kinds of artificial cultivation,the single spore isolated and two-two pairs culture to induce to fruiting body,in order to study the mechanism on sexual reproduction of Cordyceps militaris.The results showed that Cordyceps sexual reproduction could be diversified，in addition to the typical bipolar heterothallism，there was fertile spores.The single spore confrontation culture results found that the ability of two single spore with fan-shaped hybrid zone pairing culture to produce fruiting body was strong，the ability of no fan-shaped hybrid zone two single spore mating to produce fruiting body was weak.
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备注/Memo
第一作者简介：殷东林（1983-），男，硕士，讲师，现主要从事生物技术的教学与科研工作。