MO Xi-li,ZHAO Tong-gui,DENG Wei,et al.Effect of Extract of Zanthoxylum bungeanum on Pathogen Inhibition and Activities of Antioxidative Enzyme of Monilinia fructicola in Peach[J].Northern Horticulture,2014,38(20):129-132.
花椒提取物对桃褐腐病菌的抑制作用及酶活性的影响
- Title:
- Effect of Extract of Zanthoxylum bungeanum on Pathogen Inhibition and Activities of Antioxidative Enzyme of Monilinia fructicola in Peach
- 文章编号:
- 1001-0009（2014）20-0129-04
- 关键词:
- 花椒; 桃褐腐病; 苯丙氨酸解氨酶（PAL）; 过氧化物酶(POD); 多酚氧化酶(PPO)
- Keywords:
- Zanthoxylum bungeanum; Monilinia fructicola; phenylalanine ammoniumlyase(PAL); peroxidase(POD); polyphenol oxidase(PPO)
- 分类号:
- S 436.6
- 文献标志码:
- A
- 摘要:
- 以花椒提取物为试材，采用生长速率法测定了不同浓度的花椒提取物对桃褐腐病菌的抑制效果；并对处理后桃果实的苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）、多酚氧化酶（PPO）活性进行检测。结果表明：不同浓度的花椒提取物对褐腐病菌菌丝的生长表现出较强的抑制效果，浓度为5.00 mg/mL时抑制效果最好，抑制生长率达到96.71%，其EC50为0.504 mg/mL；桃果实经提取物处理后，能显著诱导果实中抗病相关酶PAL、POD、PPO活性升高，并在整个试验过程中保持较高的水平。花椒提取物对桃褐腐病菌具有一定的抑制作用和诱导桃果实产生抗性的作用。
- Abstract:
- Taking extract of Zanthoxylum bungeanum as material，the mycelium growth rate method was used to test the inhibitory effect of extracts with different concentrations on Monilinia fructicola；the activities of phenylalanine ammoniumlyase(PAL)，peroxidease(POD）and polyphenol oxidase(PPO）induced by inoculation with extract were studied in peach.The results showed that the extract of Zanthoxylum bungeanum displayed strong antifungal activity to M.fructicola.When the concentration of extracts was 5.00 mg/mL，the inhibitory effect was best；the inhibitory rate was 96.71%.The EC50 value was 0.504 mg/mL.The activities of PAL，POD and PPO in peach increased significantly when inoculated with extract and M.fructicola，and maintained at a high level throughout the experiment.The extract of Zanthoxylum bungeanum had antifungal activity and exerted a potent impact on inducing resistance of peach to M.fructicola.
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备注/Memo
第一作者简介：莫熙礼（1982-），男，广西梧州人，硕士，讲师，现主要从事植物病虫害防治等教学与科研工作。E-mail:moxili1982@163.com.
基金项目：黔西南州科技局计划资助项目（2010-14）。
收稿日期：2014-05-22